Species Specific Detection of Methicillin-resistant Staphylococcus aureus (MRSA) by Multiplex PCR
Introduction: Staphylococcus aureus is one of the pathogens that is a major agent of infectious diseases. So detection, control and treatment of this pathogen is very important. The objective of this study is setting up a fast method to detect methicillin resistant Staphylococcus aureus (MRSA) for b...
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| Format: | Article |
| Language: | fas |
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Isfahan University of Medical Sciences
2013-01-01
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| Series: | مجله دانشکده پزشکی اصفهان |
| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/2175 |
| _version_ | 1827836188019392512 |
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| author | Mehdi Haseli Ali Ramazani Alireza Khaleghi Khorram Laleh Mehrad |
| author_facet | Mehdi Haseli Ali Ramazani Alireza Khaleghi Khorram Laleh Mehrad |
| author_sort | Mehdi Haseli |
| collection | DOAJ |
| description | Introduction: Staphylococcus aureus is one of the pathogens that is a major agent of infectious diseases. So detection, control and treatment of this pathogen is very important. The objective of this study is setting up a fast method to detect methicillin resistant Staphylococcus aureus (MRSA) for better management of diseases related to this pathogen.
Materials and methods: In this study 225 sample collected form staff of University hospitals of Zanjan province. After detection and confirmation of bacteria by conventional and biochemical methods, antimicrobial susceptibility was tested by disk diffusion (Kirby-Bauer) method. After DNA extraction by boiling and phenol – chloroform method, Sa442, mecA and femA genes were detected by multiplex PCR and PCR products were analyzed by electrophoresis (70V, 30 min) in gels composed of 1.5% (w/v) agarose stained with SyberGreen.
Result: 208 out of 225 samples made colony in bacterial culture medium. 203/208 isolates were gram positive; 201/208 isolates were catalase positive and 71/208 isolates were coagulase positive. Most of the isolates were resistant to oxacillin and penicillin. By optimization of PCR conditions, Sa442, mecA and femA genes were detected by multiplex PCR simultaneously in single reaction tube.
Discussion: Multiplex PCR can detect rapidly and selectively methicillin resistant Staphylococcus aureus in comparison with disk diffusion method. |
| first_indexed | 2024-03-12T06:22:23Z |
| format | Article |
| id | doaj.art-d68c53e8010a49b8822e731b657e045d |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T06:22:23Z |
| publishDate | 2013-01-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-d68c53e8010a49b8822e731b657e045d2023-09-03T02:11:05ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2013-01-01302131199Species Specific Detection of Methicillin-resistant Staphylococcus aureus (MRSA) by Multiplex PCRMehdi Haseli0Ali Ramazani1Alireza Khaleghi Khorram2Laleh Mehrad3PharmD Student, Department of Biotechnology, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, IranAssistant Professor, Department of Biotechnology, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, IranDepartment of Microbiology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, IranMSc Student, Department of Microbiology, Islamic Azad University, Science and Research Branch, Arak, IranIntroduction: Staphylococcus aureus is one of the pathogens that is a major agent of infectious diseases. So detection, control and treatment of this pathogen is very important. The objective of this study is setting up a fast method to detect methicillin resistant Staphylococcus aureus (MRSA) for better management of diseases related to this pathogen. Materials and methods: In this study 225 sample collected form staff of University hospitals of Zanjan province. After detection and confirmation of bacteria by conventional and biochemical methods, antimicrobial susceptibility was tested by disk diffusion (Kirby-Bauer) method. After DNA extraction by boiling and phenol – chloroform method, Sa442, mecA and femA genes were detected by multiplex PCR and PCR products were analyzed by electrophoresis (70V, 30 min) in gels composed of 1.5% (w/v) agarose stained with SyberGreen. Result: 208 out of 225 samples made colony in bacterial culture medium. 203/208 isolates were gram positive; 201/208 isolates were catalase positive and 71/208 isolates were coagulase positive. Most of the isolates were resistant to oxacillin and penicillin. By optimization of PCR conditions, Sa442, mecA and femA genes were detected by multiplex PCR simultaneously in single reaction tube. Discussion: Multiplex PCR can detect rapidly and selectively methicillin resistant Staphylococcus aureus in comparison with disk diffusion method.http://jims.mui.ac.ir/index.php/jims/article/view/2175 |
| spellingShingle | Mehdi Haseli Ali Ramazani Alireza Khaleghi Khorram Laleh Mehrad Species Specific Detection of Methicillin-resistant Staphylococcus aureus (MRSA) by Multiplex PCR مجله دانشکده پزشکی اصفهان |
| title | Species Specific Detection of Methicillin-resistant Staphylococcus aureus (MRSA) by Multiplex PCR |
| title_full | Species Specific Detection of Methicillin-resistant Staphylococcus aureus (MRSA) by Multiplex PCR |
| title_fullStr | Species Specific Detection of Methicillin-resistant Staphylococcus aureus (MRSA) by Multiplex PCR |
| title_full_unstemmed | Species Specific Detection of Methicillin-resistant Staphylococcus aureus (MRSA) by Multiplex PCR |
| title_short | Species Specific Detection of Methicillin-resistant Staphylococcus aureus (MRSA) by Multiplex PCR |
| title_sort | species specific detection of methicillin resistant staphylococcus aureus mrsa by multiplex pcr |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/2175 |
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