Antimicrobial activity of <i>Laminaria japonica</i> extracts against bacterial canker of tomato disease agent <i>Clavibacter michiganensis</i> subsp. <i>michiganensis</i>
Laminaria japonica was extracted with ethanol, and tested for antimicrobial activity against Clavibacter michiganensis subsp. michiganensis (Cmm). This gram-positive bacterium is the agent of a serious tomato disease called bacterial canker. On in vitro antimicrobial assays, L. japonica extracts sho...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Julius Kühn-Institut
2017-05-01
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| Series: | Journal of Applied Botany and Food Quality |
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| Online Access: | https://ojs.openagrar.de/index.php/JABFQ/article/view/7192 |
| _version_ | 1818415457413103616 |
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| author | Jin Cai Mengliang Wang Xiaoxia Zhao Yanyan Zhang Xin Yuan |
| author_facet | Jin Cai Mengliang Wang Xiaoxia Zhao Yanyan Zhang Xin Yuan |
| author_sort | Jin Cai |
| collection | DOAJ |
| description | Laminaria japonica was extracted with ethanol, and tested for antimicrobial activity against Clavibacter michiganensis subsp. michiganensis (Cmm). This gram-positive bacterium is the agent of a serious tomato disease called bacterial canker. On in vitro antimicrobial assays, L. japonica extracts showed intensive antimicrobial activity to inhibit Cmm. Extracting parameters (time, temperature, and solid/liquid ratio) were investigated and further optimized integratedly with L27(313) orthogonal array design (OAD). Our data implied that the optimum extraction conditions were A3B3C3, that were extracting time 12 h, extracting temperature 80 °C, and solid/liquid ratio 1:30 (g:mL). Extracting temperature was the significant factor affecting extracting condition. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of L. japonica extracts against Cmm were 2.5 mg/mL and 5 mg/mL. Scanning electron microscopy (SEM) observation indicated L. japonica extracts caused considerable morphological alterations in Cmm cells, such as protoplast leakage, cell membrane disruption, cell clumped, and cell fragmented. Reactive oxygen species (ROS) level determination and ATPase activity assay revealed that L. japonica extracts did cause significant accumulation in ROS and reduce the intracellular ATPase activity rapidly. Accordingly, these results indicated that L. japonica extracts could open a new promising opportunity for this tomato disease control. |
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| institution | Directory Open Access Journal |
| issn | 1613-9216 1439-040X |
| language | English |
| last_indexed | 2024-12-14T11:35:18Z |
| publishDate | 2017-05-01 |
| publisher | Julius Kühn-Institut |
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| series | Journal of Applied Botany and Food Quality |
| spelling | doaj.art-d65a39d3b1a34912a741f41542b6e93c2022-12-21T23:03:05ZengJulius Kühn-InstitutJournal of Applied Botany and Food Quality1613-92161439-040X2017-05-019010.5073/JABFQ.2017.090.0237192Antimicrobial activity of <i>Laminaria japonica</i> extracts against bacterial canker of tomato disease agent <i>Clavibacter michiganensis</i> subsp. <i>michiganensis</i>Jin Cai0Mengliang Wang1Xiaoxia Zhao2Yanyan Zhang3Xin Yuan4Institute of Applied Chemistry, Shanxi University, Taiyuan, Shanxi Province, People’s Republic of ChinaInstitute of Applied Chemistry, Shanxi University, Taiyuan, Shanxi Province, People’s Republic of ChinaInstitute of Applied Chemistry, Shanxi University, Taiyuan, Shanxi Province, People’s Republic of China1.Institute of Applied Chemistry, Shanxi University, Taiyuan, Shanxi Province, People’s Republic of China 2. Institute of Biotechnology, Shanxi University, Taiyuan, Shanxi Province, People’s Republic of China1Institute of Applied Chemistry, Shanxi University, Taiyuan, Shanxi Province, People’s Republic of China 2 Institute of Biotechnology, Shanxi University, Taiyuan, Shanxi Province, People’s Republic of ChinaLaminaria japonica was extracted with ethanol, and tested for antimicrobial activity against Clavibacter michiganensis subsp. michiganensis (Cmm). This gram-positive bacterium is the agent of a serious tomato disease called bacterial canker. On in vitro antimicrobial assays, L. japonica extracts showed intensive antimicrobial activity to inhibit Cmm. Extracting parameters (time, temperature, and solid/liquid ratio) were investigated and further optimized integratedly with L27(313) orthogonal array design (OAD). Our data implied that the optimum extraction conditions were A3B3C3, that were extracting time 12 h, extracting temperature 80 °C, and solid/liquid ratio 1:30 (g:mL). Extracting temperature was the significant factor affecting extracting condition. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of L. japonica extracts against Cmm were 2.5 mg/mL and 5 mg/mL. Scanning electron microscopy (SEM) observation indicated L. japonica extracts caused considerable morphological alterations in Cmm cells, such as protoplast leakage, cell membrane disruption, cell clumped, and cell fragmented. Reactive oxygen species (ROS) level determination and ATPase activity assay revealed that L. japonica extracts did cause significant accumulation in ROS and reduce the intracellular ATPase activity rapidly. Accordingly, these results indicated that L. japonica extracts could open a new promising opportunity for this tomato disease control.https://ojs.openagrar.de/index.php/JABFQ/article/view/7192bacterial canker of tomatoClavibacter michiganensis subsp. michiganensisLaminaria japonicaantimicrobial activityorthogonal array designantimicrobial mechanism |
| spellingShingle | Jin Cai Mengliang Wang Xiaoxia Zhao Yanyan Zhang Xin Yuan Antimicrobial activity of <i>Laminaria japonica</i> extracts against bacterial canker of tomato disease agent <i>Clavibacter michiganensis</i> subsp. <i>michiganensis</i> Journal of Applied Botany and Food Quality bacterial canker of tomato Clavibacter michiganensis subsp. michiganensis Laminaria japonica antimicrobial activity orthogonal array design antimicrobial mechanism |
| title | Antimicrobial activity of <i>Laminaria japonica</i> extracts against bacterial canker of tomato disease agent <i>Clavibacter michiganensis</i> subsp. <i>michiganensis</i> |
| title_full | Antimicrobial activity of <i>Laminaria japonica</i> extracts against bacterial canker of tomato disease agent <i>Clavibacter michiganensis</i> subsp. <i>michiganensis</i> |
| title_fullStr | Antimicrobial activity of <i>Laminaria japonica</i> extracts against bacterial canker of tomato disease agent <i>Clavibacter michiganensis</i> subsp. <i>michiganensis</i> |
| title_full_unstemmed | Antimicrobial activity of <i>Laminaria japonica</i> extracts against bacterial canker of tomato disease agent <i>Clavibacter michiganensis</i> subsp. <i>michiganensis</i> |
| title_short | Antimicrobial activity of <i>Laminaria japonica</i> extracts against bacterial canker of tomato disease agent <i>Clavibacter michiganensis</i> subsp. <i>michiganensis</i> |
| title_sort | antimicrobial activity of i laminaria japonica i extracts against bacterial canker of tomato disease agent i clavibacter michiganensis i subsp i michiganensis i |
| topic | bacterial canker of tomato Clavibacter michiganensis subsp. michiganensis Laminaria japonica antimicrobial activity orthogonal array design antimicrobial mechanism |
| url | https://ojs.openagrar.de/index.php/JABFQ/article/view/7192 |
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