PopP1, a New Member of the YopJ/AvrRxv Family of Type III Effector Proteins, Acts as a Host-Specificity Factor and Modulates Aggressiveness of Ralstonia solanacearum
A functional analysis of an 11-kb-long region of the genome of the plant-pathogenic bacterium Ralstonia solanacearum, previously identified as an alternative codon usage region (ACUR), reveals that it was probably acquired through horizontal gene transfer. This ACUR encodes an insertion sequence and...
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2002-10-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2002.15.10.1058 |
| _version_ | 1828259828167868416 |
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| author | Muriel Lavie Edwin Shillington Cédric Eguiluz Nigel Grimsley Christian Boucher |
| author_facet | Muriel Lavie Edwin Shillington Cédric Eguiluz Nigel Grimsley Christian Boucher |
| author_sort | Muriel Lavie |
| collection | DOAJ |
| description | A functional analysis of an 11-kb-long region of the genome of the plant-pathogenic bacterium Ralstonia solanacearum, previously identified as an alternative codon usage region (ACUR), reveals that it was probably acquired through horizontal gene transfer. This ACUR encodes an insertion sequence and eight potential proteins, one of which is partially homologous with a host-specificity factor from a plant-pathogenic Erwinia sp., and another, PopP1, which is homologous to members of the YopJ/AvrRxv family of type III-secreted bacterial effectors controlling interaction between bacteria and their hosts. The analysis of mutants affecting all except one of the genes identified in the ACUR showed that only the popP1-deficient strain had an altered phenotype in plant infection tests. This mutant strain became pathogenic to a Petunia line that is resistant to the wild-type strain. Therefore, popP1 behaves as a typical avirulence gene. We demonstrate that PopP1 protein is secreted and that secretion of this protein requires a functional type III-secretion pathway. In contrast to the structural genes for other type III-secreted proteins identified in R. solanacearum, transcription of the popP1 gene is not coregulated with transcription of hrp genes but is constitutive. |
| first_indexed | 2024-04-13T03:15:45Z |
| format | Article |
| id | doaj.art-d681878588784538ad7f11e2f2fc06df |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T03:15:45Z |
| publishDate | 2002-10-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-d681878588784538ad7f11e2f2fc06df2022-12-22T03:04:56ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062002-10-0115101058106810.1094/MPMI.2002.15.10.1058PopP1, a New Member of the YopJ/AvrRxv Family of Type III Effector Proteins, Acts as a Host-Specificity Factor and Modulates Aggressiveness of Ralstonia solanacearumMuriel LavieEdwin ShillingtonCédric EguiluzNigel GrimsleyChristian BoucherA functional analysis of an 11-kb-long region of the genome of the plant-pathogenic bacterium Ralstonia solanacearum, previously identified as an alternative codon usage region (ACUR), reveals that it was probably acquired through horizontal gene transfer. This ACUR encodes an insertion sequence and eight potential proteins, one of which is partially homologous with a host-specificity factor from a plant-pathogenic Erwinia sp., and another, PopP1, which is homologous to members of the YopJ/AvrRxv family of type III-secreted bacterial effectors controlling interaction between bacteria and their hosts. The analysis of mutants affecting all except one of the genes identified in the ACUR showed that only the popP1-deficient strain had an altered phenotype in plant infection tests. This mutant strain became pathogenic to a Petunia line that is resistant to the wild-type strain. Therefore, popP1 behaves as a typical avirulence gene. We demonstrate that PopP1 protein is secreted and that secretion of this protein requires a functional type III-secretion pathway. In contrast to the structural genes for other type III-secreted proteins identified in R. solanacearum, transcription of the popP1 gene is not coregulated with transcription of hrp genes but is constitutive.https://apsjournals.apsnet.org/doi/10.1094/MPMI.2002.15.10.1058Arabidopsis thalianaHRtype III secretion pathwayvirulenceYopP |
| spellingShingle | Muriel Lavie Edwin Shillington Cédric Eguiluz Nigel Grimsley Christian Boucher PopP1, a New Member of the YopJ/AvrRxv Family of Type III Effector Proteins, Acts as a Host-Specificity Factor and Modulates Aggressiveness of Ralstonia solanacearum Molecular Plant-Microbe Interactions Arabidopsis thaliana HR type III secretion pathway virulence YopP |
| title | PopP1, a New Member of the YopJ/AvrRxv Family of Type III Effector Proteins, Acts as a Host-Specificity Factor and Modulates Aggressiveness of Ralstonia solanacearum |
| title_full | PopP1, a New Member of the YopJ/AvrRxv Family of Type III Effector Proteins, Acts as a Host-Specificity Factor and Modulates Aggressiveness of Ralstonia solanacearum |
| title_fullStr | PopP1, a New Member of the YopJ/AvrRxv Family of Type III Effector Proteins, Acts as a Host-Specificity Factor and Modulates Aggressiveness of Ralstonia solanacearum |
| title_full_unstemmed | PopP1, a New Member of the YopJ/AvrRxv Family of Type III Effector Proteins, Acts as a Host-Specificity Factor and Modulates Aggressiveness of Ralstonia solanacearum |
| title_short | PopP1, a New Member of the YopJ/AvrRxv Family of Type III Effector Proteins, Acts as a Host-Specificity Factor and Modulates Aggressiveness of Ralstonia solanacearum |
| title_sort | popp1 a new member of the yopj avrrxv family of type iii effector proteins acts as a host specificity factor and modulates aggressiveness of ralstonia solanacearum |
| topic | Arabidopsis thaliana HR type III secretion pathway virulence YopP |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2002.15.10.1058 |
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