Characteristics of Phenotypic and Genetic Properties of <i>Francisella tularensis</i> 15 NIIEG Vaccine Strain with an Extended Storage Period
Investigated have been cultural-morphological, biochemical and genetic properties of lyophilized cultures of F. tularensis 15 NIIEG vaccine strain, accumulated within 60-years term and deposited at the State Collection of Pathogenic Microorganisms of Scientific Center on Expertise of Medical Applica...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | Russian |
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Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
2015-12-01
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| Series: | Проблемы особо опасных инфекций |
| Subjects: | |
| Online Access: | https://journal.microbe.ru/jour/article/view/273 |
| _version_ | 1826922406272303104 |
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| author | E. A. Solov’Ev L. V. Sayapina N. A. Osina D. S. Davydov V. P. Bondarev |
| author_facet | E. A. Solov’Ev L. V. Sayapina N. A. Osina D. S. Davydov V. P. Bondarev |
| author_sort | E. A. Solov’Ev |
| collection | DOAJ |
| description | Investigated have been cultural-morphological, biochemical and genetic properties of lyophilized cultures of F. tularensis 15 NIIEG vaccine strain, accumulated within 60-years term and deposited at the State Collection of Pathogenic Microorganisms of Scientific Center on Expertise of Medical Application Products. The studies undertaken have demonstrated that storing of the strains in such a form at low temperatures, does not prevent changes of their genetic and phenotypic properties to the full extent. It is established that F. tularensis 15 NIIEG strain lyophilized in 1953, 1966, 1969, 2003 and 2012 maintains its immunogenic properties when cultivated on nutrient media Ft-agar with or without addition of blood, based on dissociation rates (87-99 %) of SR-colonies. While F. tularensis 15 NIIEG strain 1990 contains specified amounts (not less than 80 %) of immunogenic colonies if cultivated on nutrient media with the addition of blood, and fails to meet the requirements - if cultivated without. Identified in F. tularensis 15 NIIEG strain 1987 SR-colony decrement of 70-75 % in case of cultivation with or without addition of blood testifies to the deterioration of its immunogenic properties. RAPD and ERIC typing has showed high stability of the genome of F. tularensis 15 NIIEG cultures lyophilized at different times. Tularemia microbe vaccine strain has unique RAPD and ERIC profiles, insignificant alteration of which is observed upon storage of pathogen subculture in the dried from. |
| first_indexed | 2024-03-08T13:43:52Z |
| format | Article |
| id | doaj.art-d6a5c620f76f41598b945d43f2b0014f |
| institution | Directory Open Access Journal |
| issn | 0370-1069 2658-719X |
| language | Russian |
| last_indexed | 2025-02-17T14:09:18Z |
| publishDate | 2015-12-01 |
| publisher | Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” |
| record_format | Article |
| series | Проблемы особо опасных инфекций |
| spelling | doaj.art-d6a5c620f76f41598b945d43f2b0014f2024-12-24T12:09:52ZrusFederal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”Проблемы особо опасных инфекций0370-10692658-719X2015-12-0104919510.21055/0370-1069-2015-4-91-95273Characteristics of Phenotypic and Genetic Properties of <i>Francisella tularensis</i> 15 NIIEG Vaccine Strain with an Extended Storage PeriodE. A. Solov’Ev0L. V. Sayapina1N. A. Osina2D. S. Davydov3V. P. Bondarev4Scientific Center on Expertise of Medical Application ProductsScientific Center on Expertise of Medical Application ProductsRussian Research Anti-Plague Institute “Microbe”Scientific Center on Expertise of Medical Application ProductsScientific Center on Expertise of Medical Application ProductsInvestigated have been cultural-morphological, biochemical and genetic properties of lyophilized cultures of F. tularensis 15 NIIEG vaccine strain, accumulated within 60-years term and deposited at the State Collection of Pathogenic Microorganisms of Scientific Center on Expertise of Medical Application Products. The studies undertaken have demonstrated that storing of the strains in such a form at low temperatures, does not prevent changes of their genetic and phenotypic properties to the full extent. It is established that F. tularensis 15 NIIEG strain lyophilized in 1953, 1966, 1969, 2003 and 2012 maintains its immunogenic properties when cultivated on nutrient media Ft-agar with or without addition of blood, based on dissociation rates (87-99 %) of SR-colonies. While F. tularensis 15 NIIEG strain 1990 contains specified amounts (not less than 80 %) of immunogenic colonies if cultivated on nutrient media with the addition of blood, and fails to meet the requirements - if cultivated without. Identified in F. tularensis 15 NIIEG strain 1987 SR-colony decrement of 70-75 % in case of cultivation with or without addition of blood testifies to the deterioration of its immunogenic properties. RAPD and ERIC typing has showed high stability of the genome of F. tularensis 15 NIIEG cultures lyophilized at different times. Tularemia microbe vaccine strain has unique RAPD and ERIC profiles, insignificant alteration of which is observed upon storage of pathogen subculture in the dried from.https://journal.microbe.ru/jour/article/view/273вакцинный штамм francisella tularensis 15 нииэгкультурально-морфологическиебиохимические и генетические свойствадиссоциацияf tularensis 15 niieg vaccine straincultural-morphologicalbiochemical and genetic propertiesdissociation |
| spellingShingle | E. A. Solov’Ev L. V. Sayapina N. A. Osina D. S. Davydov V. P. Bondarev Characteristics of Phenotypic and Genetic Properties of <i>Francisella tularensis</i> 15 NIIEG Vaccine Strain with an Extended Storage Period Проблемы особо опасных инфекций вакцинный штамм francisella tularensis 15 нииэг культурально-морфологические биохимические и генетические свойства диссоциация f tularensis 15 niieg vaccine strain cultural-morphological biochemical and genetic properties dissociation |
| title | Characteristics of Phenotypic and Genetic Properties of <i>Francisella tularensis</i> 15 NIIEG Vaccine Strain with an Extended Storage Period |
| title_full | Characteristics of Phenotypic and Genetic Properties of <i>Francisella tularensis</i> 15 NIIEG Vaccine Strain with an Extended Storage Period |
| title_fullStr | Characteristics of Phenotypic and Genetic Properties of <i>Francisella tularensis</i> 15 NIIEG Vaccine Strain with an Extended Storage Period |
| title_full_unstemmed | Characteristics of Phenotypic and Genetic Properties of <i>Francisella tularensis</i> 15 NIIEG Vaccine Strain with an Extended Storage Period |
| title_short | Characteristics of Phenotypic and Genetic Properties of <i>Francisella tularensis</i> 15 NIIEG Vaccine Strain with an Extended Storage Period |
| title_sort | characteristics of phenotypic and genetic properties of i francisella tularensis i 15 niieg vaccine strain with an extended storage period |
| topic | вакцинный штамм francisella tularensis 15 нииэг культурально-морфологические биохимические и генетические свойства диссоциация f tularensis 15 niieg vaccine strain cultural-morphological biochemical and genetic properties dissociation |
| url | https://journal.microbe.ru/jour/article/view/273 |
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