Ekstrak Etanol Daun Kacang Panjang (Vigna unguiculata (L.) Walp): Kajian Morfometri Insula Pankreatika Model Tikus Diabetes
Diabetes is characterized by an increasing of blood levels and progressive changes in the structure of pancreatic islet of pancreas. Natural ingredients that contain antioxidants such as some cowpea leaves can be an alternative treatment for diabetes. This study aims to examine the effect of ethanol...
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| Format: | Article |
| Language: | English |
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Universitas Tadulako
2019-10-01
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| Series: | Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) |
| Subjects: | |
| Online Access: | http://jurnal.untad.ac.id/jurnal/index.php/Galenika/article/view/13934 |
| _version_ | 1811225015651139584 |
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| author | Puput Octaviani Solehah Fikhta Agnesya Tarusu Joni Tandi Niluh Puspita Dewi Gabriella Bamba Ratih Lintin Yuli Fitriana David Pakaya |
| author_facet | Puput Octaviani Solehah Fikhta Agnesya Tarusu Joni Tandi Niluh Puspita Dewi Gabriella Bamba Ratih Lintin Yuli Fitriana David Pakaya |
| author_sort | Puput Octaviani Solehah |
| collection | DOAJ |
| description | Diabetes is characterized by an increasing of blood levels and progressive changes in the structure of pancreatic islet of pancreas. Natural ingredients that contain antioxidants such as some cowpea leaves can be an alternative treatment for diabetes. This study aims to examine the effect of ethanol extract of some cowepea leaves (Vigna unguiculata (L.) Walp) on morphometry (perimeter and diameter) of pancreatic islet of diabetic rat models. The type of research is quasi-experimental with posttest group design. Samples were 30 male wistar rats, age 8 weeks, BW 250-300 grams, that induced by single dose of STZ 40 mg/kg BW. Rats were divided into 5 groups. Group A: normal control, Group B: negative control, Group C: positive control, Group D: DM rats with 100mg/Kg BW extract therapy, Group E: DM rats with 200mg/Kg BW extract therapy, Group F: DM rats with extract therapy 300mg/Kg BW. Blood sugar levels were measured on days 7, 14 and 21. Pancreatic tissue was prepared with 5μm thickness and stained with Hematoxylin Eosin. The sample was observed under 400x magnification microscope. The observation uses Image J software to measure the perimeter and diameter of the pancreatic insula. Data were analyzed by non-parametric Kruskal Wallis and post hoct Mann Whitney statistical tests. Blood glucose level was obtained on 21st day with the highest mean in group B (246.8±156.3) and lowest in group A (67.2 ±7.85), perimeter of pancreatic islet was significantly different (p=0.026), the diameter is significantly different (p =0.046). Ethanol extract of some cowpea (Vigna unguiculata (L.) Walp) leaves with dose of 300mg/kgBB helps to restore the blood and morphometry of pancreatic islet model of diabetic rats. |
| first_indexed | 2024-04-12T08:58:43Z |
| format | Article |
| id | doaj.art-d6a1e28e000c412ab41bd49cd284b258 |
| institution | Directory Open Access Journal |
| issn | 2442-7284 2442-8744 |
| language | English |
| last_indexed | 2024-04-12T08:58:43Z |
| publishDate | 2019-10-01 |
| publisher | Universitas Tadulako |
| record_format | Article |
| series | Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) |
| spelling | doaj.art-d6a1e28e000c412ab41bd49cd284b2582022-12-22T03:39:19ZengUniversitas TadulakoJurnal Farmasi Galenika (Galenika Journal of Pharmacy)2442-72842442-87442019-10-015218318810.22487/j24428744.2019.v5.i2.1393410708Ekstrak Etanol Daun Kacang Panjang (Vigna unguiculata (L.) Walp): Kajian Morfometri Insula Pankreatika Model Tikus DiabetesPuput Octaviani Solehah0Fikhta Agnesya Tarusu1Joni Tandi2Niluh Puspita Dewi3Gabriella Bamba Ratih Lintin4Yuli Fitriana5David Pakaya6Program Studi Ilmu Farmasi, Sekolah Tinggi Ilmu Farmasi Pelita Mas, PaluProgram Studi Ilmu Farmasi, Sekolah Tinggi Ilmu Farmasi Pelita Mas, PaluProgram Studi Ilmu Farmasi, Sekolah Tinggi Ilmu Farmasi Pelita Mas, PaluProgram Studi Ilmu Farmasi, Sekolah Tinggi Ilmu Farmasi Pelita Mas, PaluDepartemen Anatomi, Fakultas Kedokteran, Universitas Tadulako, PaluDepartemen Histologi, Fakultas Kedokteran, Universitas Tadulako, PaluDepartemen Histologi, Fakultas Kedokteran, Universitas Tadulako, PaluDiabetes is characterized by an increasing of blood levels and progressive changes in the structure of pancreatic islet of pancreas. Natural ingredients that contain antioxidants such as some cowpea leaves can be an alternative treatment for diabetes. This study aims to examine the effect of ethanol extract of some cowepea leaves (Vigna unguiculata (L.) Walp) on morphometry (perimeter and diameter) of pancreatic islet of diabetic rat models. The type of research is quasi-experimental with posttest group design. Samples were 30 male wistar rats, age 8 weeks, BW 250-300 grams, that induced by single dose of STZ 40 mg/kg BW. Rats were divided into 5 groups. Group A: normal control, Group B: negative control, Group C: positive control, Group D: DM rats with 100mg/Kg BW extract therapy, Group E: DM rats with 200mg/Kg BW extract therapy, Group F: DM rats with extract therapy 300mg/Kg BW. Blood sugar levels were measured on days 7, 14 and 21. Pancreatic tissue was prepared with 5μm thickness and stained with Hematoxylin Eosin. The sample was observed under 400x magnification microscope. The observation uses Image J software to measure the perimeter and diameter of the pancreatic insula. Data were analyzed by non-parametric Kruskal Wallis and post hoct Mann Whitney statistical tests. Blood glucose level was obtained on 21st day with the highest mean in group B (246.8±156.3) and lowest in group A (67.2 ±7.85), perimeter of pancreatic islet was significantly different (p=0.026), the diameter is significantly different (p =0.046). Ethanol extract of some cowpea (Vigna unguiculata (L.) Walp) leaves with dose of 300mg/kgBB helps to restore the blood and morphometry of pancreatic islet model of diabetic rats.http://jurnal.untad.ac.id/jurnal/index.php/Galenika/article/view/13934vigna unguiculata (l.) walp, diabetes, morphometry, pancreatic islet |
| spellingShingle | Puput Octaviani Solehah Fikhta Agnesya Tarusu Joni Tandi Niluh Puspita Dewi Gabriella Bamba Ratih Lintin Yuli Fitriana David Pakaya Ekstrak Etanol Daun Kacang Panjang (Vigna unguiculata (L.) Walp): Kajian Morfometri Insula Pankreatika Model Tikus Diabetes Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) vigna unguiculata (l.) walp, diabetes, morphometry, pancreatic islet |
| title | Ekstrak Etanol Daun Kacang Panjang (Vigna unguiculata (L.) Walp): Kajian Morfometri Insula Pankreatika Model Tikus Diabetes |
| title_full | Ekstrak Etanol Daun Kacang Panjang (Vigna unguiculata (L.) Walp): Kajian Morfometri Insula Pankreatika Model Tikus Diabetes |
| title_fullStr | Ekstrak Etanol Daun Kacang Panjang (Vigna unguiculata (L.) Walp): Kajian Morfometri Insula Pankreatika Model Tikus Diabetes |
| title_full_unstemmed | Ekstrak Etanol Daun Kacang Panjang (Vigna unguiculata (L.) Walp): Kajian Morfometri Insula Pankreatika Model Tikus Diabetes |
| title_short | Ekstrak Etanol Daun Kacang Panjang (Vigna unguiculata (L.) Walp): Kajian Morfometri Insula Pankreatika Model Tikus Diabetes |
| title_sort | ekstrak etanol daun kacang panjang vigna unguiculata l walp kajian morfometri insula pankreatika model tikus diabetes |
| topic | vigna unguiculata (l.) walp, diabetes, morphometry, pancreatic islet |
| url | http://jurnal.untad.ac.id/jurnal/index.php/Galenika/article/view/13934 |
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