Gut Microbiome Was Highly Related to the Regulation of Metabolism in Lung Adenocarcinoma Patients
BackgroundLung adenocarcinoma (LUAD) is one of the most predominant subtypes of lung cancer. The gut microbiome plays a vital role in the pathophysiological processes of various diseases, including cancers.MethodsIn the study, 100 individuals were enrolled. In total 75 stool and blood samples were a...
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| Format: | Article |
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2022.790467/full |
| _version_ | 1818230772793868288 |
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| author | Sheng Wang Huachun Chen Huizhen Yang Kejin Zhou Fan Bai Xiaoyu Wu Hanwen Xu |
| author_facet | Sheng Wang Huachun Chen Huizhen Yang Kejin Zhou Fan Bai Xiaoyu Wu Hanwen Xu |
| author_sort | Sheng Wang |
| collection | DOAJ |
| description | BackgroundLung adenocarcinoma (LUAD) is one of the most predominant subtypes of lung cancer. The gut microbiome plays a vital role in the pathophysiological processes of various diseases, including cancers.MethodsIn the study, 100 individuals were enrolled. In total 75 stool and blood samples were analyzed with 16s-rRNA gene sequencing and metabolomics (30 from healthy individuals (H); 45 from LUAD patients). In addition, 25 stool samples were analyzed with metagenomics (10 from H; 15 from LUAD). The linear discriminant analysis (LDA) effect size (LefSe) and logistic regression analysis were applied to identify biomarkers’ taxa and develop a diagnostic model. The diagnostic power of the model was estimated with the receiver operating characteristic curve (ROC) by comparing the area under the ROC (AUC). The correlation between biomarker’s taxa and metabolites was calculated using the Spearman analysis.ResultsThe α and β diversity demonstrated the composition and structure of the gut microbiome in LUAD patients were different from those in healthy people. The top three abundance of genera were Bacteroides (25.06%), Faecalibacterium (11.00%), and Prevotella (5.94%). The LefSe and logistic regression analysis identified three biomarker taxa (Bacteroides, Pseudomonas, and Ruminococcus gnavus group) and constructed a diagnostic model. The AUCs of the diagnostic model in 16s-rRNA gene sequencing and metagenomics were 0.852 and 0.841, respectively. A total of 102 plasma metabolites were highly related to those three biomarkers’ taxa. Seven metabolic pathways were enriched by 102 plasma metabolites, including the Pentose phosphate pathway, Glutathione metabolism.ConclusionsIn LUAD patients, the gut microbiome profile has significantly changed. We used three biomarkers taxa to develop a diagnostic model, which was accurate and suitable for the diagnosis of LUAD. Gut microbes, especially those three biomarkers’ taxa, may participate in regulating metabolism-related pathways in LUAD patients, such as the pentose phosphate pathway and glutathione metabolism. |
| first_indexed | 2024-12-12T10:39:49Z |
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| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-12-12T10:39:49Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-d654f6f1dbc545389f5234cc4f3ed9ff2022-12-22T00:27:05ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2022-05-011210.3389/fonc.2022.790467790467Gut Microbiome Was Highly Related to the Regulation of Metabolism in Lung Adenocarcinoma PatientsSheng WangHuachun ChenHuizhen YangKejin ZhouFan BaiXiaoyu WuHanwen XuBackgroundLung adenocarcinoma (LUAD) is one of the most predominant subtypes of lung cancer. The gut microbiome plays a vital role in the pathophysiological processes of various diseases, including cancers.MethodsIn the study, 100 individuals were enrolled. In total 75 stool and blood samples were analyzed with 16s-rRNA gene sequencing and metabolomics (30 from healthy individuals (H); 45 from LUAD patients). In addition, 25 stool samples were analyzed with metagenomics (10 from H; 15 from LUAD). The linear discriminant analysis (LDA) effect size (LefSe) and logistic regression analysis were applied to identify biomarkers’ taxa and develop a diagnostic model. The diagnostic power of the model was estimated with the receiver operating characteristic curve (ROC) by comparing the area under the ROC (AUC). The correlation between biomarker’s taxa and metabolites was calculated using the Spearman analysis.ResultsThe α and β diversity demonstrated the composition and structure of the gut microbiome in LUAD patients were different from those in healthy people. The top three abundance of genera were Bacteroides (25.06%), Faecalibacterium (11.00%), and Prevotella (5.94%). The LefSe and logistic regression analysis identified three biomarker taxa (Bacteroides, Pseudomonas, and Ruminococcus gnavus group) and constructed a diagnostic model. The AUCs of the diagnostic model in 16s-rRNA gene sequencing and metagenomics were 0.852 and 0.841, respectively. A total of 102 plasma metabolites were highly related to those three biomarkers’ taxa. Seven metabolic pathways were enriched by 102 plasma metabolites, including the Pentose phosphate pathway, Glutathione metabolism.ConclusionsIn LUAD patients, the gut microbiome profile has significantly changed. We used three biomarkers taxa to develop a diagnostic model, which was accurate and suitable for the diagnosis of LUAD. Gut microbes, especially those three biomarkers’ taxa, may participate in regulating metabolism-related pathways in LUAD patients, such as the pentose phosphate pathway and glutathione metabolism.https://www.frontiersin.org/articles/10.3389/fonc.2022.790467/fullgut microbiomemetabolismlung adenocarcinomagut-lung axisdiagnosis |
| spellingShingle | Sheng Wang Huachun Chen Huizhen Yang Kejin Zhou Fan Bai Xiaoyu Wu Hanwen Xu Gut Microbiome Was Highly Related to the Regulation of Metabolism in Lung Adenocarcinoma Patients Frontiers in Oncology gut microbiome metabolism lung adenocarcinoma gut-lung axis diagnosis |
| title | Gut Microbiome Was Highly Related to the Regulation of Metabolism in Lung Adenocarcinoma Patients |
| title_full | Gut Microbiome Was Highly Related to the Regulation of Metabolism in Lung Adenocarcinoma Patients |
| title_fullStr | Gut Microbiome Was Highly Related to the Regulation of Metabolism in Lung Adenocarcinoma Patients |
| title_full_unstemmed | Gut Microbiome Was Highly Related to the Regulation of Metabolism in Lung Adenocarcinoma Patients |
| title_short | Gut Microbiome Was Highly Related to the Regulation of Metabolism in Lung Adenocarcinoma Patients |
| title_sort | gut microbiome was highly related to the regulation of metabolism in lung adenocarcinoma patients |
| topic | gut microbiome metabolism lung adenocarcinoma gut-lung axis diagnosis |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2022.790467/full |
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