Bacteroides fragilis strain ZY-312 promotes intestinal barrier integrity via upregulating the STAT3 pathway in a radiation-induced intestinal injury mouse model
Radiation-induced intestinal injury is characterized by intestinal barrier impairment. However, the therapeutic effects of probiotics for intestinal epithelial barrier repair in a mouse model of radiation-induced intestinal injury remain unclear. Previously, we isolated a strain of Bacteroides fragi...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-12-01
|
| Series: | Frontiers in Nutrition |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.1063699/full |
| _version_ | 1811296157954998272 |
|---|---|
| author | Qian Zhou Binhai Shen Ruo Huang Hongbin Liu Wendi Zhang Mengyao Song Ke Liu Xinlong Lin Shuze Chen Yangyang Liu Ye Wang Fachao Zhi |
| author_facet | Qian Zhou Binhai Shen Ruo Huang Hongbin Liu Wendi Zhang Mengyao Song Ke Liu Xinlong Lin Shuze Chen Yangyang Liu Ye Wang Fachao Zhi |
| author_sort | Qian Zhou |
| collection | DOAJ |
| description | Radiation-induced intestinal injury is characterized by intestinal barrier impairment. However, the therapeutic effects of probiotics for intestinal epithelial barrier repair in a mouse model of radiation-induced intestinal injury remain unclear. Previously, we isolated a strain of Bacteroides fragilis from the feces of a healthy infant and named it as B. fragilis strain ZY-312 (B. fragilis). In this study, we showed that B. fragilis can ameliorate radiation-induced intestinal injury in mice, manifested by decreased weight loss, intestinal length shortening, and intestinal epithelial cell (IEC) shedding. Moreover, we found that B. fragilis promoted IEC proliferation, stem cell regeneration, mucus secretion, and tight junction integrity by upregulating the STAT3 signaling pathway, through an experimental verification in Stat3△IEC mice (STAT3 defects in intestinal epithelial cells). Thus, the underlying protective mechanism of B. fragilis in radiation-induced intestinal injury is related to IEC proliferation, stem cell regeneration, goblet cell secretion, and tight junction repair via activation of the STAT3 signaling pathway. In addition, the therapeutic effects of B. fragilis were studied to provide new insights into its application as a functional and clinical drug for radiation-induced intestinal injury after radiotherapy. |
| first_indexed | 2024-04-13T05:44:23Z |
| format | Article |
| id | doaj.art-d65c550a0ede40fc8c0b223433cbc3e5 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-04-13T05:44:23Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-d65c550a0ede40fc8c0b223433cbc3e52022-12-22T03:00:00ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-12-01910.3389/fnut.2022.10636991063699Bacteroides fragilis strain ZY-312 promotes intestinal barrier integrity via upregulating the STAT3 pathway in a radiation-induced intestinal injury mouse modelQian Zhou0Binhai Shen1Ruo Huang2Hongbin Liu3Wendi Zhang4Mengyao Song5Ke Liu6Xinlong Lin7Shuze Chen8Yangyang Liu9Ye Wang10Fachao Zhi11Department of Gastroenterology, Institute of Gastroenterology of Guangdong Province, Guangdong Provincial Key Laboratory of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Gastroenterology, Institute of Gastroenterology of Guangdong Province, Guangdong Provincial Key Laboratory of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Gastroenterology, Institute of Gastroenterology of Guangdong Province, Guangdong Provincial Key Laboratory of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Gastroenterology, Institute of Gastroenterology of Guangdong Province, Guangdong Provincial Key Laboratory of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Gastroenterology, Institute of Gastroenterology of Guangdong Province, Guangdong Provincial Key Laboratory of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Gastroenterology, Institute of Gastroenterology of Guangdong Province, Guangdong Provincial Key Laboratory of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Gastroenterology, Institute of Gastroenterology of Guangdong Province, Guangdong Provincial Key Laboratory of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Gastroenterology, Institute of Gastroenterology of Guangdong Province, Guangdong Provincial Key Laboratory of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Gastroenterology, Institute of Gastroenterology of Guangdong Province, Guangdong Provincial Key Laboratory of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaGuangzhou Zhiyi Biotechnology Co., Ltd., Guangzhou, ChinaGuangzhou Zhiyi Biotechnology Co., Ltd., Guangzhou, ChinaDepartment of Gastroenterology, Institute of Gastroenterology of Guangdong Province, Guangdong Provincial Key Laboratory of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaRadiation-induced intestinal injury is characterized by intestinal barrier impairment. However, the therapeutic effects of probiotics for intestinal epithelial barrier repair in a mouse model of radiation-induced intestinal injury remain unclear. Previously, we isolated a strain of Bacteroides fragilis from the feces of a healthy infant and named it as B. fragilis strain ZY-312 (B. fragilis). In this study, we showed that B. fragilis can ameliorate radiation-induced intestinal injury in mice, manifested by decreased weight loss, intestinal length shortening, and intestinal epithelial cell (IEC) shedding. Moreover, we found that B. fragilis promoted IEC proliferation, stem cell regeneration, mucus secretion, and tight junction integrity by upregulating the STAT3 signaling pathway, through an experimental verification in Stat3△IEC mice (STAT3 defects in intestinal epithelial cells). Thus, the underlying protective mechanism of B. fragilis in radiation-induced intestinal injury is related to IEC proliferation, stem cell regeneration, goblet cell secretion, and tight junction repair via activation of the STAT3 signaling pathway. In addition, the therapeutic effects of B. fragilis were studied to provide new insights into its application as a functional and clinical drug for radiation-induced intestinal injury after radiotherapy.https://www.frontiersin.org/articles/10.3389/fnut.2022.1063699/fullBacteroides fragilis strain ZY-312probioticsradiation-induced intestinal injurystem cellsgoblet cellstight junction |
| spellingShingle | Qian Zhou Binhai Shen Ruo Huang Hongbin Liu Wendi Zhang Mengyao Song Ke Liu Xinlong Lin Shuze Chen Yangyang Liu Ye Wang Fachao Zhi Bacteroides fragilis strain ZY-312 promotes intestinal barrier integrity via upregulating the STAT3 pathway in a radiation-induced intestinal injury mouse model Frontiers in Nutrition Bacteroides fragilis strain ZY-312 probiotics radiation-induced intestinal injury stem cells goblet cells tight junction |
| title | Bacteroides fragilis strain ZY-312 promotes intestinal barrier integrity via upregulating the STAT3 pathway in a radiation-induced intestinal injury mouse model |
| title_full | Bacteroides fragilis strain ZY-312 promotes intestinal barrier integrity via upregulating the STAT3 pathway in a radiation-induced intestinal injury mouse model |
| title_fullStr | Bacteroides fragilis strain ZY-312 promotes intestinal barrier integrity via upregulating the STAT3 pathway in a radiation-induced intestinal injury mouse model |
| title_full_unstemmed | Bacteroides fragilis strain ZY-312 promotes intestinal barrier integrity via upregulating the STAT3 pathway in a radiation-induced intestinal injury mouse model |
| title_short | Bacteroides fragilis strain ZY-312 promotes intestinal barrier integrity via upregulating the STAT3 pathway in a radiation-induced intestinal injury mouse model |
| title_sort | bacteroides fragilis strain zy 312 promotes intestinal barrier integrity via upregulating the stat3 pathway in a radiation induced intestinal injury mouse model |
| topic | Bacteroides fragilis strain ZY-312 probiotics radiation-induced intestinal injury stem cells goblet cells tight junction |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.1063699/full |
| work_keys_str_mv | AT qianzhou bacteroidesfragilisstrainzy312promotesintestinalbarrierintegrityviaupregulatingthestat3pathwayinaradiationinducedintestinalinjurymousemodel AT binhaishen bacteroidesfragilisstrainzy312promotesintestinalbarrierintegrityviaupregulatingthestat3pathwayinaradiationinducedintestinalinjurymousemodel AT ruohuang bacteroidesfragilisstrainzy312promotesintestinalbarrierintegrityviaupregulatingthestat3pathwayinaradiationinducedintestinalinjurymousemodel AT hongbinliu bacteroidesfragilisstrainzy312promotesintestinalbarrierintegrityviaupregulatingthestat3pathwayinaradiationinducedintestinalinjurymousemodel AT wendizhang bacteroidesfragilisstrainzy312promotesintestinalbarrierintegrityviaupregulatingthestat3pathwayinaradiationinducedintestinalinjurymousemodel AT mengyaosong bacteroidesfragilisstrainzy312promotesintestinalbarrierintegrityviaupregulatingthestat3pathwayinaradiationinducedintestinalinjurymousemodel AT keliu bacteroidesfragilisstrainzy312promotesintestinalbarrierintegrityviaupregulatingthestat3pathwayinaradiationinducedintestinalinjurymousemodel AT xinlonglin bacteroidesfragilisstrainzy312promotesintestinalbarrierintegrityviaupregulatingthestat3pathwayinaradiationinducedintestinalinjurymousemodel AT shuzechen bacteroidesfragilisstrainzy312promotesintestinalbarrierintegrityviaupregulatingthestat3pathwayinaradiationinducedintestinalinjurymousemodel AT yangyangliu bacteroidesfragilisstrainzy312promotesintestinalbarrierintegrityviaupregulatingthestat3pathwayinaradiationinducedintestinalinjurymousemodel AT yewang bacteroidesfragilisstrainzy312promotesintestinalbarrierintegrityviaupregulatingthestat3pathwayinaradiationinducedintestinalinjurymousemodel AT fachaozhi bacteroidesfragilisstrainzy312promotesintestinalbarrierintegrityviaupregulatingthestat3pathwayinaradiationinducedintestinalinjurymousemodel |