The Halotolerant Rhizobacterium—<em>Pseudomonas koreensis</em> MU2 Enhances Inorganic Silicon and Phosphorus Use Efficiency and Augments Salt Stress Tolerance in Soybean (<em>Glycine max</em> L.)
Optimizing nutrient usage in plants is vital for a sustainable yield under biotic and abiotic stresses. Since silicon and phosphorus are considered key elements for plant growth, this study assessed the efficient supplementation strategy of silicon and phosphorus in soybean plants under salt stress...
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MDPI AG
2020-08-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/9/1256 |
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| author | Arjun Adhikari Muhammad Aaqil Khan Ko-Eun Lee Sang-Mo Kang Sanjeev Kumar Dhungana Narayan Bhusal In-Jung Lee |
| author_facet | Arjun Adhikari Muhammad Aaqil Khan Ko-Eun Lee Sang-Mo Kang Sanjeev Kumar Dhungana Narayan Bhusal In-Jung Lee |
| author_sort | Arjun Adhikari |
| collection | DOAJ |
| description | Optimizing nutrient usage in plants is vital for a sustainable yield under biotic and abiotic stresses. Since silicon and phosphorus are considered key elements for plant growth, this study assessed the efficient supplementation strategy of silicon and phosphorus in soybean plants under salt stress through inoculation using the rhizospheric strain—<i>Pseudomonas koreensis</i> MU2. The screening analysis of MU2 showed its high salt-tolerant potential, which solubilizes both silicate and phosphate. The isolate, MU2 produced gibberellic acid (GA<sub>1</sub>, GA<sub>3</sub>) and organic acids (malic acid, citric acid, acetic acid, and tartaric acid) in pure culture under both normal and salt-stressed conditions. The combined application of MU2, silicon, and phosphorus significantly improved silicon and phosphorus uptake, reduced Na<sup>+</sup> ion influx by 70%, and enhanced K<sup>+</sup> uptake by 46% in the shoots of soybean plants grown under salt-stress conditions. MU2 inoculation upregulated the salt-resistant genes <i>GmST1</i>, <i>GmSALT3</i>, and <i>GmAKT2</i>, which significantly reduced the endogenous hormones abscisic acid and jasmonic acid while, it enhanced the salicylic acid content of soybean. In addition, MU2 inoculation strengthened the host’s antioxidant system through the reduction of lipid peroxidation and proline while, it enhanced the reduced glutathione content. Moreover, MU2 inoculation promoted root and shoot length, plant biomass, and the chlorophyll content of soybean plants. These findings suggest that MU2 could be a potential biofertilizer catalyst for the amplification of the use efficiency of silicon and phosphorus fertilizers to mitigate salt stress. |
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| language | English |
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| publishDate | 2020-08-01 |
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| spelling | doaj.art-d6853a4f59e843a1b9a714649d3a34522023-11-20T10:37:18ZengMDPI AGMicroorganisms2076-26072020-08-0189125610.3390/microorganisms8091256The Halotolerant Rhizobacterium—<em>Pseudomonas koreensis</em> MU2 Enhances Inorganic Silicon and Phosphorus Use Efficiency and Augments Salt Stress Tolerance in Soybean (<em>Glycine max</em> L.)Arjun Adhikari0Muhammad Aaqil Khan1Ko-Eun Lee2Sang-Mo Kang3Sanjeev Kumar Dhungana4Narayan Bhusal5In-Jung Lee6School of Applied Biosciences, Kyungpook National University, Daegu 41566, KoreaSchool of Applied Biosciences, Kyungpook National University, Daegu 41566, KoreaSchool of Applied Biosciences, Kyungpook National University, Daegu 41566, KoreaSchool of Applied Biosciences, Kyungpook National University, Daegu 41566, KoreaDepartment of Southern Area Crop Science, National Institute of Crop Science, Rural Development Administration, Miryang 50424, KoreaDepartment of Forest Sciences, Seoul National University, Seoul 08826, KoreaSchool of Applied Biosciences, Kyungpook National University, Daegu 41566, KoreaOptimizing nutrient usage in plants is vital for a sustainable yield under biotic and abiotic stresses. Since silicon and phosphorus are considered key elements for plant growth, this study assessed the efficient supplementation strategy of silicon and phosphorus in soybean plants under salt stress through inoculation using the rhizospheric strain—<i>Pseudomonas koreensis</i> MU2. The screening analysis of MU2 showed its high salt-tolerant potential, which solubilizes both silicate and phosphate. The isolate, MU2 produced gibberellic acid (GA<sub>1</sub>, GA<sub>3</sub>) and organic acids (malic acid, citric acid, acetic acid, and tartaric acid) in pure culture under both normal and salt-stressed conditions. The combined application of MU2, silicon, and phosphorus significantly improved silicon and phosphorus uptake, reduced Na<sup>+</sup> ion influx by 70%, and enhanced K<sup>+</sup> uptake by 46% in the shoots of soybean plants grown under salt-stress conditions. MU2 inoculation upregulated the salt-resistant genes <i>GmST1</i>, <i>GmSALT3</i>, and <i>GmAKT2</i>, which significantly reduced the endogenous hormones abscisic acid and jasmonic acid while, it enhanced the salicylic acid content of soybean. In addition, MU2 inoculation strengthened the host’s antioxidant system through the reduction of lipid peroxidation and proline while, it enhanced the reduced glutathione content. Moreover, MU2 inoculation promoted root and shoot length, plant biomass, and the chlorophyll content of soybean plants. These findings suggest that MU2 could be a potential biofertilizer catalyst for the amplification of the use efficiency of silicon and phosphorus fertilizers to mitigate salt stress.https://www.mdpi.com/2076-2607/8/9/1256siliconphosphorussolubilization<i>P. koreensis</i> MU2soybeansalinity |
| spellingShingle | Arjun Adhikari Muhammad Aaqil Khan Ko-Eun Lee Sang-Mo Kang Sanjeev Kumar Dhungana Narayan Bhusal In-Jung Lee The Halotolerant Rhizobacterium—<em>Pseudomonas koreensis</em> MU2 Enhances Inorganic Silicon and Phosphorus Use Efficiency and Augments Salt Stress Tolerance in Soybean (<em>Glycine max</em> L.) Microorganisms silicon phosphorus solubilization <i>P. koreensis</i> MU2 soybean salinity |
| title | The Halotolerant Rhizobacterium—<em>Pseudomonas koreensis</em> MU2 Enhances Inorganic Silicon and Phosphorus Use Efficiency and Augments Salt Stress Tolerance in Soybean (<em>Glycine max</em> L.) |
| title_full | The Halotolerant Rhizobacterium—<em>Pseudomonas koreensis</em> MU2 Enhances Inorganic Silicon and Phosphorus Use Efficiency and Augments Salt Stress Tolerance in Soybean (<em>Glycine max</em> L.) |
| title_fullStr | The Halotolerant Rhizobacterium—<em>Pseudomonas koreensis</em> MU2 Enhances Inorganic Silicon and Phosphorus Use Efficiency and Augments Salt Stress Tolerance in Soybean (<em>Glycine max</em> L.) |
| title_full_unstemmed | The Halotolerant Rhizobacterium—<em>Pseudomonas koreensis</em> MU2 Enhances Inorganic Silicon and Phosphorus Use Efficiency and Augments Salt Stress Tolerance in Soybean (<em>Glycine max</em> L.) |
| title_short | The Halotolerant Rhizobacterium—<em>Pseudomonas koreensis</em> MU2 Enhances Inorganic Silicon and Phosphorus Use Efficiency and Augments Salt Stress Tolerance in Soybean (<em>Glycine max</em> L.) |
| title_sort | halotolerant rhizobacterium em pseudomonas koreensis em mu2 enhances inorganic silicon and phosphorus use efficiency and augments salt stress tolerance in soybean em glycine max em l |
| topic | silicon phosphorus solubilization <i>P. koreensis</i> MU2 soybean salinity |
| url | https://www.mdpi.com/2076-2607/8/9/1256 |
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