Calprotectin mRNA (MRP8/MRP14) expression in neutrophils of periodontitis patients with type 2 diabetes mellitus
<span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Background: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;&quo...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universitas Airlangga
2009-09-01
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| Series: | Dental Journal |
| Subjects: | |
| Online Access: | http://e-journal.unair.ac.id/index.php/MKG/article/view/951 |
| _version_ | 1819290883315466240 |
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| author | Ahmad Syaify Marsetyawan HNES Sudibyo Sudibyo Suryono Suryono |
| author_facet | Ahmad Syaify Marsetyawan HNES Sudibyo Sudibyo Suryono Suryono |
| author_sort | Ahmad Syaify |
| collection | DOAJ |
| description | <span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Background: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Calprotectin, a major cytosolic protein of leukocytes, is detected in neutrophils and monocytes/machrophages. This </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>protein is known to be a marker for several inflammatory diseases including periodontitis. In type 2 diabetes mellitus patients, the </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>severity of periodontitis was strongly thought to be caused by decreasing of leukocytes function such as neutrophils. Previous research </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>found that the calprotectin level in serum of periodontitis patients with type 2 DM is higher than periodontits patients non DM. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Purpose: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>The aim of this study was to determine calprotectin mRNA (MRP8/MRP14) expression in human neutrophils of periodontitis patients </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>with type 2 diabetes mellitus. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Methods: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Neutrophils were isolated from the peripheral blood of periodontitis patients with uncontrolled </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>type 2 DM, controlled type 2 DM, and non DM. The expression of calprotectin mRNA (MRP8 and MRP14) were detected by RTPCR. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Result: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>The result showed that the value of mRNA calprotectin expression in DM patients were higher than non DM, and the </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>highest expression was on the uncontrolled type 2 DM. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Conclusion: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>The basal level of calprotectin mRNA MRP8/MRP14 expression </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>increased in neutrophil of periodontitis patient with type 2 DM compared non diabetic subjects. It was suggested that high basal level </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>of calprotectin mRNA has a role in the regulation of periodontitis severity with diabetes mellitus patients.</em></span></span></span></span></span></span></span></span></span><br style="font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px;" /></span></span></span></span></span></span></span></span></span> |
| first_indexed | 2024-12-24T03:29:49Z |
| format | Article |
| id | doaj.art-d692fcbbaa794525ae1d54fd59ed0e1b |
| institution | Directory Open Access Journal |
| issn | 1978-3728 2442-9740 |
| language | English |
| last_indexed | 2024-12-24T03:29:49Z |
| publishDate | 2009-09-01 |
| publisher | Universitas Airlangga |
| record_format | Article |
| series | Dental Journal |
| spelling | doaj.art-d692fcbbaa794525ae1d54fd59ed0e1b2022-12-21T17:17:15ZengUniversitas AirlanggaDental Journal1978-37282442-97402009-09-0142313013310.20473/j.djmkg.v42.i3.p130-133793Calprotectin mRNA (MRP8/MRP14) expression in neutrophils of periodontitis patients with type 2 diabetes mellitusAhmad Syaify0Marsetyawan HNES1Sudibyo Sudibyo2Suryono Suryono3Departement of Periodontology, Faculty of Dentistry, Universitas Gadjah MadaDepartment of Immunology, Faculty of Medicine, Universitas Gadjah MadaDepartement of Periodontology, Faculty of Dentistry, Universitas Gadjah MadaDepartement of Periodontology, Faculty of Dentistry, Universitas Gadjah Mada<span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Background: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Calprotectin, a major cytosolic protein of leukocytes, is detected in neutrophils and monocytes/machrophages. This </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>protein is known to be a marker for several inflammatory diseases including periodontitis. In type 2 diabetes mellitus patients, the </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>severity of periodontitis was strongly thought to be caused by decreasing of leukocytes function such as neutrophils. Previous research </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>found that the calprotectin level in serum of periodontitis patients with type 2 DM is higher than periodontits patients non DM. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Purpose: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>The aim of this study was to determine calprotectin mRNA (MRP8/MRP14) expression in human neutrophils of periodontitis patients </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>with type 2 diabetes mellitus. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Methods: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Neutrophils were isolated from the peripheral blood of periodontitis patients with uncontrolled </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>type 2 DM, controlled type 2 DM, and non DM. The expression of calprotectin mRNA (MRP8 and MRP14) were detected by RTPCR. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Result: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>The result showed that the value of mRNA calprotectin expression in DM patients were higher than non DM, and the </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>highest expression was on the uncontrolled type 2 DM. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Conclusion: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>The basal level of calprotectin mRNA MRP8/MRP14 expression </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>increased in neutrophil of periodontitis patient with type 2 DM compared non diabetic subjects. It was suggested that high basal level </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>of calprotectin mRNA has a role in the regulation of periodontitis severity with diabetes mellitus patients.</em></span></span></span></span></span></span></span></span></span><br style="font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px;" /></span></span></span></span></span></span></span></span></span>http://e-journal.unair.ac.id/index.php/MKG/article/view/951calprotectin mRNAperiodontitistype2 diabetes mellitus |
| spellingShingle | Ahmad Syaify Marsetyawan HNES Sudibyo Sudibyo Suryono Suryono Calprotectin mRNA (MRP8/MRP14) expression in neutrophils of periodontitis patients with type 2 diabetes mellitus Dental Journal calprotectin mRNA periodontitis type2 diabetes mellitus |
| title | Calprotectin mRNA (MRP8/MRP14) expression in neutrophils of periodontitis patients with type 2 diabetes mellitus |
| title_full | Calprotectin mRNA (MRP8/MRP14) expression in neutrophils of periodontitis patients with type 2 diabetes mellitus |
| title_fullStr | Calprotectin mRNA (MRP8/MRP14) expression in neutrophils of periodontitis patients with type 2 diabetes mellitus |
| title_full_unstemmed | Calprotectin mRNA (MRP8/MRP14) expression in neutrophils of periodontitis patients with type 2 diabetes mellitus |
| title_short | Calprotectin mRNA (MRP8/MRP14) expression in neutrophils of periodontitis patients with type 2 diabetes mellitus |
| title_sort | calprotectin mrna mrp8 mrp14 expression in neutrophils of periodontitis patients with type 2 diabetes mellitus |
| topic | calprotectin mRNA periodontitis type2 diabetes mellitus |
| url | http://e-journal.unair.ac.id/index.php/MKG/article/view/951 |
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