Fecal Cortisol and Progesterone Concentrations in Post Partus of Etawah Crossbreed Goat
Progesterone (P4) is a dominant hormone during pregnancy. In the later stage of pregnancy, the stress hormone particularly cortisol (C) may increase for initiating the parturition process as a consequence of fetal stress. This study was a preliminary study to compare the concentration of P4 and C in...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
EDP Sciences
2020-01-01
|
Series: | E3S Web of Conferences |
Subjects: | |
Online Access: | https://www.e3s-conferences.org/articles/e3sconf/pdf/2020/11/e3sconf_icvaes2020_01031.pdf |
_version_ | 1818645234133762048 |
---|---|
author | Airin Claude M. Hana Amelia Sarmin Sarmin Astuti Pudji |
author_facet | Airin Claude M. Hana Amelia Sarmin Sarmin Astuti Pudji |
author_sort | Airin Claude M. |
collection | DOAJ |
description | Progesterone (P4) is a dominant hormone during pregnancy. In the later stage of pregnancy, the stress hormone particularly cortisol (C) may increase for initiating the parturition process as a consequence of fetal stress. This study was a preliminary study to compare the concentration of P4 and C in feces of Etawah Crossbreed Goat during their last stage of pregnancy and post partus. This study used 5 pregnant Etawah Crossbreed Goats (t 20th weeks) of pregnancy. Fecal samples were collected in the 20th week of pregnancy to 2 weeks of postpartum. All fecal samples were then dried using a freeze dryer (Labfreez FD10-MR) for 7 days at -80°C. Afterward, dried feces were pulverized and extracted by using 3ml of methanol 80%. The fecal extract was then analyzed the P4 and C concentrations using the enzyme-linked immunosorbent assay method. Concentrations of P4 and C metabolites in the last stage of pregnancy were 5,506.18 3,396.72 ng/g dry feces and 136,625.83 42,479.22 ng/g feces, respectively. Concentrations of P4 and C metabolites in the 2 weeks postpartum decreased at 669.38 P 643.9 ng/g feces and 110,295 / 14,378, 8 ng/g feces, respectively. It canbe concluded that there was a difference in the fecal progesterone and cortisol concentrations between the last phase of pregnancy and the postpartum phase. |
first_indexed | 2024-12-17T00:27:30Z |
format | Article |
id | doaj.art-d694a88b49a449cdbb2a665c8c338c48 |
institution | Directory Open Access Journal |
issn | 2267-1242 |
language | English |
last_indexed | 2024-12-17T00:27:30Z |
publishDate | 2020-01-01 |
publisher | EDP Sciences |
record_format | Article |
series | E3S Web of Conferences |
spelling | doaj.art-d694a88b49a449cdbb2a665c8c338c482022-12-21T22:10:26ZengEDP SciencesE3S Web of Conferences2267-12422020-01-011510103110.1051/e3sconf/202015101031e3sconf_icvaes2020_01031Fecal Cortisol and Progesterone Concentrations in Post Partus of Etawah Crossbreed GoatAirin Claude M.0Hana Amelia1Sarmin Sarmin2Astuti Pudji3Department of Physiology, Faculty of Veterinary Medicine, Universitas Gadjah MadaDepartment of Physiology, Faculty of Veterinary Medicine, Universitas Gadjah MadaDepartment of Physiology, Faculty of Veterinary Medicine, Universitas Gadjah MadaDepartment of Physiology, Faculty of Veterinary Medicine, Universitas Gadjah MadaProgesterone (P4) is a dominant hormone during pregnancy. In the later stage of pregnancy, the stress hormone particularly cortisol (C) may increase for initiating the parturition process as a consequence of fetal stress. This study was a preliminary study to compare the concentration of P4 and C in feces of Etawah Crossbreed Goat during their last stage of pregnancy and post partus. This study used 5 pregnant Etawah Crossbreed Goats (t 20th weeks) of pregnancy. Fecal samples were collected in the 20th week of pregnancy to 2 weeks of postpartum. All fecal samples were then dried using a freeze dryer (Labfreez FD10-MR) for 7 days at -80°C. Afterward, dried feces were pulverized and extracted by using 3ml of methanol 80%. The fecal extract was then analyzed the P4 and C concentrations using the enzyme-linked immunosorbent assay method. Concentrations of P4 and C metabolites in the last stage of pregnancy were 5,506.18 3,396.72 ng/g dry feces and 136,625.83 42,479.22 ng/g feces, respectively. Concentrations of P4 and C metabolites in the 2 weeks postpartum decreased at 669.38 P 643.9 ng/g feces and 110,295 / 14,378, 8 ng/g feces, respectively. It canbe concluded that there was a difference in the fecal progesterone and cortisol concentrations between the last phase of pregnancy and the postpartum phase.https://www.e3s-conferences.org/articles/e3sconf/pdf/2020/11/e3sconf_icvaes2020_01031.pdfcrossbreedfecesmetabolite hormonecortisolprogesterone |
spellingShingle | Airin Claude M. Hana Amelia Sarmin Sarmin Astuti Pudji Fecal Cortisol and Progesterone Concentrations in Post Partus of Etawah Crossbreed Goat E3S Web of Conferences crossbreed feces metabolite hormone cortisol progesterone |
title | Fecal Cortisol and Progesterone Concentrations in Post Partus of Etawah Crossbreed Goat |
title_full | Fecal Cortisol and Progesterone Concentrations in Post Partus of Etawah Crossbreed Goat |
title_fullStr | Fecal Cortisol and Progesterone Concentrations in Post Partus of Etawah Crossbreed Goat |
title_full_unstemmed | Fecal Cortisol and Progesterone Concentrations in Post Partus of Etawah Crossbreed Goat |
title_short | Fecal Cortisol and Progesterone Concentrations in Post Partus of Etawah Crossbreed Goat |
title_sort | fecal cortisol and progesterone concentrations in post partus of etawah crossbreed goat |
topic | crossbreed feces metabolite hormone cortisol progesterone |
url | https://www.e3s-conferences.org/articles/e3sconf/pdf/2020/11/e3sconf_icvaes2020_01031.pdf |
work_keys_str_mv | AT airinclaudem fecalcortisolandprogesteroneconcentrationsinpostpartusofetawahcrossbreedgoat AT hanaamelia fecalcortisolandprogesteroneconcentrationsinpostpartusofetawahcrossbreedgoat AT sarminsarmin fecalcortisolandprogesteroneconcentrationsinpostpartusofetawahcrossbreedgoat AT astutipudji fecalcortisolandprogesteroneconcentrationsinpostpartusofetawahcrossbreedgoat |