Mean reticulocyte volume enhances the utility of red cell mean sphered cell volume in differentiating peripheral blood spherocytes of hereditary spherocytosis from other causes
Context: Mean sphered cell volume (MSCV) and mean reticulocyte volume (MRV) are additional reticulocyte parameters generated while processing the blood samples on Beckman coulter LH 755 in the reticulocyte mode using the volume, conductivity and scatter technology. It has been observed that the diff...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2015-01-01
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| Series: | Indian Journal of Pathology and Microbiology |
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| Online Access: | http://www.ijpmonline.org/article.asp?issn=0377-4929;year=2015;volume=58;issue=3;spage=307;epage=309;aulast=Nair |
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| author | Sukesh C Nair Neeraj Arora Sachin Jain David Inbakumar Joy Mammen Usha Sitaram |
| author_facet | Sukesh C Nair Neeraj Arora Sachin Jain David Inbakumar Joy Mammen Usha Sitaram |
| author_sort | Sukesh C Nair |
| collection | DOAJ |
| description | Context: Mean sphered cell volume (MSCV) and mean reticulocyte volume (MRV) are additional reticulocyte parameters generated while processing the blood samples on Beckman coulter LH 755 in the reticulocyte mode using the volume, conductivity and scatter technology. It has been observed that the difference between mean corpuscular volume (MCV) and MSCV is higher in the cases of hereditary spherocytosis (HS) and this difference is increasingly being utilized as a screening tool for spherocytes. In addition now there have been new observations that reticulocyte volume in cases of HS is less as compared to normal reticulocyte. Aims: Our aim was to test the usefulness of reticulocyte parameters like MSCV and MRV in distinguishing cases of HS and autoimmune hemolytic anemia (AIHA). Materials and Methods: This is a retrospective and partly prospective study where peripheral blood ethylenediaminetetraacetic acid samples from cases of HS (n = 57) and AIHA (n = 29) were processed on LH 755 in both the differential and the reticulocyte mode. The data generated were analyzed and compared with data from normal healthy donors (n = 46). Results: Using an algorithm of MCV - MSCV >10 and MRV - MSCV <25, a sensitivity of 84.2% and specificity of 94.7% was observed in cases of HS. Conclusions: With the reticulocyte analysis, we may now have a simple and cheap additional tool for screening of HS. |
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| format | Article |
| id | doaj.art-d6806460c7b845c1bd918a61d56f9b63 |
| institution | Directory Open Access Journal |
| issn | 0377-4929 |
| language | English |
| last_indexed | 2024-04-13T07:14:55Z |
| publishDate | 2015-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Indian Journal of Pathology and Microbiology |
| spelling | doaj.art-d6806460c7b845c1bd918a61d56f9b632022-12-22T02:56:47ZengWolters Kluwer Medknow PublicationsIndian Journal of Pathology and Microbiology0377-49292015-01-0158330730910.4103/0377-4929.162836Mean reticulocyte volume enhances the utility of red cell mean sphered cell volume in differentiating peripheral blood spherocytes of hereditary spherocytosis from other causesSukesh C NairNeeraj AroraSachin JainDavid InbakumarJoy MammenUsha SitaramContext: Mean sphered cell volume (MSCV) and mean reticulocyte volume (MRV) are additional reticulocyte parameters generated while processing the blood samples on Beckman coulter LH 755 in the reticulocyte mode using the volume, conductivity and scatter technology. It has been observed that the difference between mean corpuscular volume (MCV) and MSCV is higher in the cases of hereditary spherocytosis (HS) and this difference is increasingly being utilized as a screening tool for spherocytes. In addition now there have been new observations that reticulocyte volume in cases of HS is less as compared to normal reticulocyte. Aims: Our aim was to test the usefulness of reticulocyte parameters like MSCV and MRV in distinguishing cases of HS and autoimmune hemolytic anemia (AIHA). Materials and Methods: This is a retrospective and partly prospective study where peripheral blood ethylenediaminetetraacetic acid samples from cases of HS (n = 57) and AIHA (n = 29) were processed on LH 755 in both the differential and the reticulocyte mode. The data generated were analyzed and compared with data from normal healthy donors (n = 46). Results: Using an algorithm of MCV - MSCV >10 and MRV - MSCV <25, a sensitivity of 84.2% and specificity of 94.7% was observed in cases of HS. Conclusions: With the reticulocyte analysis, we may now have a simple and cheap additional tool for screening of HS.http://www.ijpmonline.org/article.asp?issn=0377-4929;year=2015;volume=58;issue=3;spage=307;epage=309;aulast=NairAutoimmune hemolytic anemiahereditary spherocytosismean corpuscular volumemean reticulocyte volumemean sphered cell volume |
| spellingShingle | Sukesh C Nair Neeraj Arora Sachin Jain David Inbakumar Joy Mammen Usha Sitaram Mean reticulocyte volume enhances the utility of red cell mean sphered cell volume in differentiating peripheral blood spherocytes of hereditary spherocytosis from other causes Indian Journal of Pathology and Microbiology Autoimmune hemolytic anemia hereditary spherocytosis mean corpuscular volume mean reticulocyte volume mean sphered cell volume |
| title | Mean reticulocyte volume enhances the utility of red cell mean sphered cell volume in differentiating peripheral blood spherocytes of hereditary spherocytosis from other causes |
| title_full | Mean reticulocyte volume enhances the utility of red cell mean sphered cell volume in differentiating peripheral blood spherocytes of hereditary spherocytosis from other causes |
| title_fullStr | Mean reticulocyte volume enhances the utility of red cell mean sphered cell volume in differentiating peripheral blood spherocytes of hereditary spherocytosis from other causes |
| title_full_unstemmed | Mean reticulocyte volume enhances the utility of red cell mean sphered cell volume in differentiating peripheral blood spherocytes of hereditary spherocytosis from other causes |
| title_short | Mean reticulocyte volume enhances the utility of red cell mean sphered cell volume in differentiating peripheral blood spherocytes of hereditary spherocytosis from other causes |
| title_sort | mean reticulocyte volume enhances the utility of red cell mean sphered cell volume in differentiating peripheral blood spherocytes of hereditary spherocytosis from other causes |
| topic | Autoimmune hemolytic anemia hereditary spherocytosis mean corpuscular volume mean reticulocyte volume mean sphered cell volume |
| url | http://www.ijpmonline.org/article.asp?issn=0377-4929;year=2015;volume=58;issue=3;spage=307;epage=309;aulast=Nair |
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