Genome Mining of α-Pyrone Natural Products from Ascidian-Derived Fungus <i>Amphichorda</i><i>felina</i> SYSU-MS7908
Culturing ascidian-derived fungus <i>Amphichorda felina</i> SYSU-MS7908 under standard laboratory conditions mainly yielded meroterpenoid, and nonribosomal peptide-type natural products. We sequenced the genome of <i>Amphichorda felina</i> SYSU-MS7908 and found 56 biosyntheti...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/20/5/294 |
| _version_ | 1797498356253065216 |
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| author | Siwen Yuan Litong Chen Qilin Wu Minghua Jiang Heng Guo Zhibo Hu Senhua Chen Lan Liu Zhizeng Gao |
| author_facet | Siwen Yuan Litong Chen Qilin Wu Minghua Jiang Heng Guo Zhibo Hu Senhua Chen Lan Liu Zhizeng Gao |
| author_sort | Siwen Yuan |
| collection | DOAJ |
| description | Culturing ascidian-derived fungus <i>Amphichorda felina</i> SYSU-MS7908 under standard laboratory conditions mainly yielded meroterpenoid, and nonribosomal peptide-type natural products. We sequenced the genome of <i>Amphichorda felina</i> SYSU-MS7908 and found 56 biosynthetic gene clusters (BGCs) after bioinformatics analysis, suggesting that the majority of those BGCSs are silent. Here we report our genome mining effort on one cryptic BGC by heterologous expression in <i>Aspergillus oryzae</i> NSAR1, and the identification of two new α-pyrone derivatives, amphichopyrone A (<b>1</b>) and B (<b>2</b>), along with a known compound, udagawanone A (<b>3</b>). Anti-inflammatory activities were performed, and amphichopyrone A (<b>1</b>) and B (<b>2</b>) displayed potent anti-inflammatory activity by inhibiting nitric oxide (NO) production in RAW264.7 cells with IC<sub>50</sub> values 18.09 ± 4.83 and 7.18 ± 0.93 μM, respectively. |
| first_indexed | 2024-03-10T03:32:16Z |
| format | Article |
| id | doaj.art-d683e82a63ce41d3b7baa0e4b51745bc |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-10T03:32:16Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-d683e82a63ce41d3b7baa0e4b51745bc2023-11-23T11:54:06ZengMDPI AGMarine Drugs1660-33972022-04-0120529410.3390/md20050294Genome Mining of α-Pyrone Natural Products from Ascidian-Derived Fungus <i>Amphichorda</i><i>felina</i> SYSU-MS7908Siwen Yuan0Litong Chen1Qilin Wu2Minghua Jiang3Heng Guo4Zhibo Hu5Senhua Chen6Lan Liu7Zhizeng Gao8School of Maine Sciences, Sun Yat-sen University, Guangzhou 510006, ChinaSchool of Maine Sciences, Sun Yat-sen University, Guangzhou 510006, ChinaSchool of Maine Sciences, Sun Yat-sen University, Guangzhou 510006, ChinaSchool of Maine Sciences, Sun Yat-sen University, Guangzhou 510006, ChinaSchool of Maine Sciences, Sun Yat-sen University, Guangzhou 510006, ChinaSchool of Maine Sciences, Sun Yat-sen University, Guangzhou 510006, ChinaSchool of Maine Sciences, Sun Yat-sen University, Guangzhou 510006, ChinaSchool of Maine Sciences, Sun Yat-sen University, Guangzhou 510006, ChinaSchool of Maine Sciences, Sun Yat-sen University, Guangzhou 510006, ChinaCulturing ascidian-derived fungus <i>Amphichorda felina</i> SYSU-MS7908 under standard laboratory conditions mainly yielded meroterpenoid, and nonribosomal peptide-type natural products. We sequenced the genome of <i>Amphichorda felina</i> SYSU-MS7908 and found 56 biosynthetic gene clusters (BGCs) after bioinformatics analysis, suggesting that the majority of those BGCSs are silent. Here we report our genome mining effort on one cryptic BGC by heterologous expression in <i>Aspergillus oryzae</i> NSAR1, and the identification of two new α-pyrone derivatives, amphichopyrone A (<b>1</b>) and B (<b>2</b>), along with a known compound, udagawanone A (<b>3</b>). Anti-inflammatory activities were performed, and amphichopyrone A (<b>1</b>) and B (<b>2</b>) displayed potent anti-inflammatory activity by inhibiting nitric oxide (NO) production in RAW264.7 cells with IC<sub>50</sub> values 18.09 ± 4.83 and 7.18 ± 0.93 μM, respectively.https://www.mdpi.com/1660-3397/20/5/294genome miningα-pyrone natural productsheterologous expression<i>Aspergillus oryzae</i> NSAR1anti-inflammatory activity |
| spellingShingle | Siwen Yuan Litong Chen Qilin Wu Minghua Jiang Heng Guo Zhibo Hu Senhua Chen Lan Liu Zhizeng Gao Genome Mining of α-Pyrone Natural Products from Ascidian-Derived Fungus <i>Amphichorda</i><i>felina</i> SYSU-MS7908 Marine Drugs genome mining α-pyrone natural products heterologous expression <i>Aspergillus oryzae</i> NSAR1 anti-inflammatory activity |
| title | Genome Mining of α-Pyrone Natural Products from Ascidian-Derived Fungus <i>Amphichorda</i><i>felina</i> SYSU-MS7908 |
| title_full | Genome Mining of α-Pyrone Natural Products from Ascidian-Derived Fungus <i>Amphichorda</i><i>felina</i> SYSU-MS7908 |
| title_fullStr | Genome Mining of α-Pyrone Natural Products from Ascidian-Derived Fungus <i>Amphichorda</i><i>felina</i> SYSU-MS7908 |
| title_full_unstemmed | Genome Mining of α-Pyrone Natural Products from Ascidian-Derived Fungus <i>Amphichorda</i><i>felina</i> SYSU-MS7908 |
| title_short | Genome Mining of α-Pyrone Natural Products from Ascidian-Derived Fungus <i>Amphichorda</i><i>felina</i> SYSU-MS7908 |
| title_sort | genome mining of α pyrone natural products from ascidian derived fungus i amphichorda i i felina i sysu ms7908 |
| topic | genome mining α-pyrone natural products heterologous expression <i>Aspergillus oryzae</i> NSAR1 anti-inflammatory activity |
| url | https://www.mdpi.com/1660-3397/20/5/294 |
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