The Loading of Epigallocatechin Gallate on Bovine Serum Albumin and Pullulan-Based Nanoparticles as Effective Antioxidant
Due to its poor stability and rapid metabolism, the biological activity and absorption of epigallocatechin gallate (EGCG) is limited. In this work, EGCG-loaded bovine serum albumin (BSA)/pullulan (PUL) nanoparticles (BPENs) were successfully fabricated via self-assembly. This assembly was driven by...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/24/4074 |
| _version_ | 1827639521881096192 |
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| author | Zikun Li Xiaohan Wang Man Zhang Hongjun He Bin Liang Chanchan Sun Xiulian Li Changjian Ji |
| author_facet | Zikun Li Xiaohan Wang Man Zhang Hongjun He Bin Liang Chanchan Sun Xiulian Li Changjian Ji |
| author_sort | Zikun Li |
| collection | DOAJ |
| description | Due to its poor stability and rapid metabolism, the biological activity and absorption of epigallocatechin gallate (EGCG) is limited. In this work, EGCG-loaded bovine serum albumin (BSA)/pullulan (PUL) nanoparticles (BPENs) were successfully fabricated via self-assembly. This assembly was driven by hydrogen bonding, which provided the desired EGCG loading efficiency, high stability, and a strong antioxidant capacity. The encapsulation efficiency of the BPENs was above 99.0%. BPENs have high antioxidant activity in vitro, and, in this study, their antioxidant capacity increased with an increase in the EGCG concentration. The in vitro release assays showed that the BPENs were released continuously over 6 h. The Fourier transform infrared spectra (FTIR) analysis indicated the presence of hydrogen bonding, hydrophobic interactions, and electrostatic interactions, which were the driving forces for the formation of the EGCG carrier nanoparticles. Furthermore, the transmission electron microscope (TEM) images demonstrated that the BSA/PUL-based nanoparticles (BPNs) and BPENs both exhibited regular spherical particles. In conclusion, BPENs are good delivery carriers for enhancing the stability and antioxidant activity of EGCG. |
| first_indexed | 2024-03-09T16:41:30Z |
| format | Article |
| id | doaj.art-d65640c657ba4836b70718e7b125accc |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T16:41:30Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-d65640c657ba4836b70718e7b125accc2023-11-24T14:52:00ZengMDPI AGFoods2304-81582022-12-011124407410.3390/foods11244074The Loading of Epigallocatechin Gallate on Bovine Serum Albumin and Pullulan-Based Nanoparticles as Effective AntioxidantZikun Li0Xiaohan Wang1Man Zhang2Hongjun He3Bin Liang4Chanchan Sun5Xiulian Li6Changjian Ji7College of Life Sciences, Yantai University, Yantai 264005, ChinaCollege of Life Sciences, Yantai University, Yantai 264005, ChinaCollege of Life Sciences, Yantai University, Yantai 264005, ChinaCollege of Life Sciences, Yantai University, Yantai 264005, ChinaCollege of Food Engineering, Ludong University, Yantai 264025, ChinaCollege of Life Sciences, Yantai University, Yantai 264005, ChinaSchool of Pharmacy, Binzhou Medical University, Yantai 264003, ChinaDepartment of Physics and Electronic Engineering, Qilu Normal University, Jinan 250200, ChinaDue to its poor stability and rapid metabolism, the biological activity and absorption of epigallocatechin gallate (EGCG) is limited. In this work, EGCG-loaded bovine serum albumin (BSA)/pullulan (PUL) nanoparticles (BPENs) were successfully fabricated via self-assembly. This assembly was driven by hydrogen bonding, which provided the desired EGCG loading efficiency, high stability, and a strong antioxidant capacity. The encapsulation efficiency of the BPENs was above 99.0%. BPENs have high antioxidant activity in vitro, and, in this study, their antioxidant capacity increased with an increase in the EGCG concentration. The in vitro release assays showed that the BPENs were released continuously over 6 h. The Fourier transform infrared spectra (FTIR) analysis indicated the presence of hydrogen bonding, hydrophobic interactions, and electrostatic interactions, which were the driving forces for the formation of the EGCG carrier nanoparticles. Furthermore, the transmission electron microscope (TEM) images demonstrated that the BSA/PUL-based nanoparticles (BPNs) and BPENs both exhibited regular spherical particles. In conclusion, BPENs are good delivery carriers for enhancing the stability and antioxidant activity of EGCG.https://www.mdpi.com/2304-8158/11/24/4074bovine serum albuminpullulanepigallocatechin gallatenanoparticlesantioxidant activity |
| spellingShingle | Zikun Li Xiaohan Wang Man Zhang Hongjun He Bin Liang Chanchan Sun Xiulian Li Changjian Ji The Loading of Epigallocatechin Gallate on Bovine Serum Albumin and Pullulan-Based Nanoparticles as Effective Antioxidant Foods bovine serum albumin pullulan epigallocatechin gallate nanoparticles antioxidant activity |
| title | The Loading of Epigallocatechin Gallate on Bovine Serum Albumin and Pullulan-Based Nanoparticles as Effective Antioxidant |
| title_full | The Loading of Epigallocatechin Gallate on Bovine Serum Albumin and Pullulan-Based Nanoparticles as Effective Antioxidant |
| title_fullStr | The Loading of Epigallocatechin Gallate on Bovine Serum Albumin and Pullulan-Based Nanoparticles as Effective Antioxidant |
| title_full_unstemmed | The Loading of Epigallocatechin Gallate on Bovine Serum Albumin and Pullulan-Based Nanoparticles as Effective Antioxidant |
| title_short | The Loading of Epigallocatechin Gallate on Bovine Serum Albumin and Pullulan-Based Nanoparticles as Effective Antioxidant |
| title_sort | loading of epigallocatechin gallate on bovine serum albumin and pullulan based nanoparticles as effective antioxidant |
| topic | bovine serum albumin pullulan epigallocatechin gallate nanoparticles antioxidant activity |
| url | https://www.mdpi.com/2304-8158/11/24/4074 |
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