Comparison of miRNA transcriptome of exosomes in three categories of somatic cells with derived iPSCs
Abstract Somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) through epigenetic manipulation. While the essential role of miRNA in reprogramming and maintaining pluripotency is well studied, little is known about the functions of miRNA from exosomes in this context. To fill...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-09-01
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| Series: | Scientific Data |
| Online Access: | https://doi.org/10.1038/s41597-023-02493-5 |
| _version_ | 1797453973274230784 |
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| author | Chunlai Yu Mei Zhang Yucui Xiong Qizheng Wang Yuanhua Wang Shaoling Wu Sajjad Hussain Yan Wang Zhizhong Zhang Nini Rao Sheng Zhang Xiao Zhang |
| author_facet | Chunlai Yu Mei Zhang Yucui Xiong Qizheng Wang Yuanhua Wang Shaoling Wu Sajjad Hussain Yan Wang Zhizhong Zhang Nini Rao Sheng Zhang Xiao Zhang |
| author_sort | Chunlai Yu |
| collection | DOAJ |
| description | Abstract Somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) through epigenetic manipulation. While the essential role of miRNA in reprogramming and maintaining pluripotency is well studied, little is known about the functions of miRNA from exosomes in this context. To fill this research gap,we comprehensively obtained the 17 sets of cellular mRNA transcriptomic data with 3.93 × 1010 bp raw reads and 18 sets of exosomal miRNA transcriptomic data with 2.83 × 107 bp raw reads from three categories of human somatic cells: peripheral blood mononuclear cells (PBMCs), skin fibroblasts(SFs) and urine cells (UCs), along with their derived iPSCs. Additionally, differentially expressed molecules of each category were identified and used to perform gene set enrichment analysis. Our study provides sets of comparative transcriptomic data of cellular mRNA and exosomal miRNA from three categories of human tissue with three individual biological controls in studies of iPSCs generation, which will contribute to a better understanding of donor cell variation in functional epigenetic regulation and differentiation bias in iPSCs. |
| first_indexed | 2024-03-09T15:30:37Z |
| format | Article |
| id | doaj.art-d652f440af4c4ea6aae136e4d4af1eb6 |
| institution | Directory Open Access Journal |
| issn | 2052-4463 |
| language | English |
| last_indexed | 2024-03-09T15:30:37Z |
| publishDate | 2023-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Data |
| spelling | doaj.art-d652f440af4c4ea6aae136e4d4af1eb62023-11-26T12:18:45ZengNature PortfolioScientific Data2052-44632023-09-0110111210.1038/s41597-023-02493-5Comparison of miRNA transcriptome of exosomes in three categories of somatic cells with derived iPSCsChunlai Yu0Mei Zhang1Yucui Xiong2Qizheng Wang3Yuanhua Wang4Shaoling Wu5Sajjad Hussain6Yan Wang7Zhizhong Zhang8Nini Rao9Sheng Zhang10Xiao Zhang11University of Electronic Science and Technology of ChinaBinzhou Medical UniversityGuangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesGuangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesGuangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesDepartment of Rehabilitation Medicine, Sun Yat-sen Memorial Hospital, Sun Yat-sen UniversityGuangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesGuangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesGuangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesUniversity of Electronic Science and Technology of ChinaGuangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesGuangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesAbstract Somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) through epigenetic manipulation. While the essential role of miRNA in reprogramming and maintaining pluripotency is well studied, little is known about the functions of miRNA from exosomes in this context. To fill this research gap,we comprehensively obtained the 17 sets of cellular mRNA transcriptomic data with 3.93 × 1010 bp raw reads and 18 sets of exosomal miRNA transcriptomic data with 2.83 × 107 bp raw reads from three categories of human somatic cells: peripheral blood mononuclear cells (PBMCs), skin fibroblasts(SFs) and urine cells (UCs), along with their derived iPSCs. Additionally, differentially expressed molecules of each category were identified and used to perform gene set enrichment analysis. Our study provides sets of comparative transcriptomic data of cellular mRNA and exosomal miRNA from three categories of human tissue with three individual biological controls in studies of iPSCs generation, which will contribute to a better understanding of donor cell variation in functional epigenetic regulation and differentiation bias in iPSCs.https://doi.org/10.1038/s41597-023-02493-5 |
| spellingShingle | Chunlai Yu Mei Zhang Yucui Xiong Qizheng Wang Yuanhua Wang Shaoling Wu Sajjad Hussain Yan Wang Zhizhong Zhang Nini Rao Sheng Zhang Xiao Zhang Comparison of miRNA transcriptome of exosomes in three categories of somatic cells with derived iPSCs Scientific Data |
| title | Comparison of miRNA transcriptome of exosomes in three categories of somatic cells with derived iPSCs |
| title_full | Comparison of miRNA transcriptome of exosomes in three categories of somatic cells with derived iPSCs |
| title_fullStr | Comparison of miRNA transcriptome of exosomes in three categories of somatic cells with derived iPSCs |
| title_full_unstemmed | Comparison of miRNA transcriptome of exosomes in three categories of somatic cells with derived iPSCs |
| title_short | Comparison of miRNA transcriptome of exosomes in three categories of somatic cells with derived iPSCs |
| title_sort | comparison of mirna transcriptome of exosomes in three categories of somatic cells with derived ipscs |
| url | https://doi.org/10.1038/s41597-023-02493-5 |
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