The effect of dental caries and restorative biomaterials on IL-1 β and TNF-α levels in the gingival crevicular fluid
Background/Aim. In the spirit of personalized medicine, determining caries biomarkers in the saliva and gingival crevicular fluid (GCF) attracts great attention in the current dental re-search. The concentration of GCF cytokines is illustrative in depicting the processes in tooth structures. Their r...
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| Format: | Article |
| Language: | English |
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Military Health Department, Ministry of Defance, Serbia
2021-01-01
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| Series: | Vojnosanitetski Pregled |
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| Online Access: | http://www.doiserbia.nb.rs/img/doi/0042-8450/2021/0042-84501900038S.pdf |
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| author | Stefanović Vladimir Taso Ervin Kanjevac Tatjana Abazović Džihan Rakić Mia Petković-Ćurčin Aleksandra Acović Aleksandar Vojvodić Danilo |
| author_facet | Stefanović Vladimir Taso Ervin Kanjevac Tatjana Abazović Džihan Rakić Mia Petković-Ćurčin Aleksandra Acović Aleksandar Vojvodić Danilo |
| author_sort | Stefanović Vladimir |
| collection | DOAJ |
| description | Background/Aim. In the spirit of personalized medicine, determining caries biomarkers in the saliva and gingival crevicular fluid (GCF) attracts great attention in the current dental re-search. The concentration of GCF cytokines is illustrative in depicting the processes in tooth structures. Their relevance must be inspected with aspects of tooth position and caries lesion level. Different impacts of dental restoration materials on GCF IL-1β and TNF-α could be used as a parameter for estimating local inflammation. This paper aimed to estimate the concentrations of the proinflammatory cytokines (IL-1β and TNF-α) in the GCF and to correlate them with caries extension, tooth position, and different restorative biomaterials. Methods. GCF samples were collected from 90 periodontally healthy patients demonstrating at least one tooth with proximal caries and one intact tooth, at the baseline, 7 and 30-days post-treatment. The biomarkers’ profile was investigated in relation to different levels of caries extension (superficial, pulpitis, gangrenous, root affection), defect size, and restorative biomaterial. Results. Before therapy, caries level was significantly associated with GCF IL-1β concentration, demonstrating the lowest level in gangrenous (C4) and superficial caries (C2). Thirty days after therapy, root affection (C5) was characterized by the highest IL-1β concentration. Different dental fillings showed various GCF cytokine changes. CPC induced a significant IL-1β increase in more than 70% of treated patients. Caries lesion size was insignificantly associated with GCF levels of these proinflammatory cytokines, where larger defects were followed by an average cytokine increase. Considering the tooth position be-fore therapy, IL-1β had the highest level in GCF samples from caries-affected canines and second molars, while TNF-α showed the highest levels from canines GCF. Dental restoration induced cytokine increase in canines (IL-1β and TNF-α), 1st and 2nd molars GCF (IL-1β). Conclusion. Inflammation intensity of tooth structures was directly reflected in IL-1β and TNF-α concentrations. Dental restoration significantly affects IL-1β and TNF-α levels, depending on the used dental filling-type material. The profile of these cytokines varied in GCF samples of the tooth with different anatomical positions, where canines and molars demonstrated the highest level. An increase of these proinflammatory cytokines in the absence of any symptomatic manifestation of the inflammatory response can be considered as a possible tooth reparation parameter. |
| first_indexed | 2024-12-15T00:22:20Z |
| format | Article |
| id | doaj.art-d69357dd8c9c4ab8990e5c47954755e1 |
| institution | Directory Open Access Journal |
| issn | 0042-8450 2406-0720 |
| language | English |
| last_indexed | 2024-12-15T00:22:20Z |
| publishDate | 2021-01-01 |
| publisher | Military Health Department, Ministry of Defance, Serbia |
| record_format | Article |
| series | Vojnosanitetski Pregled |
| spelling | doaj.art-d69357dd8c9c4ab8990e5c47954755e12022-12-21T22:42:17ZengMilitary Health Department, Ministry of Defance, SerbiaVojnosanitetski Pregled0042-84502406-07202021-01-01781627110.2298/VSP181116038S0042-84501900038SThe effect of dental caries and restorative biomaterials on IL-1 β and TNF-α levels in the gingival crevicular fluidStefanović Vladimir0Taso Ervin1Kanjevac Tatjana2https://orcid.org/0000-0002-7524-040XAbazović Džihan3Rakić Mia4https://orcid.org/0000-0001-7093-7956Petković-Ćurčin Aleksandra5Acović Aleksandar6https://orcid.org/0000-0002-6573-6911Vojvodić Danilo7https://orcid.org/0000-0001-9308-3076Military Medical Academy, Clinic for Stomatology, Belgrade, SerbiaMilitary Medical Academy, Clinic for Stomatology, Belgrade, SerbiaUniversity of Kragujevac, Faculty of Medical Sciences, Department of Dentistry, Kragujevac, SerbiaClinical Center, Emergency Medicine Center Montenegro, Podgorica, MontenegroUniversity of Belgrade, Institute for Biological Research “Siniša Stanković”, Belgrade, SerbiaMilitary Medical Academy, Institute for Medical Research, Belgrade, SerbiaUniversity of Kragujevac, Faculty of Medical Sciences, Department of Dentistry, Kragujevac, SerbiaMilitary Medical Academy, Institute for Medical Research, Belgrade, Serbia + University of Defence, Faculty of Medicine, Military Medical Academy, Belgrade, Serbia Background/Aim. In the spirit of personalized medicine, determining caries biomarkers in the saliva and gingival crevicular fluid (GCF) attracts great attention in the current dental re-search. The concentration of GCF cytokines is illustrative in depicting the processes in tooth structures. Their relevance must be inspected with aspects of tooth position and caries lesion level. Different impacts of dental restoration materials on GCF IL-1β and TNF-α could be used as a parameter for estimating local inflammation. This paper aimed to estimate the concentrations of the proinflammatory cytokines (IL-1β and TNF-α) in the GCF and to correlate them with caries extension, tooth position, and different restorative biomaterials. Methods. GCF samples were collected from 90 periodontally healthy patients demonstrating at least one tooth with proximal caries and one intact tooth, at the baseline, 7 and 30-days post-treatment. The biomarkers’ profile was investigated in relation to different levels of caries extension (superficial, pulpitis, gangrenous, root affection), defect size, and restorative biomaterial. Results. Before therapy, caries level was significantly associated with GCF IL-1β concentration, demonstrating the lowest level in gangrenous (C4) and superficial caries (C2). Thirty days after therapy, root affection (C5) was characterized by the highest IL-1β concentration. Different dental fillings showed various GCF cytokine changes. CPC induced a significant IL-1β increase in more than 70% of treated patients. Caries lesion size was insignificantly associated with GCF levels of these proinflammatory cytokines, where larger defects were followed by an average cytokine increase. Considering the tooth position be-fore therapy, IL-1β had the highest level in GCF samples from caries-affected canines and second molars, while TNF-α showed the highest levels from canines GCF. Dental restoration induced cytokine increase in canines (IL-1β and TNF-α), 1st and 2nd molars GCF (IL-1β). Conclusion. Inflammation intensity of tooth structures was directly reflected in IL-1β and TNF-α concentrations. Dental restoration significantly affects IL-1β and TNF-α levels, depending on the used dental filling-type material. The profile of these cytokines varied in GCF samples of the tooth with different anatomical positions, where canines and molars demonstrated the highest level. An increase of these proinflammatory cytokines in the absence of any symptomatic manifestation of the inflammatory response can be considered as a possible tooth reparation parameter.http://www.doiserbia.nb.rs/img/doi/0042-8450/2021/0042-84501900038S.pdfdental cariesdental restoration, permanentdental materialsgingival crevicular fluidinterleukin-1tumor necrosis factor-alpha |
| spellingShingle | Stefanović Vladimir Taso Ervin Kanjevac Tatjana Abazović Džihan Rakić Mia Petković-Ćurčin Aleksandra Acović Aleksandar Vojvodić Danilo The effect of dental caries and restorative biomaterials on IL-1 β and TNF-α levels in the gingival crevicular fluid Vojnosanitetski Pregled dental caries dental restoration, permanent dental materials gingival crevicular fluid interleukin-1 tumor necrosis factor-alpha |
| title | The effect of dental caries and restorative biomaterials on IL-1 β and TNF-α levels in the gingival crevicular fluid |
| title_full | The effect of dental caries and restorative biomaterials on IL-1 β and TNF-α levels in the gingival crevicular fluid |
| title_fullStr | The effect of dental caries and restorative biomaterials on IL-1 β and TNF-α levels in the gingival crevicular fluid |
| title_full_unstemmed | The effect of dental caries and restorative biomaterials on IL-1 β and TNF-α levels in the gingival crevicular fluid |
| title_short | The effect of dental caries and restorative biomaterials on IL-1 β and TNF-α levels in the gingival crevicular fluid |
| title_sort | effect of dental caries and restorative biomaterials on il 1 β and tnf α levels in the gingival crevicular fluid |
| topic | dental caries dental restoration, permanent dental materials gingival crevicular fluid interleukin-1 tumor necrosis factor-alpha |
| url | http://www.doiserbia.nb.rs/img/doi/0042-8450/2021/0042-84501900038S.pdf |
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