In silico Analysis of Immunologic Regions of Surface Antigens (Sags) of Toxoplasma gondii
<h2>Background: Surface antigens (SAGs) of Toxoplasma gondii are known candidates for diagnostic tests and vaccines. The present study argues about the main necessary properties for determination and prediction of T-cell agretopes and B-cell epitopes of surface antigens of Toxoplasma gondii.&l...
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| Format: | Article |
| Language: | English |
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Shahid Beheshti University of Medical Sciences
2017-06-01
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| Series: | Novelty in Biomedicine |
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| Online Access: | http://journals.sbmu.ac.ir/nbm/article/view/14167 |
| _version_ | 1819069121705279488 |
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| author | Abbas Alibakhshi Mojgan Bandehpour Tina Nafarieh Shivasadat Gheflat Bahram Kazemi |
| author_facet | Abbas Alibakhshi Mojgan Bandehpour Tina Nafarieh Shivasadat Gheflat Bahram Kazemi |
| author_sort | Abbas Alibakhshi |
| collection | DOAJ |
| description | <h2>Background: Surface antigens (SAGs) of Toxoplasma gondii are known candidates for diagnostic tests and vaccines. The present study argues about the main necessary properties for determination and prediction of T-cell agretopes and B-cell epitopes of surface antigens of Toxoplasma gondii.</h2><p><strong>M</strong><strong>aterials and Methods: </strong>Primary, secondary and tertiary structures of the proteins were analyzed by different methods. The three-dimensional structures were determined by use of <em>ab initio</em> method for prediction of discontinues epitopes. The agretopes and epitopes were predicted via several various web servers with different methods employed.</p><p><strong>R</strong><strong>es</strong><strong>ults: </strong>The results of <em>in silico</em> analyses showed that the regions 129-GAPAGRNNDGSSAPT-143 for protein p22, 234-SENPWQGNASSD-245 for protein p30 and 348-PGTEGESQAGT-358 for protein p43, have the highest immunogenic potential.</p><p><strong>C</strong><strong>onclusion: </strong>We reached to three antigenic epitopes for cloning and protein expression. In following the purified polypeptide will be applied for diagnosis of Toxoplasma gondii.</p> |
| first_indexed | 2024-12-21T16:45:01Z |
| format | Article |
| id | doaj.art-d67daa15f0bd47a6a361ce7de5076594 |
| institution | Directory Open Access Journal |
| issn | 2345-3346 2345-3907 |
| language | English |
| last_indexed | 2024-12-21T16:45:01Z |
| publishDate | 2017-06-01 |
| publisher | Shahid Beheshti University of Medical Sciences |
| record_format | Article |
| series | Novelty in Biomedicine |
| spelling | doaj.art-d67daa15f0bd47a6a361ce7de50765942022-12-21T18:57:00ZengShahid Beheshti University of Medical SciencesNovelty in Biomedicine2345-33462345-39072017-06-015310911810.22037/nbm.v5i3.141678933In silico Analysis of Immunologic Regions of Surface Antigens (Sags) of Toxoplasma gondiiAbbas Alibakhshi0Mojgan Bandehpour1Tina Nafarieh2Shivasadat Gheflat3Bahram Kazemi4Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Biotechnology, School of Advanced Technologies in Medicine, and Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Biotechnology, School of Advanced Technologies in Medicine, and Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran<h2>Background: Surface antigens (SAGs) of Toxoplasma gondii are known candidates for diagnostic tests and vaccines. The present study argues about the main necessary properties for determination and prediction of T-cell agretopes and B-cell epitopes of surface antigens of Toxoplasma gondii.</h2><p><strong>M</strong><strong>aterials and Methods: </strong>Primary, secondary and tertiary structures of the proteins were analyzed by different methods. The three-dimensional structures were determined by use of <em>ab initio</em> method for prediction of discontinues epitopes. The agretopes and epitopes were predicted via several various web servers with different methods employed.</p><p><strong>R</strong><strong>es</strong><strong>ults: </strong>The results of <em>in silico</em> analyses showed that the regions 129-GAPAGRNNDGSSAPT-143 for protein p22, 234-SENPWQGNASSD-245 for protein p30 and 348-PGTEGESQAGT-358 for protein p43, have the highest immunogenic potential.</p><p><strong>C</strong><strong>onclusion: </strong>We reached to three antigenic epitopes for cloning and protein expression. In following the purified polypeptide will be applied for diagnosis of Toxoplasma gondii.</p>http://journals.sbmu.ac.ir/nbm/article/view/14167Epitope, Agretope, SAG, Toxoplasma gondii, In silico |
| spellingShingle | Abbas Alibakhshi Mojgan Bandehpour Tina Nafarieh Shivasadat Gheflat Bahram Kazemi In silico Analysis of Immunologic Regions of Surface Antigens (Sags) of Toxoplasma gondii Novelty in Biomedicine Epitope, Agretope, SAG, Toxoplasma gondii, In silico |
| title | In silico Analysis of Immunologic Regions of Surface Antigens (Sags) of Toxoplasma gondii |
| title_full | In silico Analysis of Immunologic Regions of Surface Antigens (Sags) of Toxoplasma gondii |
| title_fullStr | In silico Analysis of Immunologic Regions of Surface Antigens (Sags) of Toxoplasma gondii |
| title_full_unstemmed | In silico Analysis of Immunologic Regions of Surface Antigens (Sags) of Toxoplasma gondii |
| title_short | In silico Analysis of Immunologic Regions of Surface Antigens (Sags) of Toxoplasma gondii |
| title_sort | in silico analysis of immunologic regions of surface antigens sags of toxoplasma gondii |
| topic | Epitope, Agretope, SAG, Toxoplasma gondii, In silico |
| url | http://journals.sbmu.ac.ir/nbm/article/view/14167 |
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