Metagenomics Reveals Dominant Unusual Sulfur Oxidizers Inhabiting Active Hydrothermal Chimneys From the Southwest Indian Ridge
The deep-sea hydrothermal vents (DSHVs) in the Southwest Indian Ridge (SWIR) are formed by specific geological settings. However, the community structure and ecological function of the microbial inhabitants on the sulfide chimneys of active hydrothermal vents remain largely unknown. In this study, o...
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.861795/full |
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| author | Yong Wang Hong-Yu Bi Hua-Guan Chen Hua-Guan Chen Peng-Fei Zheng Ying-Li Zhou Ying-Li Zhou Jiang-Tao Li |
| author_facet | Yong Wang Hong-Yu Bi Hua-Guan Chen Hua-Guan Chen Peng-Fei Zheng Ying-Li Zhou Ying-Li Zhou Jiang-Tao Li |
| author_sort | Yong Wang |
| collection | DOAJ |
| description | The deep-sea hydrothermal vents (DSHVs) in the Southwest Indian Ridge (SWIR) are formed by specific geological settings. However, the community structure and ecological function of the microbial inhabitants on the sulfide chimneys of active hydrothermal vents remain largely unknown. In this study, our analyses of 16S rRNA gene amplicons and 16S rRNA metagenomic reads showed the dominance of sulfur-oxidizing Ectothiorhodospiraceae, Thiomicrorhabdus, Sulfurimonas, and Sulfurovum on the wall of two active hydrothermal chimneys. Compared with the inactive hydrothermal sediments of SWIR, the active hydrothermal chimneys lacked sulfur-reducing bacteria. The metabolic potentials of the retrieved 82 metagenome-assembled genomes (MAGs) suggest that sulfur oxidation might be conducted by Thiohalomonadales (classified as Ectothiorhodospiraceae based on 16S rRNA gene amplicons), Sulfurovaceae, Hyphomicrobiaceae, Thiotrichaceae, Thiomicrospiraceae, and Rhodobacteraceae. For CO2 fixation, the Calvin-Benson-Bassham and reductive TCA pathways were employed by these bacteria. In Thiohalomonadales MAGs, we revealed putative phytochrome, carotenoid precursor, and squalene synthesis pathways, indicating a possible capacity of Thiohalomonadales in adaptation to dynamics redox conditions and the utilization of red light from the hot hydrothermal chimneys for photolithotrophic growth. This study, therefore, reveals unique microbiomes and their genomic features in the active hydrothermal chimneys of SWIR, which casts light on ecosystem establishment and development in hydrothermal fields and the deep biosphere. |
| first_indexed | 2024-04-14T00:13:44Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-14T00:13:44Z |
| publishDate | 2022-05-01 |
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| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-d668ea6edf384904a42a418862c658032022-12-22T02:23:13ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-05-011310.3389/fmicb.2022.861795861795Metagenomics Reveals Dominant Unusual Sulfur Oxidizers Inhabiting Active Hydrothermal Chimneys From the Southwest Indian RidgeYong Wang0Hong-Yu Bi1Hua-Guan Chen2Hua-Guan Chen3Peng-Fei Zheng4Ying-Li Zhou5Ying-Li Zhou6Jiang-Tao Li7Institute for Marine Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen, ChinaInstitute of Deep Sea Science and Engineering, Chinese Academy of Sciences, Sanya, ChinaInstitute of Deep Sea Science and Engineering, Chinese Academy of Sciences, Sanya, ChinaCollege of Marine Sciences, University of Chinese Academy of Sciences, Beijing, ChinaInstitute of Deep Sea Science and Engineering, Chinese Academy of Sciences, Sanya, ChinaInstitute of Deep Sea Science and Engineering, Chinese Academy of Sciences, Sanya, ChinaCollege of Marine Sciences, University of Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Marine Geology, Tongji University, Shanghai, ChinaThe deep-sea hydrothermal vents (DSHVs) in the Southwest Indian Ridge (SWIR) are formed by specific geological settings. However, the community structure and ecological function of the microbial inhabitants on the sulfide chimneys of active hydrothermal vents remain largely unknown. In this study, our analyses of 16S rRNA gene amplicons and 16S rRNA metagenomic reads showed the dominance of sulfur-oxidizing Ectothiorhodospiraceae, Thiomicrorhabdus, Sulfurimonas, and Sulfurovum on the wall of two active hydrothermal chimneys. Compared with the inactive hydrothermal sediments of SWIR, the active hydrothermal chimneys lacked sulfur-reducing bacteria. The metabolic potentials of the retrieved 82 metagenome-assembled genomes (MAGs) suggest that sulfur oxidation might be conducted by Thiohalomonadales (classified as Ectothiorhodospiraceae based on 16S rRNA gene amplicons), Sulfurovaceae, Hyphomicrobiaceae, Thiotrichaceae, Thiomicrospiraceae, and Rhodobacteraceae. For CO2 fixation, the Calvin-Benson-Bassham and reductive TCA pathways were employed by these bacteria. In Thiohalomonadales MAGs, we revealed putative phytochrome, carotenoid precursor, and squalene synthesis pathways, indicating a possible capacity of Thiohalomonadales in adaptation to dynamics redox conditions and the utilization of red light from the hot hydrothermal chimneys for photolithotrophic growth. This study, therefore, reveals unique microbiomes and their genomic features in the active hydrothermal chimneys of SWIR, which casts light on ecosystem establishment and development in hydrothermal fields and the deep biosphere.https://www.frontiersin.org/articles/10.3389/fmicb.2022.861795/fulldeep-sea hydrothermal ventsSWIRthiohalomonadalesmetagenomicsred light photosynthesis |
| spellingShingle | Yong Wang Hong-Yu Bi Hua-Guan Chen Hua-Guan Chen Peng-Fei Zheng Ying-Li Zhou Ying-Li Zhou Jiang-Tao Li Metagenomics Reveals Dominant Unusual Sulfur Oxidizers Inhabiting Active Hydrothermal Chimneys From the Southwest Indian Ridge Frontiers in Microbiology deep-sea hydrothermal vents SWIR thiohalomonadales metagenomics red light photosynthesis |
| title | Metagenomics Reveals Dominant Unusual Sulfur Oxidizers Inhabiting Active Hydrothermal Chimneys From the Southwest Indian Ridge |
| title_full | Metagenomics Reveals Dominant Unusual Sulfur Oxidizers Inhabiting Active Hydrothermal Chimneys From the Southwest Indian Ridge |
| title_fullStr | Metagenomics Reveals Dominant Unusual Sulfur Oxidizers Inhabiting Active Hydrothermal Chimneys From the Southwest Indian Ridge |
| title_full_unstemmed | Metagenomics Reveals Dominant Unusual Sulfur Oxidizers Inhabiting Active Hydrothermal Chimneys From the Southwest Indian Ridge |
| title_short | Metagenomics Reveals Dominant Unusual Sulfur Oxidizers Inhabiting Active Hydrothermal Chimneys From the Southwest Indian Ridge |
| title_sort | metagenomics reveals dominant unusual sulfur oxidizers inhabiting active hydrothermal chimneys from the southwest indian ridge |
| topic | deep-sea hydrothermal vents SWIR thiohalomonadales metagenomics red light photosynthesis |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.861795/full |
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