Mesenchymal stromal cells isolated from chicken peripheral blood secrete bioactive factors with antimicrobial and regenerative properties
Mesenchymal stromal cells (MSCs) are adult multipotent progenitor cells that have been isolated from various tissue sources of many species, primarily mammals. Generally, these cells proliferate extensively in culture and have been shown to secrete bioactive factors that contribute to healing proces...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-08-01
|
| Series: | Frontiers in Veterinary Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2022.949836/full |
| _version_ | 1817997138031804416 |
|---|---|
| author | Rebecca M. Harman Katherine A. Churchill Sonia Parmar Gerlinde R. Van de Walle |
| author_facet | Rebecca M. Harman Katherine A. Churchill Sonia Parmar Gerlinde R. Van de Walle |
| author_sort | Rebecca M. Harman |
| collection | DOAJ |
| description | Mesenchymal stromal cells (MSCs) are adult multipotent progenitor cells that have been isolated from various tissue sources of many species, primarily mammals. Generally, these cells proliferate extensively in culture and have been shown to secrete bioactive factors that contribute to healing processes by regulating inflammation, modulating immune responses, inhibiting bacterial growth, and promoting tissue regeneration. The present study reports on the isolation and characterization of MSCs from the peripheral blood (PB) of chickens. Chicken PBMSCs were characterized based on their trilineage differentiation potential and gene and protein expression of MSC-specific cell surface markers. To determine functionality, conditioned medium (CM), which contains all bioactive factors secreted by MSCs, was collected from chicken PBMSCs, and used in in vitro antimicrobial, migration, and angiogenesis assays. Chicken PBMSC CM was found to (i) inhibit the growth of planktonic Staphylococcus aureus (S. aureus), and even more significantly the methicillin-resistant S. aureus (MRSA), (ii) decrease adhesion and promote migration of fibroblasts, and (iii) support endothelial cell tube formation. Collectively, these data indicate that chicken PBMSCs secrete bioactive factors with antimicrobial and regenerative properties, and as such, provide a novel source of cell-based therapies for the poultry industry. |
| first_indexed | 2024-04-14T02:33:47Z |
| format | Article |
| id | doaj.art-d67bd67c9b3e4f438bed7bace6a5ca38 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-04-14T02:33:47Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-d67bd67c9b3e4f438bed7bace6a5ca382022-12-22T02:17:36ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692022-08-01910.3389/fvets.2022.949836949836Mesenchymal stromal cells isolated from chicken peripheral blood secrete bioactive factors with antimicrobial and regenerative propertiesRebecca M. HarmanKatherine A. ChurchillSonia ParmarGerlinde R. Van de WalleMesenchymal stromal cells (MSCs) are adult multipotent progenitor cells that have been isolated from various tissue sources of many species, primarily mammals. Generally, these cells proliferate extensively in culture and have been shown to secrete bioactive factors that contribute to healing processes by regulating inflammation, modulating immune responses, inhibiting bacterial growth, and promoting tissue regeneration. The present study reports on the isolation and characterization of MSCs from the peripheral blood (PB) of chickens. Chicken PBMSCs were characterized based on their trilineage differentiation potential and gene and protein expression of MSC-specific cell surface markers. To determine functionality, conditioned medium (CM), which contains all bioactive factors secreted by MSCs, was collected from chicken PBMSCs, and used in in vitro antimicrobial, migration, and angiogenesis assays. Chicken PBMSC CM was found to (i) inhibit the growth of planktonic Staphylococcus aureus (S. aureus), and even more significantly the methicillin-resistant S. aureus (MRSA), (ii) decrease adhesion and promote migration of fibroblasts, and (iii) support endothelial cell tube formation. Collectively, these data indicate that chicken PBMSCs secrete bioactive factors with antimicrobial and regenerative properties, and as such, provide a novel source of cell-based therapies for the poultry industry.https://www.frontiersin.org/articles/10.3389/fvets.2022.949836/fullchickenmesenchymal stromal cellsperipheral bloodsecreted factorsregenerativeantimicrobial |
| spellingShingle | Rebecca M. Harman Katherine A. Churchill Sonia Parmar Gerlinde R. Van de Walle Mesenchymal stromal cells isolated from chicken peripheral blood secrete bioactive factors with antimicrobial and regenerative properties Frontiers in Veterinary Science chicken mesenchymal stromal cells peripheral blood secreted factors regenerative antimicrobial |
| title | Mesenchymal stromal cells isolated from chicken peripheral blood secrete bioactive factors with antimicrobial and regenerative properties |
| title_full | Mesenchymal stromal cells isolated from chicken peripheral blood secrete bioactive factors with antimicrobial and regenerative properties |
| title_fullStr | Mesenchymal stromal cells isolated from chicken peripheral blood secrete bioactive factors with antimicrobial and regenerative properties |
| title_full_unstemmed | Mesenchymal stromal cells isolated from chicken peripheral blood secrete bioactive factors with antimicrobial and regenerative properties |
| title_short | Mesenchymal stromal cells isolated from chicken peripheral blood secrete bioactive factors with antimicrobial and regenerative properties |
| title_sort | mesenchymal stromal cells isolated from chicken peripheral blood secrete bioactive factors with antimicrobial and regenerative properties |
| topic | chicken mesenchymal stromal cells peripheral blood secreted factors regenerative antimicrobial |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2022.949836/full |
| work_keys_str_mv | AT rebeccamharman mesenchymalstromalcellsisolatedfromchickenperipheralbloodsecretebioactivefactorswithantimicrobialandregenerativeproperties AT katherineachurchill mesenchymalstromalcellsisolatedfromchickenperipheralbloodsecretebioactivefactorswithantimicrobialandregenerativeproperties AT soniaparmar mesenchymalstromalcellsisolatedfromchickenperipheralbloodsecretebioactivefactorswithantimicrobialandregenerativeproperties AT gerlindervandewalle mesenchymalstromalcellsisolatedfromchickenperipheralbloodsecretebioactivefactorswithantimicrobialandregenerativeproperties |