Prevention of poxvirus infection by tetrapyrroles
<p>Abstract</p> <p>Background</p> <p>Prevention of poxvirus infection is a topic of great current interest. We report inhibition of vaccinia virus in cell culture by porphyrins and phthalocyanines. Most previous work on the inhibition of viruses with tetrapyrroles has i...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2003-05-01
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| Series: | BMC Infectious Diseases |
| Online Access: | http://www.biomedcentral.com/1471-2334/3/9 |
| _version_ | 1811286641078173696 |
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| author | Marzilli Luigi G Vzorov Andrei N Dixon Dabney W Chen-Collins Avril RM Compans Richard W |
| author_facet | Marzilli Luigi G Vzorov Andrei N Dixon Dabney W Chen-Collins Avril RM Compans Richard W |
| author_sort | Marzilli Luigi G |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Prevention of poxvirus infection is a topic of great current interest. We report inhibition of vaccinia virus in cell culture by porphyrins and phthalocyanines. Most previous work on the inhibition of viruses with tetrapyrroles has involved photodynamic mechanisms. The current study, however, investigates light-independent inhibition activity.</p> <p>Methods</p> <p>The Western Reserve (WR) and International Health Department-J (IHD-J) strains of vaccinia virus were used. Virucidal and antiviral activities as well as the cytotoxicity of test compounds were determined.</p> <p>Results</p> <p>Examples of active compounds include zinc protoporphyrin, copper hematoporphyrin, <it>meso</it>(2,6-dihydroxyphenyl)porphyrin, the sulfonated tetra-1-naphthyl and tetra-1-anthracenylporphyrins, selected sulfonated derivatives of halogenated tetraphenyl porphyrins and the copper chelate of tetrasulfonated phthalocyanine. EC<sub>50 </sub>values for the most active compounds are as low as 0.05 µg/mL (40 nM). One of the most active compounds was the neutral <it>meso</it>(2,6-dihydroxyphenyl)porphyrin, indicating that the compounds do not have to be negatively charged to be active.</p> <p>Conclusions</p> <p>Porphyrins and phthalocyanines have been found to be potent inhibitors of infection by vaccinia virus in cell culture. These tetrapyrroles were found to be active against two different virus strains, and against both enveloped and non-enveloped forms of the virus, indicating that these compounds may be broadly effective in their ability to inhibit poxvirus infection.</p> |
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| format | Article |
| id | doaj.art-d65640bc63ef4b06a39291931f781d50 |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-04-13T03:04:38Z |
| publishDate | 2003-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-d65640bc63ef4b06a39291931f781d502022-12-22T03:05:19ZengBMCBMC Infectious Diseases1471-23342003-05-01319Prevention of poxvirus infection by tetrapyrrolesMarzilli Luigi GVzorov Andrei NDixon Dabney WChen-Collins Avril RMCompans Richard W<p>Abstract</p> <p>Background</p> <p>Prevention of poxvirus infection is a topic of great current interest. We report inhibition of vaccinia virus in cell culture by porphyrins and phthalocyanines. Most previous work on the inhibition of viruses with tetrapyrroles has involved photodynamic mechanisms. The current study, however, investigates light-independent inhibition activity.</p> <p>Methods</p> <p>The Western Reserve (WR) and International Health Department-J (IHD-J) strains of vaccinia virus were used. Virucidal and antiviral activities as well as the cytotoxicity of test compounds were determined.</p> <p>Results</p> <p>Examples of active compounds include zinc protoporphyrin, copper hematoporphyrin, <it>meso</it>(2,6-dihydroxyphenyl)porphyrin, the sulfonated tetra-1-naphthyl and tetra-1-anthracenylporphyrins, selected sulfonated derivatives of halogenated tetraphenyl porphyrins and the copper chelate of tetrasulfonated phthalocyanine. EC<sub>50 </sub>values for the most active compounds are as low as 0.05 µg/mL (40 nM). One of the most active compounds was the neutral <it>meso</it>(2,6-dihydroxyphenyl)porphyrin, indicating that the compounds do not have to be negatively charged to be active.</p> <p>Conclusions</p> <p>Porphyrins and phthalocyanines have been found to be potent inhibitors of infection by vaccinia virus in cell culture. These tetrapyrroles were found to be active against two different virus strains, and against both enveloped and non-enveloped forms of the virus, indicating that these compounds may be broadly effective in their ability to inhibit poxvirus infection.</p>http://www.biomedcentral.com/1471-2334/3/9 |
| spellingShingle | Marzilli Luigi G Vzorov Andrei N Dixon Dabney W Chen-Collins Avril RM Compans Richard W Prevention of poxvirus infection by tetrapyrroles BMC Infectious Diseases |
| title | Prevention of poxvirus infection by tetrapyrroles |
| title_full | Prevention of poxvirus infection by tetrapyrroles |
| title_fullStr | Prevention of poxvirus infection by tetrapyrroles |
| title_full_unstemmed | Prevention of poxvirus infection by tetrapyrroles |
| title_short | Prevention of poxvirus infection by tetrapyrroles |
| title_sort | prevention of poxvirus infection by tetrapyrroles |
| url | http://www.biomedcentral.com/1471-2334/3/9 |
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