Understanding Cell Model Characteristics—RNA Expression Profiling in Primary and Immortalized Human Mesothelial Cells, and in Human Vein and Microvascular Endothelial Cells
In vitro studies are essential in pre-clinical research. While choice of cell lines is often driven by handling and cost-effectiveness, in-depth knowledge on specific characteristics is scant. Mesothelial cells, which interact with endothelial cells, are widely used in research, including cancer and...
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| Format: | Article |
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MDPI AG
2022-10-01
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| Online Access: | https://www.mdpi.com/2073-4409/11/19/3133 |
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| author | Iva Marinovic Maria Bartosova Rebecca Herzog Juan Manuel Sacnun Conghui Zhang Robin Hoogenboom Markus Unterwurzacher Thilo Hackert Aurelio A. Teleman Klaus Kratochwill Claus Peter Schmitt |
| author_facet | Iva Marinovic Maria Bartosova Rebecca Herzog Juan Manuel Sacnun Conghui Zhang Robin Hoogenboom Markus Unterwurzacher Thilo Hackert Aurelio A. Teleman Klaus Kratochwill Claus Peter Schmitt |
| author_sort | Iva Marinovic |
| collection | DOAJ |
| description | In vitro studies are essential in pre-clinical research. While choice of cell lines is often driven by handling and cost-effectiveness, in-depth knowledge on specific characteristics is scant. Mesothelial cells, which interact with endothelial cells, are widely used in research, including cancer and drug development, but have not been comprehensively profiled. We therefore performed RNA sequencing of polarized, primary peritoneal (HPMC) and immortalized pleural mesothelial cells (MeT-5A), and compared them to endothelial cells from umbilical vein (HUVEC) and cardiac capillaries (HCMEC). Seventy-seven per cent of 12,760 genes were shared between the 4 cell lines, 1003 were mesothelial and 969 were endothelial cell specific. The transcripts reflected major differences between HPMC and MeT-5A in DNA-related processes, extracellular matrix, migration, proliferation, adhesion, transport, growth factor- and immune response, and between HUVEC and HCMEC in DNA replication, extracellular matrix and adhesion organization. Highly variable shared genes were related to six clusters, cell tissue origin and immortalization, but also cell migration capacity, cell adhesion, regulation of angiogenesis and response to hypoxia. Distinct, cell type specific biological processes were further described by cellular component-, molecular function- and Reactome pathway analyses. We provide crucial information on specific features of the most frequently used mesothelial and endothelial cell lines, essential for appropriate use. |
| first_indexed | 2024-03-09T21:53:13Z |
| format | Article |
| id | doaj.art-d68416c2246943ac8f6b98d4c969705b |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T21:53:13Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-d68416c2246943ac8f6b98d4c969705b2023-11-23T20:03:25ZengMDPI AGCells2073-44092022-10-011119313310.3390/cells11193133Understanding Cell Model Characteristics—RNA Expression Profiling in Primary and Immortalized Human Mesothelial Cells, and in Human Vein and Microvascular Endothelial CellsIva Marinovic0Maria Bartosova1Rebecca Herzog2Juan Manuel Sacnun3Conghui Zhang4Robin Hoogenboom5Markus Unterwurzacher6Thilo Hackert7Aurelio A. Teleman8Klaus Kratochwill9Claus Peter Schmitt10Center for Pediatric and Adolescent Medicine, University of Heidelberg, 69120 Heidelberg, GermanyCenter for Pediatric and Adolescent Medicine, University of Heidelberg, 69120 Heidelberg, GermanyChristian Doppler Laboratory for Molecular Stress Research in Peritoneal Dialysis, Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, 1090 Vienna, AustriaChristian Doppler Laboratory for Molecular Stress Research in Peritoneal Dialysis, Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, 1090 Vienna, AustriaCenter for Pediatric and Adolescent Medicine, University of Heidelberg, 69120 Heidelberg, GermanyChristian Doppler Laboratory for Molecular Stress Research in Peritoneal Dialysis, Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, 1090 Vienna, AustriaChristian Doppler Laboratory for Molecular Stress Research in Peritoneal Dialysis, Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, 1090 Vienna, AustriaGeneral, Visceral and Transplantation Surgery, Heidelberg University, 69120 Heidelberg, GermanyGerman Cancer Research Center (DKFZ), 69120 Heidelberg, GermanyChristian Doppler Laboratory for Molecular Stress Research in Peritoneal Dialysis, Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, 1090 Vienna, AustriaCenter for Pediatric and Adolescent Medicine, University of Heidelberg, 69120 Heidelberg, GermanyIn vitro studies are essential in pre-clinical research. While choice of cell lines is often driven by handling and cost-effectiveness, in-depth knowledge on specific characteristics is scant. Mesothelial cells, which interact with endothelial cells, are widely used in research, including cancer and drug development, but have not been comprehensively profiled. We therefore performed RNA sequencing of polarized, primary peritoneal (HPMC) and immortalized pleural mesothelial cells (MeT-5A), and compared them to endothelial cells from umbilical vein (HUVEC) and cardiac capillaries (HCMEC). Seventy-seven per cent of 12,760 genes were shared between the 4 cell lines, 1003 were mesothelial and 969 were endothelial cell specific. The transcripts reflected major differences between HPMC and MeT-5A in DNA-related processes, extracellular matrix, migration, proliferation, adhesion, transport, growth factor- and immune response, and between HUVEC and HCMEC in DNA replication, extracellular matrix and adhesion organization. Highly variable shared genes were related to six clusters, cell tissue origin and immortalization, but also cell migration capacity, cell adhesion, regulation of angiogenesis and response to hypoxia. Distinct, cell type specific biological processes were further described by cellular component-, molecular function- and Reactome pathway analyses. We provide crucial information on specific features of the most frequently used mesothelial and endothelial cell lines, essential for appropriate use.https://www.mdpi.com/2073-4409/11/19/3133mesotheliumendotheliumRNA sequencingin vitrocell models |
| spellingShingle | Iva Marinovic Maria Bartosova Rebecca Herzog Juan Manuel Sacnun Conghui Zhang Robin Hoogenboom Markus Unterwurzacher Thilo Hackert Aurelio A. Teleman Klaus Kratochwill Claus Peter Schmitt Understanding Cell Model Characteristics—RNA Expression Profiling in Primary and Immortalized Human Mesothelial Cells, and in Human Vein and Microvascular Endothelial Cells Cells mesothelium endothelium RNA sequencing in vitro cell models |
| title | Understanding Cell Model Characteristics—RNA Expression Profiling in Primary and Immortalized Human Mesothelial Cells, and in Human Vein and Microvascular Endothelial Cells |
| title_full | Understanding Cell Model Characteristics—RNA Expression Profiling in Primary and Immortalized Human Mesothelial Cells, and in Human Vein and Microvascular Endothelial Cells |
| title_fullStr | Understanding Cell Model Characteristics—RNA Expression Profiling in Primary and Immortalized Human Mesothelial Cells, and in Human Vein and Microvascular Endothelial Cells |
| title_full_unstemmed | Understanding Cell Model Characteristics—RNA Expression Profiling in Primary and Immortalized Human Mesothelial Cells, and in Human Vein and Microvascular Endothelial Cells |
| title_short | Understanding Cell Model Characteristics—RNA Expression Profiling in Primary and Immortalized Human Mesothelial Cells, and in Human Vein and Microvascular Endothelial Cells |
| title_sort | understanding cell model characteristics rna expression profiling in primary and immortalized human mesothelial cells and in human vein and microvascular endothelial cells |
| topic | mesothelium endothelium RNA sequencing in vitro cell models |
| url | https://www.mdpi.com/2073-4409/11/19/3133 |
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