Characterization and genetic diversity analysis of cotton cultivars using microsatellites
Genetic diversity and the relationship between varieties are of great importance for cotton breeding. Our work was designed to estimate the informativeness of the cotton (Gossypium hirsutum L.) simple sequence repeat (SSR) microsatellite locus and to estimate the genetic distance between 53 cotton c...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2006-01-01
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| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200021 |
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| author | Cândida H.C. de Magalhães Bertini Ivan Schuster Tocio Sediyama Everaldo Gonçalves de Barros Maurílio Alves Moreira |
| author_facet | Cândida H.C. de Magalhães Bertini Ivan Schuster Tocio Sediyama Everaldo Gonçalves de Barros Maurílio Alves Moreira |
| author_sort | Cândida H.C. de Magalhães Bertini |
| collection | DOAJ |
| description | Genetic diversity and the relationship between varieties are of great importance for cotton breeding. Our work was designed to estimate the informativeness of the cotton (Gossypium hirsutum L.) simple sequence repeat (SSR) microsatellite locus and to estimate the genetic distance between 53 cotton cultivars as well as to select a set of SSR primers able to differentiate between the 53 cotton cultivars studied. After extracting DNA from the 53 cultivars and characterized it using 31 pairs of SSR primers we obtained a total of 66 alleles with an average of 2.13 alleles per SSR locus and values of polymorphism information content (PIC) varying from 0.18 to 0.62, the dissimilarity coefficient varying from zero to 0.41. Statistical analysis using the unweighted pair-group method using arithmetic average (UPGMA) revealed seven subgroups which were consistent with the genealogical information available for some of the cultivars. The SSR genetic profile obtained for each of the cultivars made it possible to discriminate 52 of the 53 cultivars. This study of the genetic diversity of cotton cultivars with SSR markers support the need to introduce new alleles into the gene pool of the breeding cultivars. |
| first_indexed | 2024-12-21T21:15:24Z |
| format | Article |
| id | doaj.art-d69b50b815ee46cba6a9c4206f599291 |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-21T21:15:24Z |
| publishDate | 2006-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-d69b50b815ee46cba6a9c4206f5992912022-12-21T18:50:01ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852006-01-0129232132910.1590/S1415-47572006000200021Characterization and genetic diversity analysis of cotton cultivars using microsatellitesCândida H.C. de Magalhães BertiniIvan SchusterTocio SediyamaEveraldo Gonçalves de BarrosMaurílio Alves MoreiraGenetic diversity and the relationship between varieties are of great importance for cotton breeding. Our work was designed to estimate the informativeness of the cotton (Gossypium hirsutum L.) simple sequence repeat (SSR) microsatellite locus and to estimate the genetic distance between 53 cotton cultivars as well as to select a set of SSR primers able to differentiate between the 53 cotton cultivars studied. After extracting DNA from the 53 cultivars and characterized it using 31 pairs of SSR primers we obtained a total of 66 alleles with an average of 2.13 alleles per SSR locus and values of polymorphism information content (PIC) varying from 0.18 to 0.62, the dissimilarity coefficient varying from zero to 0.41. Statistical analysis using the unweighted pair-group method using arithmetic average (UPGMA) revealed seven subgroups which were consistent with the genealogical information available for some of the cultivars. The SSR genetic profile obtained for each of the cultivars made it possible to discriminate 52 of the 53 cultivars. This study of the genetic diversity of cotton cultivars with SSR markers support the need to introduce new alleles into the gene pool of the breeding cultivars.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200021fingerprintingGossypium hirsutum L.genealogymolecular markers |
| spellingShingle | Cândida H.C. de Magalhães Bertini Ivan Schuster Tocio Sediyama Everaldo Gonçalves de Barros Maurílio Alves Moreira Characterization and genetic diversity analysis of cotton cultivars using microsatellites Genetics and Molecular Biology fingerprinting Gossypium hirsutum L. genealogy molecular markers |
| title | Characterization and genetic diversity analysis of cotton cultivars using microsatellites |
| title_full | Characterization and genetic diversity analysis of cotton cultivars using microsatellites |
| title_fullStr | Characterization and genetic diversity analysis of cotton cultivars using microsatellites |
| title_full_unstemmed | Characterization and genetic diversity analysis of cotton cultivars using microsatellites |
| title_short | Characterization and genetic diversity analysis of cotton cultivars using microsatellites |
| title_sort | characterization and genetic diversity analysis of cotton cultivars using microsatellites |
| topic | fingerprinting Gossypium hirsutum L. genealogy molecular markers |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200021 |
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