The effects of fresh leaf-to-water ratio and heating time on the antifungal and antioxidant activities of betel leaf (Piper betle L.) extract
More than half of the Indonesian people use herbal medicines to maintain their health, including ones that are served immediately after preparation or also known as jamu gendong. This plant-based medicine is prepared traditionally by boiling using different ratios of leaves to water and heating time...
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| Format: | Article |
| Language: | English |
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Universitas Ahmad Dahlan
2020-03-01
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| Series: | Pharmaciana |
| Subjects: | |
| Online Access: | http://journal.uad.ac.id/index.php/PHARMACIANA/article/view/14257/pdf_144 |
| _version_ | 1818381572770889728 |
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| author | Sartini Sartini Nurdiah Khaerawati Rezky Amalia Kamril Novi Febriani |
| author_facet | Sartini Sartini Nurdiah Khaerawati Rezky Amalia Kamril Novi Febriani |
| author_sort | Sartini Sartini |
| collection | DOAJ |
| description | More than half of the Indonesian people use herbal medicines to maintain their health, including ones that are served immediately after preparation or also known as jamu gendong. This plant-based medicine is prepared traditionally by boiling using different ratios of leaves to water and heating time. The study was designed to determine the heating time and the fresh leaf-to-water ratio that exhibited the highest antioxidant and antifungal activities against Candida albicans. Fresh betel leaves were extracted by boiling and infusion methods, with the ratio of 1:5 and 1:10 and the heating time of 5 and 10 minutes for boiling and 15 minutes for infusion. The excess water in the resulting extract was removed using a freeze dryer, then the total phenolic content was determined by Folin-Ciocalteu spectrophotometry. Meanwhile, the antioxidant and antifungal activities were tested by the DPPH method and microdilution, respectively. The results showed that betel leaf extract with the highest yield and polyphenol content was obtained by infusion with the sample-to-water ratio of 1:10, and this was positively correlated with antioxidant activity against DPPH (IC50= 17.4 ppm) and antifungal effect against Candida albicans (MIC= 0.5%) |
| first_indexed | 2024-12-14T02:36:43Z |
| format | Article |
| id | doaj.art-d67997dcdeba4b7f935e8007c22b54ab |
| institution | Directory Open Access Journal |
| issn | 2088-4559 2477-0256 |
| language | English |
| last_indexed | 2024-12-14T02:36:43Z |
| publishDate | 2020-03-01 |
| publisher | Universitas Ahmad Dahlan |
| record_format | Article |
| series | Pharmaciana |
| spelling | doaj.art-d67997dcdeba4b7f935e8007c22b54ab2022-12-21T23:20:07ZengUniversitas Ahmad DahlanPharmaciana2088-45592477-02562020-03-0110111712410.12928/pharmaciana.v10i1.14257The effects of fresh leaf-to-water ratio and heating time on the antifungal and antioxidant activities of betel leaf (Piper betle L.) extractSartini Sartini0Nurdiah Khaerawati1Rezky Amalia Kamril2Novi Febriani3Department of Pharmacy, Faculty of Pharmacy, Hasanuddin UniversityUndergraduate Program, Faculty of Pharmacy, Hasanuddin UniversityUndergraduate Program, Faculty of Pharmacy, Hasanuddin UniversityUndergraduate Program, Faculty of Pharmacy, Hasanuddin UniversityMore than half of the Indonesian people use herbal medicines to maintain their health, including ones that are served immediately after preparation or also known as jamu gendong. This plant-based medicine is prepared traditionally by boiling using different ratios of leaves to water and heating time. The study was designed to determine the heating time and the fresh leaf-to-water ratio that exhibited the highest antioxidant and antifungal activities against Candida albicans. Fresh betel leaves were extracted by boiling and infusion methods, with the ratio of 1:5 and 1:10 and the heating time of 5 and 10 minutes for boiling and 15 minutes for infusion. The excess water in the resulting extract was removed using a freeze dryer, then the total phenolic content was determined by Folin-Ciocalteu spectrophotometry. Meanwhile, the antioxidant and antifungal activities were tested by the DPPH method and microdilution, respectively. The results showed that betel leaf extract with the highest yield and polyphenol content was obtained by infusion with the sample-to-water ratio of 1:10, and this was positively correlated with antioxidant activity against DPPH (IC50= 17.4 ppm) and antifungal effect against Candida albicans (MIC= 0.5%)http://journal.uad.ac.id/index.php/PHARMACIANA/article/view/14257/pdf_144piper betle l.water extractdpph (22-diphenyl-1-picrylhydrazyl)candida albicans |
| spellingShingle | Sartini Sartini Nurdiah Khaerawati Rezky Amalia Kamril Novi Febriani The effects of fresh leaf-to-water ratio and heating time on the antifungal and antioxidant activities of betel leaf (Piper betle L.) extract Pharmaciana piper betle l. water extract dpph (2 2-diphenyl-1-picrylhydrazyl) candida albicans |
| title | The effects of fresh leaf-to-water ratio and heating time on the antifungal and antioxidant activities of betel leaf (Piper betle L.) extract |
| title_full | The effects of fresh leaf-to-water ratio and heating time on the antifungal and antioxidant activities of betel leaf (Piper betle L.) extract |
| title_fullStr | The effects of fresh leaf-to-water ratio and heating time on the antifungal and antioxidant activities of betel leaf (Piper betle L.) extract |
| title_full_unstemmed | The effects of fresh leaf-to-water ratio and heating time on the antifungal and antioxidant activities of betel leaf (Piper betle L.) extract |
| title_short | The effects of fresh leaf-to-water ratio and heating time on the antifungal and antioxidant activities of betel leaf (Piper betle L.) extract |
| title_sort | effects of fresh leaf to water ratio and heating time on the antifungal and antioxidant activities of betel leaf piper betle l extract |
| topic | piper betle l. water extract dpph (2 2-diphenyl-1-picrylhydrazyl) candida albicans |
| url | http://journal.uad.ac.id/index.php/PHARMACIANA/article/view/14257/pdf_144 |
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