The autophagic inducer and inhibitor display different activities on the meiotic and developmental competencies of porcine oocytes derived from small and medium follicles
This study aimed to examine the effect of rapamycin (autophagy inducer) and 3-methyladenine (3-MA, autophagy inhibitor) on the meiotic and developmental competencies of porcine oocytes derived from medium follicles (MF, 3–6 mm in diameter) and small follicles (SF, 1–2 mm in diameter) during in vitro...
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| Format: | Article |
| Language: | English |
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The Society for Reproduction and Development
2019-11-01
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| Series: | The Journal of Reproduction and Development |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jrd/65/6/65_2019-112/_pdf/-char/en |
| _version_ | 1827752484167221248 |
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| author | Chiyuki KOHATA-ONO Takuya WAKAI Hiroaki FUNAHASHI |
| author_facet | Chiyuki KOHATA-ONO Takuya WAKAI Hiroaki FUNAHASHI |
| author_sort | Chiyuki KOHATA-ONO |
| collection | DOAJ |
| description | This study aimed to examine the effect of rapamycin (autophagy inducer) and 3-methyladenine (3-MA, autophagy inhibitor) on the meiotic and developmental competencies of porcine oocytes derived from medium follicles (MF, 3–6 mm in diameter) and small follicles (SF, 1–2 mm in diameter) during in vitro maturation (IVM) process. The presence of 1 nM but not 10 nM rapamycin significantly increased the maturation rate of MF-derived oocytes (P < 0.05). However, the maturation rate of SF-derived oocytes was not affected by rapamycin at both concentrations (1 nM and 10 nM). The maturation rate of MF-derived oocytes decreased significantly (P < 0.05) in the presence of 0.2 mM but not 2 mM 3-MA than non-supplemented control. In contrast, in SF-derived oocytes, 3-MA at both 0.2 and 2 mM concentrations did not affect the maturation rates. The presence of 1 nM rapamycin significantly increased the blastocyst formation rate of MF-derived mature oocytes following parthenogenetic activation (P < 0.05). However, the blastocyst formation rate of SF-derived mature oocytes was not affected by the presence of rapamycin. The presence of 3-MA significantly reduced the blastocyst formation rate of MF-derived mature oocytes but did not change that of SF-derived oocytes. In conclusion, our study results show differences in activity of the autophagy inducer and inhibitor on the meiotic and developmental competencies of MF- and SF-derived porcine oocytes. |
| first_indexed | 2024-03-11T07:17:03Z |
| format | Article |
| id | doaj.art-d68f62b3bd0d44bebf17baa58ae88d1a |
| institution | Directory Open Access Journal |
| issn | 0916-8818 1348-4400 |
| language | English |
| last_indexed | 2024-03-11T07:17:03Z |
| publishDate | 2019-11-01 |
| publisher | The Society for Reproduction and Development |
| record_format | Article |
| series | The Journal of Reproduction and Development |
| spelling | doaj.art-d68f62b3bd0d44bebf17baa58ae88d1a2023-11-17T08:13:46ZengThe Society for Reproduction and DevelopmentThe Journal of Reproduction and Development0916-88181348-44002019-11-0165652753210.1262/jrd.2019-112jrdThe autophagic inducer and inhibitor display different activities on the meiotic and developmental competencies of porcine oocytes derived from small and medium folliclesChiyuki KOHATA-ONO0Takuya WAKAI1Hiroaki FUNAHASHI2Department of Animal Science, Graduate of Environmental and Life Science, Okayama University, Okayama 700-8530, JapanDepartment of Animal Science, Graduate of Environmental and Life Science, Okayama University, Okayama 700-8530, JapanDepartment of Animal Science, Graduate of Environmental and Life Science, Okayama University, Okayama 700-8530, JapanThis study aimed to examine the effect of rapamycin (autophagy inducer) and 3-methyladenine (3-MA, autophagy inhibitor) on the meiotic and developmental competencies of porcine oocytes derived from medium follicles (MF, 3–6 mm in diameter) and small follicles (SF, 1–2 mm in diameter) during in vitro maturation (IVM) process. The presence of 1 nM but not 10 nM rapamycin significantly increased the maturation rate of MF-derived oocytes (P < 0.05). However, the maturation rate of SF-derived oocytes was not affected by rapamycin at both concentrations (1 nM and 10 nM). The maturation rate of MF-derived oocytes decreased significantly (P < 0.05) in the presence of 0.2 mM but not 2 mM 3-MA than non-supplemented control. In contrast, in SF-derived oocytes, 3-MA at both 0.2 and 2 mM concentrations did not affect the maturation rates. The presence of 1 nM rapamycin significantly increased the blastocyst formation rate of MF-derived mature oocytes following parthenogenetic activation (P < 0.05). However, the blastocyst formation rate of SF-derived mature oocytes was not affected by the presence of rapamycin. The presence of 3-MA significantly reduced the blastocyst formation rate of MF-derived mature oocytes but did not change that of SF-derived oocytes. In conclusion, our study results show differences in activity of the autophagy inducer and inhibitor on the meiotic and developmental competencies of MF- and SF-derived porcine oocytes.https://www.jstage.jst.go.jp/article/jrd/65/6/65_2019-112/_pdf/-char/enautophagydevelopmental competencein vitro maturationoocytesporcine |
| spellingShingle | Chiyuki KOHATA-ONO Takuya WAKAI Hiroaki FUNAHASHI The autophagic inducer and inhibitor display different activities on the meiotic and developmental competencies of porcine oocytes derived from small and medium follicles The Journal of Reproduction and Development autophagy developmental competence in vitro maturation oocytes porcine |
| title | The autophagic inducer and inhibitor display different activities on the meiotic and developmental competencies of porcine oocytes derived from small and medium follicles |
| title_full | The autophagic inducer and inhibitor display different activities on the meiotic and developmental competencies of porcine oocytes derived from small and medium follicles |
| title_fullStr | The autophagic inducer and inhibitor display different activities on the meiotic and developmental competencies of porcine oocytes derived from small and medium follicles |
| title_full_unstemmed | The autophagic inducer and inhibitor display different activities on the meiotic and developmental competencies of porcine oocytes derived from small and medium follicles |
| title_short | The autophagic inducer and inhibitor display different activities on the meiotic and developmental competencies of porcine oocytes derived from small and medium follicles |
| title_sort | autophagic inducer and inhibitor display different activities on the meiotic and developmental competencies of porcine oocytes derived from small and medium follicles |
| topic | autophagy developmental competence in vitro maturation oocytes porcine |
| url | https://www.jstage.jst.go.jp/article/jrd/65/6/65_2019-112/_pdf/-char/en |
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