Perivascular Interstitial Cells of Cajal in Human ColonSummary
Background & Aims: Interstitial cells of Cajal (ICC) closely associate with nerves and smooth muscles to modulate gut motility. In the ICC microenvironment, although the circulating hormones/factors have been shown to influence ICC activities, the association between ICC and microvessels in the...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2015-01-01
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| Series: | Cellular and Molecular Gastroenterology and Hepatology |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352345X14000071 |
| _version_ | 1811194314223517696 |
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| author | Yuan-An Liu Yuan-Chiang Chung Ming-Yin Shen Shien-Tung Pan Chun-Wei Kuo Shih-Jung Peng Pankaj J. Pasricha Shiue-Cheng Tang |
| author_facet | Yuan-An Liu Yuan-Chiang Chung Ming-Yin Shen Shien-Tung Pan Chun-Wei Kuo Shih-Jung Peng Pankaj J. Pasricha Shiue-Cheng Tang |
| author_sort | Yuan-An Liu |
| collection | DOAJ |
| description | Background & Aims: Interstitial cells of Cajal (ICC) closely associate with nerves and smooth muscles to modulate gut motility. In the ICC microenvironment, although the circulating hormones/factors have been shown to influence ICC activities, the association between ICC and microvessels in the gut wall has not been described. We applied three-dimensional (3D) vascular histology with c-kit staining to identify the perivascular ICC and characterize their morphologic and population features in the human colon wall. Methods: Full-thickness colons were obtained from colectomies performed for colorectal cancer. We targeted the colon wall away from the tumor site. Confocal microscopy with optical clearing (use of immersion solution to reduce scattering in optical imaging) was performed to simultaneously reveal the ICC and vascular networks in space. 3D image rendering and projection were digitally conducted to illustrate the ICCâvessel contact patterns. Results: Perivascular ICC were identified in the submucosal border, myenteric plexus, and circular and longitudinal muscles via high-definition 3D microscopy. Through in-depth image projection, we specified two contact patternsâthe intimate cell body-to-vessel contact (type I, 18% of ICC in circular muscle) and the long-distance process-to-vessel contact (type II, 16%)âto classify perivascular ICC. Particularly, type I perivascular ICC were detected with elevated c-kit staining levels and were routinely found in clusters, making them readily distinguishable from other ICC in the network. Conclusions: We propose a new subclass of ICC that closely associates with microvessels in the human colon. Our finding suggests a functional relationship between these mural ICC and microvessels based on the morphologic proximity. Keywords: 3D Histology, c-kit, ICC, Mural Cells |
| first_indexed | 2024-04-12T00:23:48Z |
| format | Article |
| id | doaj.art-d66cf8fac8b541049834c05e7f3ce28b |
| institution | Directory Open Access Journal |
| issn | 2352-345X |
| language | English |
| last_indexed | 2024-04-12T00:23:48Z |
| publishDate | 2015-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cellular and Molecular Gastroenterology and Hepatology |
| spelling | doaj.art-d66cf8fac8b541049834c05e7f3ce28b2022-12-22T03:55:36ZengElsevierCellular and Molecular Gastroenterology and Hepatology2352-345X2015-01-0111102119Perivascular Interstitial Cells of Cajal in Human ColonSummaryYuan-An Liu0Yuan-Chiang Chung1Ming-Yin Shen2Shien-Tung Pan3Chun-Wei Kuo4Shih-Jung Peng5Pankaj J. Pasricha6Shiue-Cheng Tang7Connectomics Research Center, National Tsing Hua University, Hsinchu, TaiwanDepartment of Surgery, Cheng Ching General Hospital, Chung Kang Branch, Taichung, TaiwanDivision of Colorectal Surgery, National Taiwan University Hospital, Hsinchu Branch, Hsinchu, TaiwanDepartment of Pathology, Miaoli General Hospital, Miaoli, TaiwanDepartment of Pathology, National Taiwan University Hospital, Hsinchu Branch, Hsinchu, TaiwanConnectomics Research Center, National Tsing Hua University, Hsinchu, Taiwan; Department of Medical Science, National Tsing Hua University, Hsinchu, TaiwanDivision of Gastroenterology and Hepatology, Johns Hopkins University School of Medicine, Baltimore, MarylandConnectomics Research Center, National Tsing Hua University, Hsinchu, Taiwan; Department of Medical Science, National Tsing Hua University, Hsinchu, Taiwan; Correspondence Address correspondence to: Shiue-Cheng Tang, PhD, National Tsing Hua University, Department of Medical Science, 101, Section 2, Kuang Fu Road, Hsinchu 30013, Taiwan. fax: (886) 3-571-5934.Background & Aims: Interstitial cells of Cajal (ICC) closely associate with nerves and smooth muscles to modulate gut motility. In the ICC microenvironment, although the circulating hormones/factors have been shown to influence ICC activities, the association between ICC and microvessels in the gut wall has not been described. We applied three-dimensional (3D) vascular histology with c-kit staining to identify the perivascular ICC and characterize their morphologic and population features in the human colon wall. Methods: Full-thickness colons were obtained from colectomies performed for colorectal cancer. We targeted the colon wall away from the tumor site. Confocal microscopy with optical clearing (use of immersion solution to reduce scattering in optical imaging) was performed to simultaneously reveal the ICC and vascular networks in space. 3D image rendering and projection were digitally conducted to illustrate the ICCâvessel contact patterns. Results: Perivascular ICC were identified in the submucosal border, myenteric plexus, and circular and longitudinal muscles via high-definition 3D microscopy. Through in-depth image projection, we specified two contact patternsâthe intimate cell body-to-vessel contact (type I, 18% of ICC in circular muscle) and the long-distance process-to-vessel contact (type II, 16%)âto classify perivascular ICC. Particularly, type I perivascular ICC were detected with elevated c-kit staining levels and were routinely found in clusters, making them readily distinguishable from other ICC in the network. Conclusions: We propose a new subclass of ICC that closely associates with microvessels in the human colon. Our finding suggests a functional relationship between these mural ICC and microvessels based on the morphologic proximity. Keywords: 3D Histology, c-kit, ICC, Mural Cellshttp://www.sciencedirect.com/science/article/pii/S2352345X14000071 |
| spellingShingle | Yuan-An Liu Yuan-Chiang Chung Ming-Yin Shen Shien-Tung Pan Chun-Wei Kuo Shih-Jung Peng Pankaj J. Pasricha Shiue-Cheng Tang Perivascular Interstitial Cells of Cajal in Human ColonSummary Cellular and Molecular Gastroenterology and Hepatology |
| title | Perivascular Interstitial Cells of Cajal in Human ColonSummary |
| title_full | Perivascular Interstitial Cells of Cajal in Human ColonSummary |
| title_fullStr | Perivascular Interstitial Cells of Cajal in Human ColonSummary |
| title_full_unstemmed | Perivascular Interstitial Cells of Cajal in Human ColonSummary |
| title_short | Perivascular Interstitial Cells of Cajal in Human ColonSummary |
| title_sort | perivascular interstitial cells of cajal in human colonsummary |
| url | http://www.sciencedirect.com/science/article/pii/S2352345X14000071 |
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