Amylase Production by Aspergillus niger and Penicillium Species by Solid-State and Submerged Cultivation Using Two Food Industrial Wastes
Amylase enzymes are starch degrading enzymes and have received a great deal of attention due to their perceived technology importance and economic benefit. Amylase enzymes are considered important enzymes used in starch processing industries for the hydrolysis of polysaccharides like starch into sim...
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| Format: | Article |
| Language: | English |
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Technoscience Publications
2021-09-01
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| Series: | Nature Environment and Pollution Technology |
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| Online Access: | https://neptjournal.com/upload-images/(20)B-3768.pdf |
| _version_ | 1818731580877701120 |
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| author | J. Mary Sheela, K. Divya and S. Premina |
| author_facet | J. Mary Sheela, K. Divya and S. Premina |
| author_sort | J. Mary Sheela, K. Divya and S. Premina |
| collection | DOAJ |
| description | Amylase enzymes are starch degrading enzymes and have received a great deal of attention due to their perceived technology importance and economic benefit. Amylase enzymes are considered important enzymes used in starch processing industries for the hydrolysis of polysaccharides like starch into simple sugar constituents. This enzyme is also involved in the commercial production of glucose. Solid-state cultivation and submerged cultivation have tremendous potentials for enzyme amylase production by using different solid substrates like rice bran, wheat bran, coconut oil cake, and groundnut oil cake which are rich in starch. These agro-industrial wastes are considered cheap raw materials for the production of amylase. Wastewater from the industry like brewery can also be used as a liquid substrate for submerged cultivation. It may have the possibility of depurination of wastewater. In the present study, Aspergillus niger and Penicillium species were isolated and their amylase activity was determined by the starch hydrolysis method. Enzyme production was done by using coconut oil cake as a substrate for solid-state fermentation and brewery wastewater as a substrate for submerged fermentation. The enzyme produced by the organisms was extracted and enzyme assay was done by the Dinitrisalicilic method (DNS method). The protein estimation was done by Lowry Folin’s method. The qualitative assay was carried out by performing Gas Chromatography-Mass Spectroscopy (GC-MS). |
| first_indexed | 2024-12-17T23:19:57Z |
| format | Article |
| id | doaj.art-d67225f368494e5aa92faf8c43846c6d |
| institution | Directory Open Access Journal |
| issn | 0972-6268 2395-3454 |
| language | English |
| last_indexed | 2024-12-17T23:19:57Z |
| publishDate | 2021-09-01 |
| publisher | Technoscience Publications |
| record_format | Article |
| series | Nature Environment and Pollution Technology |
| spelling | doaj.art-d67225f368494e5aa92faf8c43846c6d2022-12-21T21:28:55ZengTechnoscience PublicationsNature Environment and Pollution Technology0972-62682395-34542021-09-012031127113510.46488/NEPT.2021.v20i03.020Amylase Production by Aspergillus niger and Penicillium Species by Solid-State and Submerged Cultivation Using Two Food Industrial WastesJ. Mary Sheela, K. Divya and S. PreminaAmylase enzymes are starch degrading enzymes and have received a great deal of attention due to their perceived technology importance and economic benefit. Amylase enzymes are considered important enzymes used in starch processing industries for the hydrolysis of polysaccharides like starch into simple sugar constituents. This enzyme is also involved in the commercial production of glucose. Solid-state cultivation and submerged cultivation have tremendous potentials for enzyme amylase production by using different solid substrates like rice bran, wheat bran, coconut oil cake, and groundnut oil cake which are rich in starch. These agro-industrial wastes are considered cheap raw materials for the production of amylase. Wastewater from the industry like brewery can also be used as a liquid substrate for submerged cultivation. It may have the possibility of depurination of wastewater. In the present study, Aspergillus niger and Penicillium species were isolated and their amylase activity was determined by the starch hydrolysis method. Enzyme production was done by using coconut oil cake as a substrate for solid-state fermentation and brewery wastewater as a substrate for submerged fermentation. The enzyme produced by the organisms was extracted and enzyme assay was done by the Dinitrisalicilic method (DNS method). The protein estimation was done by Lowry Folin’s method. The qualitative assay was carried out by performing Gas Chromatography-Mass Spectroscopy (GC-MS).https://neptjournal.com/upload-images/(20)B-3768.pdfamylase enzyme, coconut oil cake (cw), brewery wastewater (bw), penicillium species, aspergillus niger |
| spellingShingle | J. Mary Sheela, K. Divya and S. Premina Amylase Production by Aspergillus niger and Penicillium Species by Solid-State and Submerged Cultivation Using Two Food Industrial Wastes Nature Environment and Pollution Technology amylase enzyme, coconut oil cake (cw), brewery wastewater (bw), penicillium species, aspergillus niger |
| title | Amylase Production by Aspergillus niger and Penicillium Species by Solid-State and Submerged Cultivation Using Two Food Industrial Wastes |
| title_full | Amylase Production by Aspergillus niger and Penicillium Species by Solid-State and Submerged Cultivation Using Two Food Industrial Wastes |
| title_fullStr | Amylase Production by Aspergillus niger and Penicillium Species by Solid-State and Submerged Cultivation Using Two Food Industrial Wastes |
| title_full_unstemmed | Amylase Production by Aspergillus niger and Penicillium Species by Solid-State and Submerged Cultivation Using Two Food Industrial Wastes |
| title_short | Amylase Production by Aspergillus niger and Penicillium Species by Solid-State and Submerged Cultivation Using Two Food Industrial Wastes |
| title_sort | amylase production by aspergillus niger and penicillium species by solid state and submerged cultivation using two food industrial wastes |
| topic | amylase enzyme, coconut oil cake (cw), brewery wastewater (bw), penicillium species, aspergillus niger |
| url | https://neptjournal.com/upload-images/(20)B-3768.pdf |
| work_keys_str_mv | AT jmarysheelakdivyaandspremina amylaseproductionbyaspergillusnigerandpenicilliumspeciesbysolidstateandsubmergedcultivationusingtwofoodindustrialwastes |