Mongolian Rotifers on Micr oscope Slides: Instructions to Permanent Specimen Mounts from Expedition Material

We here describe a method for permanently mounting specimens on microscope slides, as we applied it in the newly established rotifer collection in Ulaanbaatar, Mongolia. The liquid photopolymer NOA 61 was used as a primary sealant for pure glycerine mounts....

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Main Authors: Christian D. Jersabek, Erdene Bolortsetseg, Howard L. Taylor
Format: Article
Language:English
Published: National University of Mongolia 2010-06-01
Series:Mongolian Journal of Biological Sciences
Subjects:
Online Access:http://mjbs.num.edu.mn/uploads/files/MJBS%20Volume%208%20Number%201%20June%202010/pdf/mjbs008-01-06.pdf
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author Christian D. Jersabek
Erdene Bolortsetseg
Howard L. Taylor
author_facet Christian D. Jersabek
Erdene Bolortsetseg
Howard L. Taylor
author_sort Christian D. Jersabek
collection DOAJ
description We here describe a method for permanently mounting specimens on microscope slides, as we applied it in the newly established rotifer collection in Ulaanbaatar, Mongolia. The liquid photopolymer NOA 61 was used as a primary sealant for pure glycerine mounts. We furthermore outline simple methods of rotifer narcotization and fi xation in the fi eld that yield, for the majority of species, adequately preserved specimen material for further preparation and identifi cation purposes.
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spelling doaj.art-d6a18e719c1948fb8749ecbe7b7044552022-12-21T19:26:14ZengNational University of MongoliaMongolian Journal of Biological Sciences1684-39082225-49942010-06-0181515710.22353/mjbs.2010.08.06Mongolian Rotifers on Micr oscope Slides: Instructions to Permanent Specimen Mounts from Expedition MaterialChristian D. Jersabek0Erdene Bolortsetseg1Howard L. Taylor2Department of Organismal Biology , University of Salzburg, A–5020 Salzburg, Austria, and Academy of Natural Sciences, Center for Systematic Biology & Evolution, Philadelphia, USAInstitute of Meteorology and Hydrology, Laboratory of Hydrobiology, Ulaanbaatar, MongoliaTaylor Laboratory, Sarasota, USAWe here describe a method for permanently mounting specimens on microscope slides, as we applied it in the newly established rotifer collection in Ulaanbaatar, Mongolia. The liquid photopolymer NOA 61 was used as a primary sealant for pure glycerine mounts. We furthermore outline simple methods of rotifer narcotization and fi xation in the fi eld that yield, for the majority of species, adequately preserved specimen material for further preparation and identifi cation purposes.http://mjbs.num.edu.mn/uploads/files/MJBS%20Volume%208%20Number%201%20June%202010/pdf/mjbs008-01-06.pdfRotiferanarcosisbulk fi xationglycerine mountmicroscope slide preparationbupivacaine
spellingShingle Christian D. Jersabek
Erdene Bolortsetseg
Howard L. Taylor
Mongolian Rotifers on Micr oscope Slides: Instructions to Permanent Specimen Mounts from Expedition Material
Mongolian Journal of Biological Sciences
Rotifera
narcosis
bulk fi xation
glycerine mount
microscope slide preparation
bupivacaine
title Mongolian Rotifers on Micr oscope Slides: Instructions to Permanent Specimen Mounts from Expedition Material
title_full Mongolian Rotifers on Micr oscope Slides: Instructions to Permanent Specimen Mounts from Expedition Material
title_fullStr Mongolian Rotifers on Micr oscope Slides: Instructions to Permanent Specimen Mounts from Expedition Material
title_full_unstemmed Mongolian Rotifers on Micr oscope Slides: Instructions to Permanent Specimen Mounts from Expedition Material
title_short Mongolian Rotifers on Micr oscope Slides: Instructions to Permanent Specimen Mounts from Expedition Material
title_sort mongolian rotifers on micr oscope slides instructions to permanent specimen mounts from expedition material
topic Rotifera
narcosis
bulk fi xation
glycerine mount
microscope slide preparation
bupivacaine
url http://mjbs.num.edu.mn/uploads/files/MJBS%20Volume%208%20Number%201%20June%202010/pdf/mjbs008-01-06.pdf
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AT howardltaylor mongolianrotifersonmicroscopeslidesinstructionstopermanentspecimenmountsfromexpeditionmaterial