Antimicrobial Susceptibility and Virulence Surveillance of Campylobacter spp. Isolated From Patients in Two Tertiary Medical Centers in Taiwan
Campylobacter spp. may cause fever, vomiting, and diarrhea in humans. Antibiotic treatment is suggested for patients with severe campylobacteriosis. However, the interpretative criteria for antibiotic susceptibility are inconsistent between Clinical and Laboratory Standards Institute and European Co...
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Frontiers Media S.A.
2019-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2018.03186/full |
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| author | Mao-Cheng Ge Mao-Cheng Ge Shu-Fang Kuo Shu-Fang Kuo Shih-Cheng Chang Shih-Cheng Chang Chun-Chih Chien Huey-Ling You Jang-Jih Lu Jang-Jih Lu Jang-Jih Lu |
| author_facet | Mao-Cheng Ge Mao-Cheng Ge Shu-Fang Kuo Shu-Fang Kuo Shih-Cheng Chang Shih-Cheng Chang Chun-Chih Chien Huey-Ling You Jang-Jih Lu Jang-Jih Lu Jang-Jih Lu |
| author_sort | Mao-Cheng Ge |
| collection | DOAJ |
| description | Campylobacter spp. may cause fever, vomiting, and diarrhea in humans. Antibiotic treatment is suggested for patients with severe campylobacteriosis. However, the interpretative criteria for antibiotic susceptibility are inconsistent between Clinical and Laboratory Standards Institute and European Committee on Antimicrobial Susceptibility Testing guidelines. The aim of the study is to investigate the antibiotic susceptibility and prevalence of cytolethal distending toxin genes and to evaluate antibiotic susceptibility testing methods in the clinical laboratories of two tertiary medical centers in Taiwan. In total, 236 bacterial isolates were collected between 2001 and 2014. The disk diffusion and E-test methods were used to evaluate the antibiotic susceptibility, and broth dilution results were used as a reference. The virulence genes cdtA, cdtB, cdtC, and ceuE were detected through polymerase chain reaction. The antimicrobial sensitivity rates for erythromycin, ciprofloxacin, and tetracycline using the broth dilution assay were 80.4, 5.4, and 3.4%, respectively. No significant differences were observed in the antibiotic susceptibility of the isolates obtained from southern and northern Taiwan. However, some differences were observed between species. The susceptibility test for erythromycin (disk diffusion) showed that the isolates with small inhibition zone diameters were all resistant, and five isolates (4.0%) with large IZDs were non-sensitive. The error rate of the disk diffusion method according to the CLSI M45-A3 guideline was 5.4% (8/148). The incompatibility rates between the E-test and broth dilution methods for erythromycin, ciprofloxacin, and tetracycline were 8.0, 5.3, and 1.3%, respectively. The positive rates of the genes cdtA and cdtC were considerably higher in Campylobacter jejuni than in C. coli. Erythromycin is recommended as the first choice of treatment for campylobacteriosis. The disk diffusion method is suitable for prescreening Campylobacter susceptibility by using the CLSI M45-A2 and EUCAST criteria (low error rate of 3.4%). If antibiotic treatment fails or IZDs are between 6 and 20 mm, minimum inhibitory concentration testing by using the E-test method is highly recommended because the results of the E-test and broth dilution methods exhibit high agreement. The error rate of disk diffusion method using CLSI M45-A3 criteria is only slightly higher than B, which is also a suitable criteria. |
| first_indexed | 2024-12-22T09:37:04Z |
| format | Article |
| id | doaj.art-d6679a3bbd544aea827d581aa22d3858 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-22T09:37:04Z |
| publishDate | 2019-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-d6679a3bbd544aea827d581aa22d38582022-12-21T18:30:48ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-01-01910.3389/fmicb.2018.03186415752Antimicrobial Susceptibility and Virulence Surveillance of Campylobacter spp. Isolated From Patients in Two Tertiary Medical Centers in TaiwanMao-Cheng Ge0Mao-Cheng Ge1Shu-Fang Kuo2Shu-Fang Kuo3Shih-Cheng Chang4Shih-Cheng Chang5Chun-Chih Chien6Huey-Ling You7Jang-Jih Lu8Jang-Jih Lu9Jang-Jih Lu10Department of Laboratory Medicine, Chang Gung Memorial Hospital, Taoyuan, TaiwanDepartment of Medical Biotechnology and Laboratory Sciences, College of Medicine, Chang Gung University, Taoyuan, TaiwanDepartment of Medical Biotechnology and Laboratory Sciences, College of Medicine, Chang Gung University, Taoyuan, TaiwanDepartment of Laboratory Medicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, TaiwanDepartment of Laboratory Medicine, Chang Gung Memorial Hospital, Taoyuan, TaiwanDepartment of Medical Biotechnology and Laboratory Sciences, College of Medicine, Chang Gung University, Taoyuan, TaiwanDepartment of Laboratory Medicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, TaiwanDepartment of Laboratory Medicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, TaiwanDepartment of Laboratory Medicine, Chang Gung Memorial Hospital, Taoyuan, TaiwanDepartment of Medical Biotechnology and Laboratory Sciences, College of Medicine, Chang Gung University, Taoyuan, TaiwanDepartment of Medicine, College of Medicine, Chang Gung University, Taoyuan, TaiwanCampylobacter spp. may cause fever, vomiting, and diarrhea in humans. Antibiotic treatment is suggested for patients with severe campylobacteriosis. However, the interpretative criteria for antibiotic susceptibility are inconsistent between Clinical and Laboratory Standards Institute and European Committee on Antimicrobial Susceptibility Testing guidelines. The aim of the study is to investigate the antibiotic susceptibility and prevalence of cytolethal distending toxin genes and to evaluate antibiotic susceptibility testing methods in the clinical laboratories of two tertiary medical centers in Taiwan. In total, 236 bacterial isolates were collected between 2001 and 2014. The disk diffusion and E-test methods were used to evaluate the antibiotic susceptibility, and broth dilution results were used as a reference. The virulence genes cdtA, cdtB, cdtC, and ceuE were detected through polymerase chain reaction. The antimicrobial sensitivity rates for erythromycin, ciprofloxacin, and tetracycline using the broth dilution assay were 80.4, 5.4, and 3.4%, respectively. No significant differences were observed in the antibiotic susceptibility of the isolates obtained from southern and northern Taiwan. However, some differences were observed between species. The susceptibility test for erythromycin (disk diffusion) showed that the isolates with small inhibition zone diameters were all resistant, and five isolates (4.0%) with large IZDs were non-sensitive. The error rate of the disk diffusion method according to the CLSI M45-A3 guideline was 5.4% (8/148). The incompatibility rates between the E-test and broth dilution methods for erythromycin, ciprofloxacin, and tetracycline were 8.0, 5.3, and 1.3%, respectively. The positive rates of the genes cdtA and cdtC were considerably higher in Campylobacter jejuni than in C. coli. Erythromycin is recommended as the first choice of treatment for campylobacteriosis. The disk diffusion method is suitable for prescreening Campylobacter susceptibility by using the CLSI M45-A2 and EUCAST criteria (low error rate of 3.4%). If antibiotic treatment fails or IZDs are between 6 and 20 mm, minimum inhibitory concentration testing by using the E-test method is highly recommended because the results of the E-test and broth dilution methods exhibit high agreement. The error rate of disk diffusion method using CLSI M45-A3 criteria is only slightly higher than B, which is also a suitable criteria.https://www.frontiersin.org/article/10.3389/fmicb.2018.03186/fullCampylobacterantibiotic susceptibilityvirulence factordisk diffusionE-test |
| spellingShingle | Mao-Cheng Ge Mao-Cheng Ge Shu-Fang Kuo Shu-Fang Kuo Shih-Cheng Chang Shih-Cheng Chang Chun-Chih Chien Huey-Ling You Jang-Jih Lu Jang-Jih Lu Jang-Jih Lu Antimicrobial Susceptibility and Virulence Surveillance of Campylobacter spp. Isolated From Patients in Two Tertiary Medical Centers in Taiwan Frontiers in Microbiology Campylobacter antibiotic susceptibility virulence factor disk diffusion E-test |
| title | Antimicrobial Susceptibility and Virulence Surveillance of Campylobacter spp. Isolated From Patients in Two Tertiary Medical Centers in Taiwan |
| title_full | Antimicrobial Susceptibility and Virulence Surveillance of Campylobacter spp. Isolated From Patients in Two Tertiary Medical Centers in Taiwan |
| title_fullStr | Antimicrobial Susceptibility and Virulence Surveillance of Campylobacter spp. Isolated From Patients in Two Tertiary Medical Centers in Taiwan |
| title_full_unstemmed | Antimicrobial Susceptibility and Virulence Surveillance of Campylobacter spp. Isolated From Patients in Two Tertiary Medical Centers in Taiwan |
| title_short | Antimicrobial Susceptibility and Virulence Surveillance of Campylobacter spp. Isolated From Patients in Two Tertiary Medical Centers in Taiwan |
| title_sort | antimicrobial susceptibility and virulence surveillance of campylobacter spp isolated from patients in two tertiary medical centers in taiwan |
| topic | Campylobacter antibiotic susceptibility virulence factor disk diffusion E-test |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2018.03186/full |
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