A simple and rapid extraction of lipids in plasma using spin column with superabsorbent polymer beads for mass spectrometry
Abstract The efficient extraction of lipid species in plasma was developed for quick sample preparation and accurate lipid analysis, using spin column with superabsorbent polymer beads (SAP), as the improvement of previous SAP method reported before. The modified SAP (mSAP) method is composed of sim...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2023-05-01
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| Series: | Journal of Analytical Science and Technology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40543-023-00388-z |
| _version_ | 1797817945417580544 |
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| author | Jung Hoon Choi Geul Bang Jeong Ah Kim Young Hwan Kim |
| author_facet | Jung Hoon Choi Geul Bang Jeong Ah Kim Young Hwan Kim |
| author_sort | Jung Hoon Choi |
| collection | DOAJ |
| description | Abstract The efficient extraction of lipid species in plasma was developed for quick sample preparation and accurate lipid analysis, using spin column with superabsorbent polymer beads (SAP), as the improvement of previous SAP method reported before. The modified SAP (mSAP) method is composed of simple steps, including sample loading, swelling, solvent addition, centrifugation, and drying of the resulting lipid solution. The mSAP method offers several advantages over the conventional and the previous SAP methods for lipid analysis in plasma sample. The mSAP method was approximately 10 times faster than the modified Folch protocol (Matyash method), methyl-tert-butyl ether based extraction method. The mSAP method offered an excellent recovery rate for most all major classes in the lipid standard mixture, outperforming the Matyash method. The limit of detection (LOD) of 1,2-diheptadecanoyl-sn-glycero-3-phophocholine (PC 17:0/17:0) spiking in plasma by the mSAP method is about seven times lower than those of the conventional methods. The relative standard deviation (RSD) values for inter- and intra-day variability of the mSAP method over a 5-day period were significantly lower than those of the previous SAP and Matyash methods. The mSAP method has been shown to be more time-saving, sensitive, reproducible and reliable, as evidence by its faster processing time, lower LOD, lower RSD values and high recovery rate for different lipid classes. Overall, these findings suggest that this method may be a promising approach for the application of total lipid analysis in lipidomic research in future. |
| first_indexed | 2024-03-13T09:02:04Z |
| format | Article |
| id | doaj.art-d670028c4b31418facb60dbe15d721c1 |
| institution | Directory Open Access Journal |
| issn | 2093-3371 |
| language | English |
| last_indexed | 2024-03-13T09:02:04Z |
| publishDate | 2023-05-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Journal of Analytical Science and Technology |
| spelling | doaj.art-d670028c4b31418facb60dbe15d721c12023-05-28T11:19:16ZengSpringerOpenJournal of Analytical Science and Technology2093-33712023-05-0114111010.1186/s40543-023-00388-zA simple and rapid extraction of lipids in plasma using spin column with superabsorbent polymer beads for mass spectrometryJung Hoon Choi0Geul Bang1Jeong Ah Kim2Young Hwan Kim3Bio-Chemical Analysis Team, Korea Basic Science InstituteResearch Center for Bioconvergence Analysis, Korea Basic Science InstituteCenter for Scientific Instrumentation, Korea Basic Science InstituteBio-Chemical Analysis Team, Korea Basic Science InstituteAbstract The efficient extraction of lipid species in plasma was developed for quick sample preparation and accurate lipid analysis, using spin column with superabsorbent polymer beads (SAP), as the improvement of previous SAP method reported before. The modified SAP (mSAP) method is composed of simple steps, including sample loading, swelling, solvent addition, centrifugation, and drying of the resulting lipid solution. The mSAP method offers several advantages over the conventional and the previous SAP methods for lipid analysis in plasma sample. The mSAP method was approximately 10 times faster than the modified Folch protocol (Matyash method), methyl-tert-butyl ether based extraction method. The mSAP method offered an excellent recovery rate for most all major classes in the lipid standard mixture, outperforming the Matyash method. The limit of detection (LOD) of 1,2-diheptadecanoyl-sn-glycero-3-phophocholine (PC 17:0/17:0) spiking in plasma by the mSAP method is about seven times lower than those of the conventional methods. The relative standard deviation (RSD) values for inter- and intra-day variability of the mSAP method over a 5-day period were significantly lower than those of the previous SAP and Matyash methods. The mSAP method has been shown to be more time-saving, sensitive, reproducible and reliable, as evidence by its faster processing time, lower LOD, lower RSD values and high recovery rate for different lipid classes. Overall, these findings suggest that this method may be a promising approach for the application of total lipid analysis in lipidomic research in future.https://doi.org/10.1186/s40543-023-00388-zLipid extractionSuperabsorbent polymersSpin columnLiquid chromatographyMass spectrometry |
| spellingShingle | Jung Hoon Choi Geul Bang Jeong Ah Kim Young Hwan Kim A simple and rapid extraction of lipids in plasma using spin column with superabsorbent polymer beads for mass spectrometry Journal of Analytical Science and Technology Lipid extraction Superabsorbent polymers Spin column Liquid chromatography Mass spectrometry |
| title | A simple and rapid extraction of lipids in plasma using spin column with superabsorbent polymer beads for mass spectrometry |
| title_full | A simple and rapid extraction of lipids in plasma using spin column with superabsorbent polymer beads for mass spectrometry |
| title_fullStr | A simple and rapid extraction of lipids in plasma using spin column with superabsorbent polymer beads for mass spectrometry |
| title_full_unstemmed | A simple and rapid extraction of lipids in plasma using spin column with superabsorbent polymer beads for mass spectrometry |
| title_short | A simple and rapid extraction of lipids in plasma using spin column with superabsorbent polymer beads for mass spectrometry |
| title_sort | simple and rapid extraction of lipids in plasma using spin column with superabsorbent polymer beads for mass spectrometry |
| topic | Lipid extraction Superabsorbent polymers Spin column Liquid chromatography Mass spectrometry |
| url | https://doi.org/10.1186/s40543-023-00388-z |
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