Application of spatial transcriptome technologies to neurological diseases
Spatial transcriptome technology acquires gene expression profiles while retaining spatial location information, it displays the gene expression properties of cells in situ. Through the investigation of cell heterogeneity, microenvironment, function, and cellular interactions, spatial transcriptome...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Cell and Developmental Biology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2023.1142923/full |
| _version_ | 1827999811252518912 |
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| author | Dongshan Ya Dongshan Ya Yingmei Zhang Yingmei Zhang Qi Cui Qi Cui Yanlin Jiang Jiaxin Yang Jiaxin Yang Ning Tian Ning Tian Wenjing Xiang Xiaohui Lin Qinghua Li Qinghua Li Qinghua Li Rujia Liao Rujia Liao Rujia Liao |
| author_facet | Dongshan Ya Dongshan Ya Yingmei Zhang Yingmei Zhang Qi Cui Qi Cui Yanlin Jiang Jiaxin Yang Jiaxin Yang Ning Tian Ning Tian Wenjing Xiang Xiaohui Lin Qinghua Li Qinghua Li Qinghua Li Rujia Liao Rujia Liao Rujia Liao |
| author_sort | Dongshan Ya |
| collection | DOAJ |
| description | Spatial transcriptome technology acquires gene expression profiles while retaining spatial location information, it displays the gene expression properties of cells in situ. Through the investigation of cell heterogeneity, microenvironment, function, and cellular interactions, spatial transcriptome technology can deeply explore the pathogenic mechanisms of cell-type-specific responses and spatial localization in neurological diseases. The present article overviews spatial transcriptome technologies based on microdissection, in situ hybridization, in situ sequencing, in situ capture, and live cell labeling. Each technology is described along with its methods, detection throughput, spatial resolution, benefits, and drawbacks. Furthermore, their applications in neurodegenerative disease, neuropsychiatric illness, stroke and epilepsy are outlined. This information can be used to understand disease mechanisms, pick therapeutic targets, and establish biomarkers. |
| first_indexed | 2024-04-10T06:04:20Z |
| format | Article |
| id | doaj.art-d651718b627640c69f455f45bfed1d80 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-04-10T06:04:20Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-d651718b627640c69f455f45bfed1d802023-03-03T04:50:07ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2023-03-011110.3389/fcell.2023.11429231142923Application of spatial transcriptome technologies to neurological diseasesDongshan Ya0Dongshan Ya1Yingmei Zhang2Yingmei Zhang3Qi Cui4Qi Cui5Yanlin Jiang6Jiaxin Yang7Jiaxin Yang8Ning Tian9Ning Tian10Wenjing Xiang11Xiaohui Lin12Qinghua Li13Qinghua Li14Qinghua Li15Rujia Liao16Rujia Liao17Rujia Liao18Laboratory of Neuroscience, Affiliated Hospital of Guilin Medical University, Guilin Medical University, Guilin, ChinaDepartment of Neurology, Affiliated Hospital of Guilin Medical University, Guilin Medical University, Guilin, ChinaLaboratory of Neuroscience, Affiliated Hospital of Guilin Medical University, Guilin Medical University, Guilin, ChinaDepartment of Neurology, Affiliated Hospital of Guilin Medical University, Guilin Medical University, Guilin, ChinaLaboratory of Neuroscience, Affiliated Hospital of Guilin Medical University, Guilin Medical University, Guilin, ChinaDepartment of Neurology, Affiliated Hospital of Guilin Medical University, Guilin Medical University, Guilin, ChinaDepartment of Pharmacology, Affiliated Hospital of Guilin Medical University, Guilin Medical University, Guilin, ChinaLaboratory of Neuroscience, Affiliated Hospital of Guilin Medical University, Guilin Medical University, Guilin, ChinaDepartment of Neurology, Affiliated Hospital of Guilin Medical University, Guilin Medical University, Guilin, ChinaLaboratory of Neuroscience, Affiliated Hospital of Guilin Medical University, Guilin Medical University, Guilin, ChinaGuangxi Clinical Research Center for Neurological Diseases, Affiliated Hospital of Guilin Medical University, Guilin Medical University, Guilin, ChinaDepartment of Neurology ward 2, Guilin People’s Hospital, Guilin, ChinaDepartment of Geriatrics, Affiliated Hospital of Guilin Medical University, Guilin Medical University, Guilin, ChinaLaboratory of Neuroscience, Affiliated Hospital of Guilin Medical University, Guilin Medical University, Guilin, ChinaDepartment of Neurology, Affiliated Hospital of Guilin Medical University, Guilin Medical University, Guilin, ChinaGuangxi Clinical Research Center for Neurological Diseases, Affiliated Hospital of Guilin Medical University, Guilin Medical University, Guilin, ChinaLaboratory of Neuroscience, Affiliated Hospital of Guilin Medical University, Guilin Medical University, Guilin, ChinaDepartment of Neurology, Affiliated Hospital of Guilin Medical University, Guilin Medical University, Guilin, ChinaGuangxi Clinical Research Center for Neurological Diseases, Affiliated Hospital of Guilin Medical University, Guilin Medical University, Guilin, ChinaSpatial transcriptome technology acquires gene expression profiles while retaining spatial location information, it displays the gene expression properties of cells in situ. Through the investigation of cell heterogeneity, microenvironment, function, and cellular interactions, spatial transcriptome technology can deeply explore the pathogenic mechanisms of cell-type-specific responses and spatial localization in neurological diseases. The present article overviews spatial transcriptome technologies based on microdissection, in situ hybridization, in situ sequencing, in situ capture, and live cell labeling. Each technology is described along with its methods, detection throughput, spatial resolution, benefits, and drawbacks. Furthermore, their applications in neurodegenerative disease, neuropsychiatric illness, stroke and epilepsy are outlined. This information can be used to understand disease mechanisms, pick therapeutic targets, and establish biomarkers.https://www.frontiersin.org/articles/10.3389/fcell.2023.1142923/fullspatial transcriptomeneurodegenerative diseaseneuropsychiatric illnessstrokeepilepsy |
| spellingShingle | Dongshan Ya Dongshan Ya Yingmei Zhang Yingmei Zhang Qi Cui Qi Cui Yanlin Jiang Jiaxin Yang Jiaxin Yang Ning Tian Ning Tian Wenjing Xiang Xiaohui Lin Qinghua Li Qinghua Li Qinghua Li Rujia Liao Rujia Liao Rujia Liao Application of spatial transcriptome technologies to neurological diseases Frontiers in Cell and Developmental Biology spatial transcriptome neurodegenerative disease neuropsychiatric illness stroke epilepsy |
| title | Application of spatial transcriptome technologies to neurological diseases |
| title_full | Application of spatial transcriptome technologies to neurological diseases |
| title_fullStr | Application of spatial transcriptome technologies to neurological diseases |
| title_full_unstemmed | Application of spatial transcriptome technologies to neurological diseases |
| title_short | Application of spatial transcriptome technologies to neurological diseases |
| title_sort | application of spatial transcriptome technologies to neurological diseases |
| topic | spatial transcriptome neurodegenerative disease neuropsychiatric illness stroke epilepsy |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2023.1142923/full |
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