Effectiveness of immunization with multi-component bacterial immunomodulator in foals at 35th day of life
Abstract The aim of the study was to investigate the mechanisms leading to immunization through the use of a multicomponent bacterial immunomodulator and to find out the relationship between the TLR 4 receptor with selected parameters of innate immunity and to acquire immunity. The study was conduct...
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| Format: | Article |
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Nature Portfolio
2022-09-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-17532-1 |
| _version_ | 1828111671921475584 |
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| author | Migdał Anna Migdał Łukasz Okólski Adam Anna Chełmońska-Soyta |
| author_facet | Migdał Anna Migdał Łukasz Okólski Adam Anna Chełmońska-Soyta |
| author_sort | Migdał Anna |
| collection | DOAJ |
| description | Abstract The aim of the study was to investigate the mechanisms leading to immunization through the use of a multicomponent bacterial immunomodulator and to find out the relationship between the TLR 4 receptor with selected parameters of innate immunity and to acquire immunity. The study was conducted on 18 Polish Pony Horses foals divided into two study groups: control (n = 9) and experimental (n = 9). Foals from the experimental group received intramuscular duplicate injection of 5 ml of multi-component bacterial immunomodular at 35 and 40 days of age. RNA isolated from venous blood was used to evaluate the expression of TLR4 genes using RT-PCR. Concentration of Il-6, IL-10, IgM and IgG2 was determined by the ELISA method in blood plasma. Immunostimulation had a significant impact on the level of genes expression for TLR4 expression and IL-6 concentration. No effect of stimulation on IgM and IgG2 concentrations was found. The expression of TLR4 genes as well as the levels of interleukins could be modulated by stimulation with a pharmacological agent multi-component bacterial immunomodulator. The experiment demonstrated a strong positive correlation between TLR4 gene expression and IL-6 concentration and between TLR4 gene expression and IgM concentration. |
| first_indexed | 2024-04-11T11:38:22Z |
| format | Article |
| id | doaj.art-d65e801b23434161a1e8fafdbfa39f66 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T11:38:22Z |
| publishDate | 2022-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-d65e801b23434161a1e8fafdbfa39f662022-12-22T04:25:53ZengNature PortfolioScientific Reports2045-23222022-09-011211910.1038/s41598-022-17532-1Effectiveness of immunization with multi-component bacterial immunomodulator in foals at 35th day of lifeMigdał Anna0Migdał Łukasz1Okólski Adam2Anna Chełmońska-Soyta3Department of Genetics, Animal Breeding and Ethology, Faculty of Animal Sciences, University of Agriculture in KrakowDepartment of Genetics, Animal Breeding and Ethology, Faculty of Animal Sciences, University of Agriculture in KrakowUniversity Centre of Veterinary Medicine UJ-UR, University of Agriculture in KrakowLaboratory of Reproductive Immunology, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of SciencesAbstract The aim of the study was to investigate the mechanisms leading to immunization through the use of a multicomponent bacterial immunomodulator and to find out the relationship between the TLR 4 receptor with selected parameters of innate immunity and to acquire immunity. The study was conducted on 18 Polish Pony Horses foals divided into two study groups: control (n = 9) and experimental (n = 9). Foals from the experimental group received intramuscular duplicate injection of 5 ml of multi-component bacterial immunomodular at 35 and 40 days of age. RNA isolated from venous blood was used to evaluate the expression of TLR4 genes using RT-PCR. Concentration of Il-6, IL-10, IgM and IgG2 was determined by the ELISA method in blood plasma. Immunostimulation had a significant impact on the level of genes expression for TLR4 expression and IL-6 concentration. No effect of stimulation on IgM and IgG2 concentrations was found. The expression of TLR4 genes as well as the levels of interleukins could be modulated by stimulation with a pharmacological agent multi-component bacterial immunomodulator. The experiment demonstrated a strong positive correlation between TLR4 gene expression and IL-6 concentration and between TLR4 gene expression and IgM concentration.https://doi.org/10.1038/s41598-022-17532-1 |
| spellingShingle | Migdał Anna Migdał Łukasz Okólski Adam Anna Chełmońska-Soyta Effectiveness of immunization with multi-component bacterial immunomodulator in foals at 35th day of life Scientific Reports |
| title | Effectiveness of immunization with multi-component bacterial immunomodulator in foals at 35th day of life |
| title_full | Effectiveness of immunization with multi-component bacterial immunomodulator in foals at 35th day of life |
| title_fullStr | Effectiveness of immunization with multi-component bacterial immunomodulator in foals at 35th day of life |
| title_full_unstemmed | Effectiveness of immunization with multi-component bacterial immunomodulator in foals at 35th day of life |
| title_short | Effectiveness of immunization with multi-component bacterial immunomodulator in foals at 35th day of life |
| title_sort | effectiveness of immunization with multi component bacterial immunomodulator in foals at 35th day of life |
| url | https://doi.org/10.1038/s41598-022-17532-1 |
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