New Spirotetronate Antibiotics, Lobophorins H and I, from a South China Sea-Derived Streptomyces sp. 12A35
Strain 12A35 was isolated from a deep-sea sediment collected from the South China Sea and showed promising antibacterial activities. It was identified as Streptomyces sp. by the 16S rDNA sequence analysis. Bioassay-guided fractionation using HP20 adsorption, flash chromatography over silica gel and...
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| Format: | Article |
| Language: | English |
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MDPI AG
2013-10-01
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| Series: | Marine Drugs |
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| Online Access: | http://www.mdpi.com/1660-3397/11/10/3891 |
| _version_ | 1798005577013526528 |
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| author | Shu-Jin Wang Hui-Ming Hua Jiang-Chun Hu Zhan-Lin Li Nan Wang Song-Ya Zhang Hua-Qi Pan |
| author_facet | Shu-Jin Wang Hui-Ming Hua Jiang-Chun Hu Zhan-Lin Li Nan Wang Song-Ya Zhang Hua-Qi Pan |
| author_sort | Shu-Jin Wang |
| collection | DOAJ |
| description | Strain 12A35 was isolated from a deep-sea sediment collected from the South China Sea and showed promising antibacterial activities. It was identified as Streptomyces sp. by the 16S rDNA sequence analysis. Bioassay-guided fractionation using HP20 adsorption, flash chromatography over silica gel and octadecylsilyl (ODS) and semi-preparative HPLC, led to the isolation and purification of five metabolites from the fermentation culture of 12A35. Two new spirotetronate antibiotics, lobophorins H (1) and I (2), along with three known analogues, O-β-kijanosyl-(1→17)-kijanolide (3), lobophorins B (4) and F (5) were characterized by 1D, 2D-NMR and MS data. These compounds exhibited significant inhibitory activities against Bacillus subtilis. Compounds 1 and 5 exhibited moderate activities against Staphylococcus aureus. In particular, the new compound lobophorin H (1) showed similar antibacterial activities against B. subtilis CMCC63501 to ampicillin. |
| first_indexed | 2024-04-11T12:42:31Z |
| format | Article |
| id | doaj.art-d6598fb665e24b12bf1bad10477249a7 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T12:42:31Z |
| publishDate | 2013-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-d6598fb665e24b12bf1bad10477249a72022-12-22T04:23:28ZengMDPI AGMarine Drugs1660-33972013-10-0111103891390110.3390/md11103891New Spirotetronate Antibiotics, Lobophorins H and I, from a South China Sea-Derived Streptomyces sp. 12A35Shu-Jin WangHui-Ming HuaJiang-Chun HuZhan-Lin LiNan WangSong-Ya ZhangHua-Qi PanStrain 12A35 was isolated from a deep-sea sediment collected from the South China Sea and showed promising antibacterial activities. It was identified as Streptomyces sp. by the 16S rDNA sequence analysis. Bioassay-guided fractionation using HP20 adsorption, flash chromatography over silica gel and octadecylsilyl (ODS) and semi-preparative HPLC, led to the isolation and purification of five metabolites from the fermentation culture of 12A35. Two new spirotetronate antibiotics, lobophorins H (1) and I (2), along with three known analogues, O-β-kijanosyl-(1→17)-kijanolide (3), lobophorins B (4) and F (5) were characterized by 1D, 2D-NMR and MS data. These compounds exhibited significant inhibitory activities against Bacillus subtilis. Compounds 1 and 5 exhibited moderate activities against Staphylococcus aureus. In particular, the new compound lobophorin H (1) showed similar antibacterial activities against B. subtilis CMCC63501 to ampicillin.http://www.mdpi.com/1660-3397/11/10/3891lobophorinsspirotetronate antibioticsantimicrobialStreptomycesSouth China Sea-derived actinomycete |
| spellingShingle | Shu-Jin Wang Hui-Ming Hua Jiang-Chun Hu Zhan-Lin Li Nan Wang Song-Ya Zhang Hua-Qi Pan New Spirotetronate Antibiotics, Lobophorins H and I, from a South China Sea-Derived Streptomyces sp. 12A35 Marine Drugs lobophorins spirotetronate antibiotics antimicrobial Streptomyces South China Sea-derived actinomycete |
| title | New Spirotetronate Antibiotics, Lobophorins H and I, from a South China Sea-Derived Streptomyces sp. 12A35 |
| title_full | New Spirotetronate Antibiotics, Lobophorins H and I, from a South China Sea-Derived Streptomyces sp. 12A35 |
| title_fullStr | New Spirotetronate Antibiotics, Lobophorins H and I, from a South China Sea-Derived Streptomyces sp. 12A35 |
| title_full_unstemmed | New Spirotetronate Antibiotics, Lobophorins H and I, from a South China Sea-Derived Streptomyces sp. 12A35 |
| title_short | New Spirotetronate Antibiotics, Lobophorins H and I, from a South China Sea-Derived Streptomyces sp. 12A35 |
| title_sort | new spirotetronate antibiotics lobophorins h and i from a south china sea derived streptomyces sp 12a35 |
| topic | lobophorins spirotetronate antibiotics antimicrobial Streptomyces South China Sea-derived actinomycete |
| url | http://www.mdpi.com/1660-3397/11/10/3891 |
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