Elevated temperature during slicing enhances acute slice preparation quality
We demonstrate that brain dissection and slicing using solutions warmed to near-physiological temperature (~ +34˚C), greatly enhance slice quality without affecting intrinsic electrophysiological properties of the neurons. Improved slice quality is seen not only when using young (< 1 month),...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2013-04-01
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| Series: | Frontiers in Cellular Neuroscience |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fncel.2013.00048/full |