Efficient Method for Generating Point Mutations in the Vaccinia Virus Genome Using CRISPR/Cas9

Efficient Method for Generating Point Mutations in the Vaccinia Virus Genome Using CRISPR/Cas9

The vaccinia virus (VACV) was previously used as a vaccine for smallpox eradication. Nowadays, recombinant VACVs are developed as vaccine platforms for infectious disease prevention and cancer treatment. The conventional method for genome editing of the VACV is based on homologous recombination, whi...

Full description

Bibliographic Details
Main Authors: Laetitia Boutin, Estelle Mosca, Frédéric Iseni
Format: Article
Language:English
Published: MDPI AG 2022-07-01
Series:Viruses
Subjects:
vaccinia virus
CRISPR-Cas9
homologous recombination
PAM sequence
Online Access:https://www.mdpi.com/1999-4915/14/7/1559

Similar Items

Similar Items