Impedimetric and amperometric genosensors for the highly sensitive quantification of SARS-CoV-2 nucleic acid using an avidin-functionalized multi-walled carbon nanotubes biocapture platform
We report two novel genosensors for the quantification of SARS-CoV-2 nucleic acid using glassy carbon electrodes modified with a biocapture nanoplatform made of multi-walled carbon nanotubes (MWCNTs) non-covalently functionalized with avidin (Av) as a support of the biotinylated-DNA probes. One of t...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Biosensors and Bioelectronics: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590137022001157 |
| _version_ | 1811177412776427520 |
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| author | Michael López Mujica Alejandro Tamborelli Andrés Castellaro Danilo Barcudi María D. Rubianes Marcela C. Rodríguez Héctor A. Saka José L. Bocco Pablo R. Dalmasso Gustavo A. Rivas |
| author_facet | Michael López Mujica Alejandro Tamborelli Andrés Castellaro Danilo Barcudi María D. Rubianes Marcela C. Rodríguez Héctor A. Saka José L. Bocco Pablo R. Dalmasso Gustavo A. Rivas |
| author_sort | Michael López Mujica |
| collection | DOAJ |
| description | We report two novel genosensors for the quantification of SARS-CoV-2 nucleic acid using glassy carbon electrodes modified with a biocapture nanoplatform made of multi-walled carbon nanotubes (MWCNTs) non-covalently functionalized with avidin (Av) as a support of the biotinylated-DNA probes. One of the genosensors was based on impedimetric transduction offering a non-labelled and non-amplified detection of SARS-CoV-2 nucleic acid through the increment of [Fe(CN)6]3-/4- charge transfer resistance. This biosensor presented an excellent analytical performance, with a linear range of 1.0 × 10−18 M - 1.0 × 10−11 M, a sensitivity of (5.8 ± 0.6) x 102 Ω M−1 (r2 = 0.994), detection and quantification limits of 0.33 aM and 1.0 aM, respectively; and reproducibilities of 5.4% for 1.0 × 10−15 M target using the same MWCNTs-Av-bDNAp nanoplatform, and 6.9% for 1.0 × 10−15 M target using 3 different nanoplatforms. The other genosensor was based on a sandwich hybridization scheme and amperometric transduction using the streptavidin(Strep)-biotinylated horseradish peroxidase (bHRP)/hydrogen peroxide/hydroquinone (HQ) system. This genosensor allowed an extremely sensitive quantification of the SARS-CoV-2 nucleic acid, with a linear range of 1.0 × 10−20 M - 1.0 × 10−17 M, detection limit at zM level, and a reproducibility of 11% for genosensors prepared with the same MWCNTs-Av-bDNAp1 nanoplatform. As a proof-of-concept, and considering the extremely high sensitivity, the genosensor was challenged with highly diluted samples obtained from SARS-CoV-2 RNA PCR amplification. |
| first_indexed | 2024-04-11T06:01:18Z |
| format | Article |
| id | doaj.art-d66c8e14b24841e99b61e0e021420e55 |
| institution | Directory Open Access Journal |
| issn | 2590-1370 |
| language | English |
| last_indexed | 2024-04-11T06:01:18Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biosensors and Bioelectronics: X |
| spelling | doaj.art-d66c8e14b24841e99b61e0e021420e552022-12-22T04:41:39ZengElsevierBiosensors and Bioelectronics: X2590-13702022-12-0112100222Impedimetric and amperometric genosensors for the highly sensitive quantification of SARS-CoV-2 nucleic acid using an avidin-functionalized multi-walled carbon nanotubes biocapture platformMichael López Mujica0Alejandro Tamborelli1Andrés Castellaro2Danilo Barcudi3María D. Rubianes4Marcela C. Rodríguez5Héctor A. Saka6José L. Bocco7Pablo R. Dalmasso8Gustavo A. Rivas9INFIQC, CONICET-UNC, Departamento de Fisicoquímica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, 5000, Córdoba, ArgentinaINFIQC, CONICET-UNC, Departamento de Fisicoquímica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, 5000, Córdoba, Argentina; CIQA, CONICET, Departamento de Ingeniería Química, Facultad Regional Córdoba, Universidad Tecnológica Nacional, Maestro López esq. Cruz Roja Argentina, 5016, Córdoba, ArgentinaCIBICI, CONICET-UNC, Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, 5000, Córdoba, ArgentinaCIBICI, CONICET-UNC, Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, 5000, Córdoba, ArgentinaINFIQC, CONICET-UNC, Departamento de Fisicoquímica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, 5000, Córdoba, ArgentinaINFIQC, CONICET-UNC, Departamento de Fisicoquímica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, 5000, Córdoba, ArgentinaCIBICI, CONICET-UNC, Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, 5000, Córdoba, ArgentinaCIBICI, CONICET-UNC, Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, 5000, Córdoba, Argentina; Corresponding author.CIQA, CONICET, Departamento de Ingeniería Química, Facultad Regional Córdoba, Universidad Tecnológica Nacional, Maestro López esq. Cruz Roja Argentina, 5016, Córdoba, Argentina; Corresponding author.INFIQC, CONICET-UNC, Departamento de Fisicoquímica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, 5000, Córdoba, Argentina; Corresponding author.We report two novel genosensors for the quantification of SARS-CoV-2 nucleic acid using glassy carbon electrodes modified with a biocapture nanoplatform made of multi-walled carbon nanotubes (MWCNTs) non-covalently functionalized with avidin (Av) as a support of the biotinylated-DNA probes. One of the genosensors was based on impedimetric transduction offering a non-labelled and non-amplified detection of SARS-CoV-2 nucleic acid through the increment of [Fe(CN)6]3-/4- charge transfer resistance. This biosensor presented an excellent analytical performance, with a linear range of 1.0 × 10−18 M - 1.0 × 10−11 M, a sensitivity of (5.8 ± 0.6) x 102 Ω M−1 (r2 = 0.994), detection and quantification limits of 0.33 aM and 1.0 aM, respectively; and reproducibilities of 5.4% for 1.0 × 10−15 M target using the same MWCNTs-Av-bDNAp nanoplatform, and 6.9% for 1.0 × 10−15 M target using 3 different nanoplatforms. The other genosensor was based on a sandwich hybridization scheme and amperometric transduction using the streptavidin(Strep)-biotinylated horseradish peroxidase (bHRP)/hydrogen peroxide/hydroquinone (HQ) system. This genosensor allowed an extremely sensitive quantification of the SARS-CoV-2 nucleic acid, with a linear range of 1.0 × 10−20 M - 1.0 × 10−17 M, detection limit at zM level, and a reproducibility of 11% for genosensors prepared with the same MWCNTs-Av-bDNAp1 nanoplatform. As a proof-of-concept, and considering the extremely high sensitivity, the genosensor was challenged with highly diluted samples obtained from SARS-CoV-2 RNA PCR amplification.http://www.sciencedirect.com/science/article/pii/S2590137022001157SARS-CoV-2Impedimetric biosensorAmperometric biosensorCarbon nanotubesAvidinNanotechnology |
| spellingShingle | Michael López Mujica Alejandro Tamborelli Andrés Castellaro Danilo Barcudi María D. Rubianes Marcela C. Rodríguez Héctor A. Saka José L. Bocco Pablo R. Dalmasso Gustavo A. Rivas Impedimetric and amperometric genosensors for the highly sensitive quantification of SARS-CoV-2 nucleic acid using an avidin-functionalized multi-walled carbon nanotubes biocapture platform Biosensors and Bioelectronics: X SARS-CoV-2 Impedimetric biosensor Amperometric biosensor Carbon nanotubes Avidin Nanotechnology |
| title | Impedimetric and amperometric genosensors for the highly sensitive quantification of SARS-CoV-2 nucleic acid using an avidin-functionalized multi-walled carbon nanotubes biocapture platform |
| title_full | Impedimetric and amperometric genosensors for the highly sensitive quantification of SARS-CoV-2 nucleic acid using an avidin-functionalized multi-walled carbon nanotubes biocapture platform |
| title_fullStr | Impedimetric and amperometric genosensors for the highly sensitive quantification of SARS-CoV-2 nucleic acid using an avidin-functionalized multi-walled carbon nanotubes biocapture platform |
| title_full_unstemmed | Impedimetric and amperometric genosensors for the highly sensitive quantification of SARS-CoV-2 nucleic acid using an avidin-functionalized multi-walled carbon nanotubes biocapture platform |
| title_short | Impedimetric and amperometric genosensors for the highly sensitive quantification of SARS-CoV-2 nucleic acid using an avidin-functionalized multi-walled carbon nanotubes biocapture platform |
| title_sort | impedimetric and amperometric genosensors for the highly sensitive quantification of sars cov 2 nucleic acid using an avidin functionalized multi walled carbon nanotubes biocapture platform |
| topic | SARS-CoV-2 Impedimetric biosensor Amperometric biosensor Carbon nanotubes Avidin Nanotechnology |
| url | http://www.sciencedirect.com/science/article/pii/S2590137022001157 |
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