Re-decontamination of liquid mycobacterial cultures: Additional Mycobacterium tuberculosis yield in the era of Xpert MTB/RIF Ultra in Cape Town, South Africa
A retrospective review of liquid mycobacterial cultures was performed at a laboratory in South Africa from 01 January 2018 to 31 December 2018 to assess the increased yield in detecting Mycobacterium tuberculosis complex following sample re-decontamination. Only 9 of 99 (9%) re-decontaminated sample...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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AOSIS
2021-12-01
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| Series: | African Journal of Laboratory Medicine |
| Subjects: | |
| Online Access: | https://ajlmonline.org/index.php/ajlm/article/view/1529 |
| _version_ | 1811301282521022464 |
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| author | Dawood da Costa Pieter Nel |
| author_facet | Dawood da Costa Pieter Nel |
| author_sort | Dawood da Costa |
| collection | DOAJ |
| description | A retrospective review of liquid mycobacterial cultures was performed at a laboratory in South Africa from 01 January 2018 to 31 December 2018 to assess the increased yield in detecting Mycobacterium tuberculosis complex following sample re-decontamination. Only 9 of 99 (9%) re-decontaminated samples were culture positive for M. tuberculosis complex. Xpert MTB/RIF Ultra, concurrently performed on 7 of the 9 samples, detected M. tuberculosis complex in all but 1 sample. Re-decontamination of non-sterile samples did not increase the M. tuberculosis complex yield enough to offset the financial costs and additional labour in a laboratory that utilises the Xpert MTB/RIF Ultra system as a first-line diagnostic modality. |
| first_indexed | 2024-04-13T07:05:58Z |
| format | Article |
| id | doaj.art-d67f711866c140b7a25032168605fe40 |
| institution | Directory Open Access Journal |
| issn | 2225-2002 2225-2010 |
| language | English |
| last_indexed | 2024-04-13T07:05:58Z |
| publishDate | 2021-12-01 |
| publisher | AOSIS |
| record_format | Article |
| series | African Journal of Laboratory Medicine |
| spelling | doaj.art-d67f711866c140b7a25032168605fe402022-12-22T02:56:59ZengAOSISAfrican Journal of Laboratory Medicine2225-20022225-20102021-12-01101e1e310.4102/ajlm.v10i1.1529345Re-decontamination of liquid mycobacterial cultures: Additional Mycobacterium tuberculosis yield in the era of Xpert MTB/RIF Ultra in Cape Town, South AfricaDawood da Costa0Pieter Nel1Division of Medical Microbiology and Immunology, Department of Pathology, Faculty of Medical and Health Sciences, Stellenbosch University, Cape Town, South Africa; and, National Health Laboratory Service, Tygerberg Hospital, Cape TownDivision of Medical Microbiology and Immunology, Department of Pathology, Faculty of Medical and Health Sciences, Stellenbosch University, Cape Town, South Africa; and, National Health Laboratory Service, Tygerberg Hospital, Cape TownA retrospective review of liquid mycobacterial cultures was performed at a laboratory in South Africa from 01 January 2018 to 31 December 2018 to assess the increased yield in detecting Mycobacterium tuberculosis complex following sample re-decontamination. Only 9 of 99 (9%) re-decontaminated samples were culture positive for M. tuberculosis complex. Xpert MTB/RIF Ultra, concurrently performed on 7 of the 9 samples, detected M. tuberculosis complex in all but 1 sample. Re-decontamination of non-sterile samples did not increase the M. tuberculosis complex yield enough to offset the financial costs and additional labour in a laboratory that utilises the Xpert MTB/RIF Ultra system as a first-line diagnostic modality.https://ajlmonline.org/index.php/ajlm/article/view/1529tuberculosis culture, liquid mycobacterial growth indicator tube culture, decontamination, re-decontamination, xpert mtb/rif ultra |
| spellingShingle | Dawood da Costa Pieter Nel Re-decontamination of liquid mycobacterial cultures: Additional Mycobacterium tuberculosis yield in the era of Xpert MTB/RIF Ultra in Cape Town, South Africa African Journal of Laboratory Medicine tuberculosis culture, liquid mycobacterial growth indicator tube culture, decontamination, re-decontamination, xpert mtb/rif ultra |
| title | Re-decontamination of liquid mycobacterial cultures: Additional Mycobacterium tuberculosis yield in the era of Xpert MTB/RIF Ultra in Cape Town, South Africa |
| title_full | Re-decontamination of liquid mycobacterial cultures: Additional Mycobacterium tuberculosis yield in the era of Xpert MTB/RIF Ultra in Cape Town, South Africa |
| title_fullStr | Re-decontamination of liquid mycobacterial cultures: Additional Mycobacterium tuberculosis yield in the era of Xpert MTB/RIF Ultra in Cape Town, South Africa |
| title_full_unstemmed | Re-decontamination of liquid mycobacterial cultures: Additional Mycobacterium tuberculosis yield in the era of Xpert MTB/RIF Ultra in Cape Town, South Africa |
| title_short | Re-decontamination of liquid mycobacterial cultures: Additional Mycobacterium tuberculosis yield in the era of Xpert MTB/RIF Ultra in Cape Town, South Africa |
| title_sort | re decontamination of liquid mycobacterial cultures additional mycobacterium tuberculosis yield in the era of xpert mtb rif ultra in cape town south africa |
| topic | tuberculosis culture, liquid mycobacterial growth indicator tube culture, decontamination, re-decontamination, xpert mtb/rif ultra |
| url | https://ajlmonline.org/index.php/ajlm/article/view/1529 |
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