Histological and molecular difference in albumen quality between post-adolescent hens and aged hens
ABSTRACT: The decline in albumen quality resulting from aging hens poses a threat to the financial benefits of the egg industry. Exploring the underlying mechanisms from the perspective of cell molecules of albumen formation is significant for the efficient regulation of albumen quality. Two individ...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-06-01
|
| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124001974 |
| _version_ | 1797226722092908544 |
|---|---|
| author | Xin-yu Chang Obianwuna Uchechukwu Edna Jing Wang Hai-jun Zhang Jian-min Zhou Kai Qiu Shu-geng Wu |
| author_facet | Xin-yu Chang Obianwuna Uchechukwu Edna Jing Wang Hai-jun Zhang Jian-min Zhou Kai Qiu Shu-geng Wu |
| author_sort | Xin-yu Chang |
| collection | DOAJ |
| description | ABSTRACT: The decline in albumen quality resulting from aging hens poses a threat to the financial benefits of the egg industry. Exploring the underlying mechanisms from the perspective of cell molecules of albumen formation is significant for the efficient regulation of albumen quality. Two individual groups of Hy-Line Brown layers with ages of 40 (W40) and 100 (W100) wk old were used in the present study. Each group contained over 2,000 birds. This study assessed the egg quality, biochemical indicators and physiological status of hens between W40 and W100. Subsequently, a quantitative proteomic analysis was conducted to identify differences in protein abundance in magnum tissues between W40 and W100. In the W40 group, significant increases (P < 0.05) were notable for albumen quality (thick albumen solid content, albumen height, Haugh unit), serum indices (calcium, estrogen, and progesterone levels), magnum histomorphology (myosin light-chain kinase content, secretory capacity, mucosal fold, goblet cell count and proportion) as well as the total antioxidant capacity of the liver. However, the luminal diameter of the magnum, albumen gel properties and random coil of the albumen were increased (P < 0.05) in the W100 group. The activity of glutathione, superoxidase dismutase, and malondialdehyde in the liver, magnum, and serum did not vary (P > 0.05) among the groups. Proteomic analysis revealed the identification of 118 differentially expressed proteins between the groups, which comprised proteins associated with protein secretion, DNA damage and repair, cell proliferation, growth, antioxidants, and apoptosis. Furthermore, Kyoto Encyclopedia of Genes pathway analysis revealed that BRCA2 and FBN1 were significantly downregulated in Fanconi anemia (FA) and TGF-β signaling pathways in W100, validated through quantitative real-time PCR (qRT-PCR). In conclusion, significant age-related variations in albumen quality, and magnum morphology are regulated by proteins involved in antioxidant capacity. |
| first_indexed | 2024-04-24T14:29:25Z |
| format | Article |
| id | doaj.art-d6a5873a590040debdf73923905d97fe |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-04-24T14:29:25Z |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-d6a5873a590040debdf73923905d97fe2024-04-03T04:25:55ZengElsevierPoultry Science0032-57912024-06-011036103618Histological and molecular difference in albumen quality between post-adolescent hens and aged hensXin-yu Chang0Obianwuna Uchechukwu Edna1Jing Wang2Hai-jun Zhang3Jian-min Zhou4Kai Qiu5Shu-geng Wu6Key Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, Laboratory of Quality & Safety Risk Assessment for Products on Feed-origin Risk Factor, Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaKey Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, Laboratory of Quality & Safety Risk Assessment for Products on Feed-origin Risk Factor, Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaKey Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, Laboratory of Quality & Safety Risk Assessment for Products on Feed-origin Risk Factor, Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaKey Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, Laboratory of Quality & Safety Risk Assessment for Products on Feed-origin Risk Factor, Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaKey Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, Laboratory of Quality & Safety Risk Assessment for Products on Feed-origin Risk Factor, Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaCorresponding author:; Key Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, Laboratory of Quality & Safety Risk Assessment for Products on Feed-origin Risk Factor, Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaKey Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, Laboratory of Quality & Safety Risk Assessment for Products on Feed-origin Risk Factor, Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaABSTRACT: The decline in albumen quality resulting from aging hens poses a threat to the financial benefits of the egg industry. Exploring the underlying mechanisms from the perspective of cell molecules of albumen formation is significant for the efficient regulation of albumen quality. Two individual groups of Hy-Line Brown layers with ages of 40 (W40) and 100 (W100) wk old were used in the present study. Each group contained over 2,000 birds. This study assessed the egg quality, biochemical indicators and physiological status of hens between W40 and W100. Subsequently, a quantitative proteomic analysis was conducted to identify differences in protein abundance in magnum tissues between W40 and W100. In the W40 group, significant increases (P < 0.05) were notable for albumen quality (thick albumen solid content, albumen height, Haugh unit), serum indices (calcium, estrogen, and progesterone levels), magnum histomorphology (myosin light-chain kinase content, secretory capacity, mucosal fold, goblet cell count and proportion) as well as the total antioxidant capacity of the liver. However, the luminal diameter of the magnum, albumen gel properties and random coil of the albumen were increased (P < 0.05) in the W100 group. The activity of glutathione, superoxidase dismutase, and malondialdehyde in the liver, magnum, and serum did not vary (P > 0.05) among the groups. Proteomic analysis revealed the identification of 118 differentially expressed proteins between the groups, which comprised proteins associated with protein secretion, DNA damage and repair, cell proliferation, growth, antioxidants, and apoptosis. Furthermore, Kyoto Encyclopedia of Genes pathway analysis revealed that BRCA2 and FBN1 were significantly downregulated in Fanconi anemia (FA) and TGF-β signaling pathways in W100, validated through quantitative real-time PCR (qRT-PCR). In conclusion, significant age-related variations in albumen quality, and magnum morphology are regulated by proteins involved in antioxidant capacity.http://www.sciencedirect.com/science/article/pii/S0032579124001974laying henalbumen qualitymagnum morphologyproteomicsantioxidant capacity |
| spellingShingle | Xin-yu Chang Obianwuna Uchechukwu Edna Jing Wang Hai-jun Zhang Jian-min Zhou Kai Qiu Shu-geng Wu Histological and molecular difference in albumen quality between post-adolescent hens and aged hens Poultry Science laying hen albumen quality magnum morphology proteomics antioxidant capacity |
| title | Histological and molecular difference in albumen quality between post-adolescent hens and aged hens |
| title_full | Histological and molecular difference in albumen quality between post-adolescent hens and aged hens |
| title_fullStr | Histological and molecular difference in albumen quality between post-adolescent hens and aged hens |
| title_full_unstemmed | Histological and molecular difference in albumen quality between post-adolescent hens and aged hens |
| title_short | Histological and molecular difference in albumen quality between post-adolescent hens and aged hens |
| title_sort | histological and molecular difference in albumen quality between post adolescent hens and aged hens |
| topic | laying hen albumen quality magnum morphology proteomics antioxidant capacity |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124001974 |
| work_keys_str_mv | AT xinyuchang histologicalandmoleculardifferenceinalbumenqualitybetweenpostadolescenthensandagedhens AT obianwunauchechukwuedna histologicalandmoleculardifferenceinalbumenqualitybetweenpostadolescenthensandagedhens AT jingwang histologicalandmoleculardifferenceinalbumenqualitybetweenpostadolescenthensandagedhens AT haijunzhang histologicalandmoleculardifferenceinalbumenqualitybetweenpostadolescenthensandagedhens AT jianminzhou histologicalandmoleculardifferenceinalbumenqualitybetweenpostadolescenthensandagedhens AT kaiqiu histologicalandmoleculardifferenceinalbumenqualitybetweenpostadolescenthensandagedhens AT shugengwu histologicalandmoleculardifferenceinalbumenqualitybetweenpostadolescenthensandagedhens |