Biofilm removal from Difficult-to-Reach places via secondary cavitation within a constrained geometry mimicking a Periodontal/Peri-Implant pocket
Biofilm removal from the apical region of the periodontal or peri-implant pocket, which is very difficult to achieve with mechanical instruments, is a major unresolved issue in dentistry. Here, we propose the use of photoacoustically induced streaming and secondary cavitation to achieve superior cle...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Ultrasonics Sonochemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1350417724000804 |
| _version_ | 1797245012692434944 |
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| author | Marko Volk Katja Molan Dominik Šavli Saša Terlep Špela Levičnik-Höfferle Boris Gašpirc Matjaž Lukač Matija Jezeršek David Stopar |
| author_facet | Marko Volk Katja Molan Dominik Šavli Saša Terlep Špela Levičnik-Höfferle Boris Gašpirc Matjaž Lukač Matija Jezeršek David Stopar |
| author_sort | Marko Volk |
| collection | DOAJ |
| description | Biofilm removal from the apical region of the periodontal or peri-implant pocket, which is very difficult to achieve with mechanical instruments, is a major unresolved issue in dentistry. Here, we propose the use of photoacoustically induced streaming and secondary cavitation to achieve superior cleaning efficacy in the apical region of the periodontal and peri-implant pocket. We have used a prefabricated narrow wedge system that mimics the consistency of periodontal and peri-implant pockets of both healthy and severely inflamed tissue. We studied the effect of single-pulse modality Er:YAG on Pseudomonas aeruginosa biofilm removal. We used different laser energies, fiber-tip positions, and laser treatment durations. The cleaning process was monitored in real-time with a high-speed camera after each individual laser pulse application. The obtained results suggest that biofilm cleaning efficacy in a difficult-to-reach place in healthy model tissue is directly related to the onset of secondary cavitation bubble formation, which correlates with a significant improvement of biofilm removal from the apical region of the periodontal or peri-implant pocket. In comparison to the healthy tissue model, the laser energy in inflamed tissue model had to be increased to obtain comparable biofilm cleaning efficacy. The advantage of photoacoustic cavitation compared to other methods is that laser-induced cavitation can trigger secondary cavitation at large distances from the point of laser application, which in principle allows biofilm removal at distant locations not reachable with a laser fiber tip or other mechanical instruments. |
| first_indexed | 2024-04-24T19:20:08Z |
| format | Article |
| id | doaj.art-d67e6f551c0c4684b3b1f0c0eea96fcc |
| institution | Directory Open Access Journal |
| issn | 1350-4177 |
| language | English |
| last_indexed | 2024-04-24T19:20:08Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ultrasonics Sonochemistry |
| spelling | doaj.art-d67e6f551c0c4684b3b1f0c0eea96fcc2024-03-26T04:26:37ZengElsevierUltrasonics Sonochemistry1350-41772024-03-01104106832Biofilm removal from Difficult-to-Reach places via secondary cavitation within a constrained geometry mimicking a Periodontal/Peri-Implant pocketMarko Volk0Katja Molan1Dominik Šavli2Saša Terlep3Špela Levičnik-Höfferle4Boris Gašpirc5Matjaž Lukač6Matija Jezeršek7David Stopar8University of Ljubljana, Biotechnical Faculty, Department of Microbiology, Večna pot 111, Ljubljana 1000, SloveniaUniversity of Ljubljana, Biotechnical Faculty, Department of Microbiology, Večna pot 111, Ljubljana 1000, SloveniaUniversity of Ljubljana, Faculty of Mechanical Engineering, Aškerčeva cesta 6, Ljubljana 1000, SloveniaFotona d.o.o., Stegne 7, Ljubljana 1000, SloveniaFotona d.o.o., Stegne 7, Ljubljana 1000, SloveniaUniversity of Ljubljana, Medical Faculty, Department of Oral Medicine and Periodontology, Vrazov trg 2, 1000 Ljubljana, SloveniaFotona d.o.o., Stegne 7, Ljubljana 1000, Slovenia; Institut Jozef Stefan, Jamova 39, Ljubljana 1000, Slovenia; University of Ljubljana, Faculty of Mathematics and Physics, Jadranska 19, Ljubljana 1000, SloveniaUniversity of Ljubljana, Faculty of Mechanical Engineering, Aškerčeva cesta 6, Ljubljana 1000, SloveniaUniversity of Ljubljana, Biotechnical Faculty, Department of Microbiology, Večna pot 111, Ljubljana 1000, Slovenia; Corresponding author at: Chair of Microbiology, Department of Microbiology, Biotechnical Faculty, University of Ljubljana, Večna pot 111, Ljubljana 1000, Slovenia.Biofilm removal from the apical region of the periodontal or peri-implant pocket, which is very difficult to achieve with mechanical instruments, is a major unresolved issue in dentistry. Here, we propose the use of photoacoustically induced streaming and secondary cavitation to achieve superior cleaning efficacy in the apical region of the periodontal and peri-implant pocket. We have used a prefabricated narrow wedge system that mimics the consistency of periodontal and peri-implant pockets of both healthy and severely inflamed tissue. We studied the effect of single-pulse modality Er:YAG on Pseudomonas aeruginosa biofilm removal. We used different laser energies, fiber-tip positions, and laser treatment durations. The cleaning process was monitored in real-time with a high-speed camera after each individual laser pulse application. The obtained results suggest that biofilm cleaning efficacy in a difficult-to-reach place in healthy model tissue is directly related to the onset of secondary cavitation bubble formation, which correlates with a significant improvement of biofilm removal from the apical region of the periodontal or peri-implant pocket. In comparison to the healthy tissue model, the laser energy in inflamed tissue model had to be increased to obtain comparable biofilm cleaning efficacy. The advantage of photoacoustic cavitation compared to other methods is that laser-induced cavitation can trigger secondary cavitation at large distances from the point of laser application, which in principle allows biofilm removal at distant locations not reachable with a laser fiber tip or other mechanical instruments.http://www.sciencedirect.com/science/article/pii/S1350417724000804Biofilm removalPeriodontal pocketPeri-implant pocketCavitationPhotoacoustic cleaningEr:YAG USP |
| spellingShingle | Marko Volk Katja Molan Dominik Šavli Saša Terlep Špela Levičnik-Höfferle Boris Gašpirc Matjaž Lukač Matija Jezeršek David Stopar Biofilm removal from Difficult-to-Reach places via secondary cavitation within a constrained geometry mimicking a Periodontal/Peri-Implant pocket Ultrasonics Sonochemistry Biofilm removal Periodontal pocket Peri-implant pocket Cavitation Photoacoustic cleaning Er:YAG USP |
| title | Biofilm removal from Difficult-to-Reach places via secondary cavitation within a constrained geometry mimicking a Periodontal/Peri-Implant pocket |
| title_full | Biofilm removal from Difficult-to-Reach places via secondary cavitation within a constrained geometry mimicking a Periodontal/Peri-Implant pocket |
| title_fullStr | Biofilm removal from Difficult-to-Reach places via secondary cavitation within a constrained geometry mimicking a Periodontal/Peri-Implant pocket |
| title_full_unstemmed | Biofilm removal from Difficult-to-Reach places via secondary cavitation within a constrained geometry mimicking a Periodontal/Peri-Implant pocket |
| title_short | Biofilm removal from Difficult-to-Reach places via secondary cavitation within a constrained geometry mimicking a Periodontal/Peri-Implant pocket |
| title_sort | biofilm removal from difficult to reach places via secondary cavitation within a constrained geometry mimicking a periodontal peri implant pocket |
| topic | Biofilm removal Periodontal pocket Peri-implant pocket Cavitation Photoacoustic cleaning Er:YAG USP |
| url | http://www.sciencedirect.com/science/article/pii/S1350417724000804 |
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