A new crotamine-like from the rattlesnake (Crotalus durissus cumanensis) venom causing damages: Qualitative and Quantitative Cytotoxic Studies on subcellular and neuromuscular structures
A quantitative model to expose the adrenal gland sub-cellular alterations produced by crotamine-like (C-L) from rattlesnake venom during 3, 6 and 24 hours (h), and also qualitative changes on mice neuromuscular structures in vivo were observed and calculated by transmission electron microscopy. A p...
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Universidad del Zulia
2022-05-01
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| Series: | Revista Científica |
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| Online Access: | https://produccioncientificaluz.org/index.php/cientifica/article/view/38134 |
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| author | Estefanie García Hector José Finol† Roschman González Alexis Rodríguez-Acosta |
| author_facet | Estefanie García Hector José Finol† Roschman González Alexis Rodríguez-Acosta |
| author_sort | Estefanie García |
| collection | DOAJ |
| description |
A quantitative model to expose the adrenal gland sub-cellular alterations produced by crotamine-like (C-L) from rattlesnake venom during 3, 6 and 24 hours (h), and also qualitative changes on mice neuromuscular structures in vivo were observed and calculated by transmission electron microscopy. A pure crotamine-like (C-L) isoform was obtained using a cationic exchange chromatography column from the rattlesnake Crotalus durissus cumanensis venom. The C-L SDS-PAGE (15.5 %) under non-reduced conditions exhibited a molecular mass of ~3 kDa single band. The C-L in vivo qualitative experiments induced ultrastructural changes in mouse neuromuscular structures at 3, 6 and 24 h, such as reduction in the number of acetylcholine vesicles, disorganisation of the secondary synaptic clefts, enlargement of the sub-sarcolemma space and alteration of the mitochondria morphology, number and cristae. Regarding neurotoxic actions in vivo, the animals injected with C-L presented spastic paralysis of the hind limbs. The quantitative alterations studied on the capillaries, the nucleus, the mitochondria the lipid inclusions, and the smooth endoplasmic reticulum were observed from 3 to 24 h after C-L injection. As far as it is known from the literature review, there are no quantitative records of similar sub-cellular alterations caused by crotamine.
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| first_indexed | 2024-04-10T06:47:45Z |
| format | Article |
| id | doaj.art-d67b502b93574081a228477fe42672be |
| institution | Directory Open Access Journal |
| issn | 0798-2259 2521-9715 |
| language | English |
| last_indexed | 2024-04-10T06:47:45Z |
| publishDate | 2022-05-01 |
| publisher | Universidad del Zulia |
| record_format | Article |
| series | Revista Científica |
| spelling | doaj.art-d67b502b93574081a228477fe42672be2023-02-28T12:27:28ZengUniversidad del ZuliaRevista Científica0798-22592521-97152022-05-013210.52973/rcfcv-e32119A new crotamine-like from the rattlesnake (Crotalus durissus cumanensis) venom causing damages: Qualitative and Quantitative Cytotoxic Studies on subcellular and neuromuscular structuresEstefanie García0Hector José Finol†1Roschman González2Alexis Rodríguez-Acosta3Universidad Central de Venezuela, Faculty of Sciences, Electron Microscopy Centre. Caracas, Bolivarian Republic of VenezuelaUniversidad Central de Venezuela, Faculty of Sciences, Electron Microscopy Centre. Caracas, Bolivarian Republic of VenezuelaUniversidad Central de Venezuela, Faculty of Sciences, Electron Microscopy Centre. Caracas, Bolivarian Republic of VenezuelaUniversidad Central de Venezuela, Anatomical Institute, Laboratory of Immunochemistry and Ultrastructure. Caracas, Bolivarian Republic of Venezuela A quantitative model to expose the adrenal gland sub-cellular alterations produced by crotamine-like (C-L) from rattlesnake venom during 3, 6 and 24 hours (h), and also qualitative changes on mice neuromuscular structures in vivo were observed and calculated by transmission electron microscopy. A pure crotamine-like (C-L) isoform was obtained using a cationic exchange chromatography column from the rattlesnake Crotalus durissus cumanensis venom. The C-L SDS-PAGE (15.5 %) under non-reduced conditions exhibited a molecular mass of ~3 kDa single band. The C-L in vivo qualitative experiments induced ultrastructural changes in mouse neuromuscular structures at 3, 6 and 24 h, such as reduction in the number of acetylcholine vesicles, disorganisation of the secondary synaptic clefts, enlargement of the sub-sarcolemma space and alteration of the mitochondria morphology, number and cristae. Regarding neurotoxic actions in vivo, the animals injected with C-L presented spastic paralysis of the hind limbs. The quantitative alterations studied on the capillaries, the nucleus, the mitochondria the lipid inclusions, and the smooth endoplasmic reticulum were observed from 3 to 24 h after C-L injection. As far as it is known from the literature review, there are no quantitative records of similar sub-cellular alterations caused by crotamine. https://produccioncientificaluz.org/index.php/cientifica/article/view/38134Crotamine-like peptideCrotalus durissus cumanensismitochondriamotor- endplatemyotoxinrattlesnake |
| spellingShingle | Estefanie García Hector José Finol† Roschman González Alexis Rodríguez-Acosta A new crotamine-like from the rattlesnake (Crotalus durissus cumanensis) venom causing damages: Qualitative and Quantitative Cytotoxic Studies on subcellular and neuromuscular structures Revista Científica Crotamine-like peptide Crotalus durissus cumanensis mitochondria motor- endplate myotoxin rattlesnake |
| title | A new crotamine-like from the rattlesnake (Crotalus durissus cumanensis) venom causing damages: Qualitative and Quantitative Cytotoxic Studies on subcellular and neuromuscular structures |
| title_full | A new crotamine-like from the rattlesnake (Crotalus durissus cumanensis) venom causing damages: Qualitative and Quantitative Cytotoxic Studies on subcellular and neuromuscular structures |
| title_fullStr | A new crotamine-like from the rattlesnake (Crotalus durissus cumanensis) venom causing damages: Qualitative and Quantitative Cytotoxic Studies on subcellular and neuromuscular structures |
| title_full_unstemmed | A new crotamine-like from the rattlesnake (Crotalus durissus cumanensis) venom causing damages: Qualitative and Quantitative Cytotoxic Studies on subcellular and neuromuscular structures |
| title_short | A new crotamine-like from the rattlesnake (Crotalus durissus cumanensis) venom causing damages: Qualitative and Quantitative Cytotoxic Studies on subcellular and neuromuscular structures |
| title_sort | new crotamine like from the rattlesnake crotalus durissus cumanensis venom causing damages qualitative and quantitative cytotoxic studies on subcellular and neuromuscular structures |
| topic | Crotamine-like peptide Crotalus durissus cumanensis mitochondria motor- endplate myotoxin rattlesnake |
| url | https://produccioncientificaluz.org/index.php/cientifica/article/view/38134 |
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