Introduction and multiplication in vitro of garlic culture var. Morado Barranquino
We determine the effect of modified culture medium using different growth regulators concentrations (auxins / cytokinins) for regeneration and propagation of in vitro garlic plants (Allium sativumL.) var. ‘Morado Barrran-quino’. In the introduction stage meristem tips were cultured in test tubes up...
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| Format: | Article |
| Language: | English |
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Universidad Nacional Mayor de San Marcos
2013-06-01
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| Series: | Revista Peruana de Biología |
| Subjects: | |
| Online Access: | http://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/1051 |
| _version_ | 1811234977517404160 |
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| author | Karina Carhuaricra E. Julio Olivera S. Javier Gonzales A. Juan Rodríguez L. |
| author_facet | Karina Carhuaricra E. Julio Olivera S. Javier Gonzales A. Juan Rodríguez L. |
| author_sort | Karina Carhuaricra E. |
| collection | DOAJ |
| description | We determine the effect of modified culture medium using different growth regulators concentrations (auxins / cytokinins) for regeneration and propagation of in vitro garlic plants (Allium sativumL.) var. ‘Morado Barrran-quino’. In the introduction stage meristem tips were cultured in test tubes up to regeneration. It was determined that medium MSM + IBA 0.6 mg/L was most suitable for the meristem tip differentiation and development. 13 weeks old microplants obtained on introduction stage were used for multiplication, obtaining the formation of a cluster after each subcultive. The best multiplication ratio was obtained in MMA medium + IAA 2.5 mg/L + KIN 5.0 mg/L and MSM medium + 2iP 1.5 mg/L with 4.71 and 4.79 respectively. |
| first_indexed | 2024-04-12T11:44:24Z |
| format | Article |
| id | doaj.art-d6641ad1ac394bfd9d663bc7119862e7 |
| institution | Directory Open Access Journal |
| issn | 1561-0837 1727-9933 |
| language | English |
| last_indexed | 2024-04-12T11:44:24Z |
| publishDate | 2013-06-01 |
| publisher | Universidad Nacional Mayor de San Marcos |
| record_format | Article |
| series | Revista Peruana de Biología |
| spelling | doaj.art-d6641ad1ac394bfd9d663bc7119862e72022-12-22T03:34:27ZengUniversidad Nacional Mayor de San MarcosRevista Peruana de Biología1561-08371727-99332013-06-0119334134410.15381/rpb.v19i3.1051920Introduction and multiplication in vitro of garlic culture var. Morado BarranquinoKarina Carhuaricra E.0Julio Olivera S.1Javier Gonzales A.2Juan Rodríguez L.3Universidad Nacional Daniel Alcides Carrión - Facultad de Ciencias Agropecuarias. Ciudad Universitaria km 3 Carretera Cen-tral Miraflores – OxapampaEstación Experimental Agraria DONOSO -Instituto Nacional de Innovación Agraria. Altura km 5,4 de la Carretera Chancay – HuaralUniversidad Nacional Daniel Alcides Carrión - Facultad de Ciencias Agropecuarias. Ciudad Universitaria km 3 Carretera Cen-tral Miraflores – Oxapampa.Universidad Nacional Daniel Alcides Carrión - Facultad de Ciencias Agropecuarias. Ciudad Universitaria km 3 Carretera Cen-tral Miraflores – Oxapampa.We determine the effect of modified culture medium using different growth regulators concentrations (auxins / cytokinins) for regeneration and propagation of in vitro garlic plants (Allium sativumL.) var. ‘Morado Barrran-quino’. In the introduction stage meristem tips were cultured in test tubes up to regeneration. It was determined that medium MSM + IBA 0.6 mg/L was most suitable for the meristem tip differentiation and development. 13 weeks old microplants obtained on introduction stage were used for multiplication, obtaining the formation of a cluster after each subcultive. The best multiplication ratio was obtained in MMA medium + IAA 2.5 mg/L + KIN 5.0 mg/L and MSM medium + 2iP 1.5 mg/L with 4.71 and 4.79 respectively.http://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/1051Allium sativumexplantein vitromeristemo caulinarsubcultivo |
| spellingShingle | Karina Carhuaricra E. Julio Olivera S. Javier Gonzales A. Juan Rodríguez L. Introduction and multiplication in vitro of garlic culture var. Morado Barranquino Revista Peruana de Biología Allium sativum explante in vitro meristemo caulinar subcultivo |
| title | Introduction and multiplication in vitro of garlic culture var. Morado Barranquino |
| title_full | Introduction and multiplication in vitro of garlic culture var. Morado Barranquino |
| title_fullStr | Introduction and multiplication in vitro of garlic culture var. Morado Barranquino |
| title_full_unstemmed | Introduction and multiplication in vitro of garlic culture var. Morado Barranquino |
| title_short | Introduction and multiplication in vitro of garlic culture var. Morado Barranquino |
| title_sort | introduction and multiplication in vitro of garlic culture var morado barranquino |
| topic | Allium sativum explante in vitro meristemo caulinar subcultivo |
| url | http://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/1051 |
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