| Summary: | Tilapia is the second most farmed fish species after carp in the world. However, the production has come under threat due to emerging diseases such as tilapia lake virus (TiLV) that causes massive mortalities with high economic losses. It is largely unknown whether different tilapia strains are equally susceptible to TiLV infection. In the present study we compared the susceptibility of gray (<i>Oreochromis niloticus</i> x <i>O. aureus</i>) and red tilapia (<i>Oreochromis</i> spp.) to experimental TiLV infection. Virus was injected intraperitoneally at a concentration of 10<sup>4</sup> TCID<sub>50</sub>/mL. Our findings show that gray tilapia had a lower mortality, 86.44%, but statistically not significantly different (<i>p</i> = 0.068) from red tilapia (100%). The duration of the mortality period from onset to cessation was similar for the two species, starting at 2−3 days post challenge (dpc) with a median at 10−11 dpi and ending on 20−22 dpi. In addition, there was no difference between species in mean viral loads in brain, liver and headkidney from fish collected soon after death. As for host response, expression levels of IL-1β and TNFα were equally high in brain and headkidney samples while levels in liver samples were low for both red and gray tilapia, which coincides with lower viral loads in liver compared to brain and headkidney for both species. We find that red and gray tilapia were equally susceptible to TiLV infection with similar post challenge mortality levels, equal virus concentration in target organs and similar proinflammatory cytokine responses in target and lymphoid organs at time of death. Nonetheless, we advocate that the search for less susceptible tilapia strains should continue with the view to reduce losses from TiLV infection in aquaculture.
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