Detection of fowl poxvirus integrated with reticuloendotheliosis virus sequences from an outbreak in backyard chickens in India
Fowl poxvirus (FPV) infection was observed in unvaccinated backyard chickens. A total of 15 birds were affected in a flock of 37. Pock lesions were observed on the comb, eyelids, beak and wattles. The birds appeared sick with roughened feathers and stunted growth. No mortality was recorded. DNA was...
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| Format: | Article |
| Language: | English |
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Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale
2011-06-01
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| Series: | Veterinaria Italiana |
| Subjects: | |
| Online Access: | http://www.izs.it/vet_italiana/2011/47_2/147.pdf |
| _version_ | 1811202112961380352 |
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| author | Sanchay K. Biswas Chandrakanta Jana Karam Chand Waseem Rehman Bimalendu Mondal |
| author_facet | Sanchay K. Biswas Chandrakanta Jana Karam Chand Waseem Rehman Bimalendu Mondal |
| author_sort | Sanchay K. Biswas |
| collection | DOAJ |
| description | Fowl poxvirus (FPV) infection was observed in unvaccinated backyard chickens. A total of 15 birds were affected in a flock of 37. Pock lesions were observed on the comb, eyelids, beak and wattles. The birds appeared sick with roughened feathers and stunted growth. No mortality was recorded. DNA was isolated from scabs and polymerase chain reaction (PCR) was performed to amplify the 4b core protein gene of FPV, the envelope (env) gene of reticuloendotheliosis virus (REV) and the region of FPV flanking REV 5´ long terminal repeat (LTR). Correct-size PCR products of 578 bp, 807 bp and 370 bp, respectively, were observed in agarose gel electrophoresis. Sequence analysis of these products suggests that the virus was an FPV with a genome containing an integrated near full-length REV provirus. Given the fact that REV has been associated with immunosuppression, its presence in the genome of FPV appears to play an important role in the pathogenesis of fowl pox and presumably prolongs persistence of FPV in bird populations. In the present case, fowl pox has been observed to have persisted for about three years in fowl that were reared in backyard systems in villages. |
| first_indexed | 2024-04-12T02:33:32Z |
| format | Article |
| id | doaj.art-d66046c7d04e443f8a956fa6366f9192 |
| institution | Directory Open Access Journal |
| issn | 0505-401X 1828-1427 |
| language | English |
| last_indexed | 2024-04-12T02:33:32Z |
| publishDate | 2011-06-01 |
| publisher | Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale |
| record_format | Article |
| series | Veterinaria Italiana |
| spelling | doaj.art-d66046c7d04e443f8a956fa6366f91922022-12-22T03:51:38ZengIstituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. CaporaleVeterinaria Italiana0505-401X1828-14272011-06-01472147153Detection of fowl poxvirus integrated with reticuloendotheliosis virus sequences from an outbreak in backyard chickens in IndiaSanchay K. BiswasChandrakanta JanaKaram ChandWaseem RehmanBimalendu MondalFowl poxvirus (FPV) infection was observed in unvaccinated backyard chickens. A total of 15 birds were affected in a flock of 37. Pock lesions were observed on the comb, eyelids, beak and wattles. The birds appeared sick with roughened feathers and stunted growth. No mortality was recorded. DNA was isolated from scabs and polymerase chain reaction (PCR) was performed to amplify the 4b core protein gene of FPV, the envelope (env) gene of reticuloendotheliosis virus (REV) and the region of FPV flanking REV 5´ long terminal repeat (LTR). Correct-size PCR products of 578 bp, 807 bp and 370 bp, respectively, were observed in agarose gel electrophoresis. Sequence analysis of these products suggests that the virus was an FPV with a genome containing an integrated near full-length REV provirus. Given the fact that REV has been associated with immunosuppression, its presence in the genome of FPV appears to play an important role in the pathogenesis of fowl pox and presumably prolongs persistence of FPV in bird populations. In the present case, fowl pox has been observed to have persisted for about three years in fowl that were reared in backyard systems in villages.http://www.izs.it/vet_italiana/2011/47_2/147.pdfChickenFowlIndiaPolymerase chain reactionPCRPoxvirusRecurring infectionReticuloendotheliosis virusREVVirus. |
| spellingShingle | Sanchay K. Biswas Chandrakanta Jana Karam Chand Waseem Rehman Bimalendu Mondal Detection of fowl poxvirus integrated with reticuloendotheliosis virus sequences from an outbreak in backyard chickens in India Veterinaria Italiana Chicken Fowl India Polymerase chain reaction PCR Poxvirus Recurring infection Reticuloendotheliosis virus REV Virus. |
| title | Detection of fowl poxvirus integrated with reticuloendotheliosis virus sequences from an outbreak in backyard chickens in India |
| title_full | Detection of fowl poxvirus integrated with reticuloendotheliosis virus sequences from an outbreak in backyard chickens in India |
| title_fullStr | Detection of fowl poxvirus integrated with reticuloendotheliosis virus sequences from an outbreak in backyard chickens in India |
| title_full_unstemmed | Detection of fowl poxvirus integrated with reticuloendotheliosis virus sequences from an outbreak in backyard chickens in India |
| title_short | Detection of fowl poxvirus integrated with reticuloendotheliosis virus sequences from an outbreak in backyard chickens in India |
| title_sort | detection of fowl poxvirus integrated with reticuloendotheliosis virus sequences from an outbreak in backyard chickens in india |
| topic | Chicken Fowl India Polymerase chain reaction PCR Poxvirus Recurring infection Reticuloendotheliosis virus REV Virus. |
| url | http://www.izs.it/vet_italiana/2011/47_2/147.pdf |
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