Improving the Therapeutic Performance of Glycyrrhiza Glabra Hydroalcoholic Extract Using Liposomal Nano-carriers and Their Characterization
Background: The use of plants as therapeutic drugs has long been common among human beings. The Glycyrrhiza glabra is one of the medicinal plants with many therapeutic properties. However, using this herb in traditional methods faces some challenges. The use of pharmaceutical nano-carriers such as l...
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| Format: | Article |
| Language: | English |
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Hormozgan University of Medical Sciences
2022-04-01
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| Series: | Disease and Diagnosis |
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| Online Access: | https://ddj.hums.ac.ir/PDF/ddj-11-39.pdf |
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| author | Milad Akhlaghi Lida Eftekharivash Mohammad Taebpour Saeid Afereydoon Marziyeh Ebrahimpour Maryam Zarezadeh Mehrizi Mahboobeh Zarezadeh Mehrizi Bibi fatemeh Haghirosadat |
| author_facet | Milad Akhlaghi Lida Eftekharivash Mohammad Taebpour Saeid Afereydoon Marziyeh Ebrahimpour Maryam Zarezadeh Mehrizi Mahboobeh Zarezadeh Mehrizi Bibi fatemeh Haghirosadat |
| author_sort | Milad Akhlaghi |
| collection | DOAJ |
| description | Background: The use of plants as therapeutic drugs has long been common among human beings. The Glycyrrhiza glabra is one of the medicinal plants with many therapeutic properties. However, using this herb in traditional methods faces some challenges. The use of pharmaceutical nano-carriers such as liposomes is one of the new strategies to overcome these challenges. In this regard, the current study aimed to synthesize and characterize liposomal nano-carriers containing the G. glabra hydroalcoholic extract to improve its therapeutic effects. Materials and Methods: After the extraction of the G. glabra root by the Soxhlet method, nano-liposomes containing G. glabra extracts were synthesized by the thin-film preparation method. Then, the encapsulation efficiency (EE) rate and drug release pattern of nanoliposome were examined using the spectrophotometry method. Next, physicochemical properties such as size, zeta potential, morphology, and non-interaction of the nano-system with the extract were investigated by dynamic-light-scattering (DLS), atomic force microscope (AFM), and Fourier transform infrared spectroscopy (FTIR) methods, and finally, the toxicity of the nano-system on human foreskin fibroblast cells was assessed using the MTT method. Results: Nano-liposomes containing licorice extracts with the EE of 2.3±75.32% were from the type of slow release and controlled release, having a size of 111.4±1.2 nm, a surface charge of -53.6±6.3, and a dispersion index of 0.210±0.13, and they had no interaction with the loaded extract. The results of the MTT test also demonstrated that the synthesized nano-liposomes were non-toxic on normal cells. Conclusion: Overall, the findings proved that synthesized nano-liposomes with proper physicochemical properties can be a suitable carrier for the G. glabra extract and thus cause stability and improve the therapeutic effects of this herbal extract as a medicinal plant. |
| first_indexed | 2024-04-12T16:15:01Z |
| format | Article |
| id | doaj.art-d69d16a762c446c3bd1cf4a5c9d24ab3 |
| institution | Directory Open Access Journal |
| issn | 2717-3232 |
| language | English |
| last_indexed | 2024-04-12T16:15:01Z |
| publishDate | 2022-04-01 |
| publisher | Hormozgan University of Medical Sciences |
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| series | Disease and Diagnosis |
| spelling | doaj.art-d69d16a762c446c3bd1cf4a5c9d24ab32022-12-22T03:25:46ZengHormozgan University of Medical SciencesDisease and Diagnosis2717-32322022-04-01112394810.34172/ddj.2022.09ddj-302Improving the Therapeutic Performance of Glycyrrhiza Glabra Hydroalcoholic Extract Using Liposomal Nano-carriers and Their CharacterizationMilad Akhlaghi0Lida Eftekharivash1Mohammad Taebpour2Saeid Afereydoon3Marziyeh Ebrahimpour4Maryam Zarezadeh Mehrizi5Mahboobeh Zarezadeh Mehrizi6Bibi fatemeh Haghirosadat7Department of Clinical Biochemistry, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.Assistant Professor of Microbiology, Maragheh Branch, Islamic Azad University. Maragheh, Iran.Nano-Biotech Foresight Company Biotechnology Campus, Yazd Stem Cells and Regenerative Medicine Institute, Yazd, Iran.Department of Medical Physics, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.Nano-Biotech Foresight Company Biotechnology Campus, Yazd Stem Cells and Regenerative Medicine Institute, Yazd, Iran.Faculty of Dentistry, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.Faculty of Dentistry, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.Nano-Biotech Foresight Company Biotechnology Campus, Yazd Stem Cells and Regenerative Medicine Institute, Yazd, Iran.Background: The use of plants as therapeutic drugs has long been common among human beings. The Glycyrrhiza glabra is one of the medicinal plants with many therapeutic properties. However, using this herb in traditional methods faces some challenges. The use of pharmaceutical nano-carriers such as liposomes is one of the new strategies to overcome these challenges. In this regard, the current study aimed to synthesize and characterize liposomal nano-carriers containing the G. glabra hydroalcoholic extract to improve its therapeutic effects. Materials and Methods: After the extraction of the G. glabra root by the Soxhlet method, nano-liposomes containing G. glabra extracts were synthesized by the thin-film preparation method. Then, the encapsulation efficiency (EE) rate and drug release pattern of nanoliposome were examined using the spectrophotometry method. Next, physicochemical properties such as size, zeta potential, morphology, and non-interaction of the nano-system with the extract were investigated by dynamic-light-scattering (DLS), atomic force microscope (AFM), and Fourier transform infrared spectroscopy (FTIR) methods, and finally, the toxicity of the nano-system on human foreskin fibroblast cells was assessed using the MTT method. Results: Nano-liposomes containing licorice extracts with the EE of 2.3±75.32% were from the type of slow release and controlled release, having a size of 111.4±1.2 nm, a surface charge of -53.6±6.3, and a dispersion index of 0.210±0.13, and they had no interaction with the loaded extract. The results of the MTT test also demonstrated that the synthesized nano-liposomes were non-toxic on normal cells. Conclusion: Overall, the findings proved that synthesized nano-liposomes with proper physicochemical properties can be a suitable carrier for the G. glabra extract and thus cause stability and improve the therapeutic effects of this herbal extract as a medicinal plant.https://ddj.hums.ac.ir/PDF/ddj-11-39.pdfliposomeglycrrhiza glabraencapsulation efficiencymtt assayslow-release |
| spellingShingle | Milad Akhlaghi Lida Eftekharivash Mohammad Taebpour Saeid Afereydoon Marziyeh Ebrahimpour Maryam Zarezadeh Mehrizi Mahboobeh Zarezadeh Mehrizi Bibi fatemeh Haghirosadat Improving the Therapeutic Performance of Glycyrrhiza Glabra Hydroalcoholic Extract Using Liposomal Nano-carriers and Their Characterization Disease and Diagnosis liposome glycrrhiza glabra encapsulation efficiency mtt assay slow-release |
| title | Improving the Therapeutic Performance of Glycyrrhiza Glabra Hydroalcoholic Extract Using Liposomal Nano-carriers and Their Characterization |
| title_full | Improving the Therapeutic Performance of Glycyrrhiza Glabra Hydroalcoholic Extract Using Liposomal Nano-carriers and Their Characterization |
| title_fullStr | Improving the Therapeutic Performance of Glycyrrhiza Glabra Hydroalcoholic Extract Using Liposomal Nano-carriers and Their Characterization |
| title_full_unstemmed | Improving the Therapeutic Performance of Glycyrrhiza Glabra Hydroalcoholic Extract Using Liposomal Nano-carriers and Their Characterization |
| title_short | Improving the Therapeutic Performance of Glycyrrhiza Glabra Hydroalcoholic Extract Using Liposomal Nano-carriers and Their Characterization |
| title_sort | improving the therapeutic performance of glycyrrhiza glabra hydroalcoholic extract using liposomal nano carriers and their characterization |
| topic | liposome glycrrhiza glabra encapsulation efficiency mtt assay slow-release |
| url | https://ddj.hums.ac.ir/PDF/ddj-11-39.pdf |
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