Reprogramming macrophages via immune cell mobilized hydrogel microspheres for osteoarthritis treatments
Regulating macrophage activation precisely is crucial in treating chronic inflammation in osteoarthritis (OA). However, the stable pro-inflammatory state and deep distribution of macrophages in vivo pose a great challenge to treatment. In this study, inspired by the innate immune, immune cell mobili...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2024-02-01
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| Series: | Bioactive Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X23002931 |
| _version_ | 1797450941888200704 |
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| author | Pengcheng Xiao Xiaoyu Han Yanran Huang Jianye Yang Li Chen Zhengwei Cai Ning Hu Wenguo Cui Wei Huang |
| author_facet | Pengcheng Xiao Xiaoyu Han Yanran Huang Jianye Yang Li Chen Zhengwei Cai Ning Hu Wenguo Cui Wei Huang |
| author_sort | Pengcheng Xiao |
| collection | DOAJ |
| description | Regulating macrophage activation precisely is crucial in treating chronic inflammation in osteoarthritis (OA). However, the stable pro-inflammatory state and deep distribution of macrophages in vivo pose a great challenge to treatment. In this study, inspired by the innate immune, immune cell mobilized hydrogel microspheres were constructed by microfluidic methods and load chemokines, macrophage antibodies and engineered cell membrane vesicles (sEVs) via covalent and non-covalent junctions. The immune cell mobilized hydrogel microspheres, based on a mixture of streptavidin grafted hyaluronic acid methacrylate (HAMA-SA) and Chondroitin sulfate methacrylate (ChSMA) microspheres (HCM), can recruit, capture and reprogram proinflammatory macrophages in the joint cavity to improve the joint inflammatory microenvironment. In vitro experiments demonstrated that immune cell mobilized hydrogel microspheres had excellent macrophage recruitment, capture, and reprogramming abilities. Pro-inflammatory macrophages can be transformed into anti-inflammatory macrophages with an efficiency of 88.5 %. Animal experiments also revealed significant reduction in synovial inflammation and cartilage matrix degradation of OA. Therefore, the immune cell mobilized hydrogel microspheres may be an effective treatment of OA inflammation for the future. |
| first_indexed | 2024-03-09T14:46:53Z |
| format | Article |
| id | doaj.art-d6a76ee48a9e4586bddc67f64fab681f |
| institution | Directory Open Access Journal |
| issn | 2452-199X |
| language | English |
| last_indexed | 2024-03-09T14:46:53Z |
| publishDate | 2024-02-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Bioactive Materials |
| spelling | doaj.art-d6a76ee48a9e4586bddc67f64fab681f2023-11-27T04:15:00ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2024-02-0132242259Reprogramming macrophages via immune cell mobilized hydrogel microspheres for osteoarthritis treatmentsPengcheng Xiao0Xiaoyu Han1Yanran Huang2Jianye Yang3Li Chen4Zhengwei Cai5Ning Hu6Wenguo Cui7Wei Huang8Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Orthopaedic Research Laboratory, Chongqing Medical University, 400016, Chongqing, ChinaDepartment of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Orthopaedic Research Laboratory, Chongqing Medical University, 400016, Chongqing, China; Department of Orthopaedics, Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases, Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Ruijin 2nd Road, Shanghai, 200025, PR ChinaDepartment of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Orthopaedic Research Laboratory, Chongqing Medical University, 400016, Chongqing, ChinaDepartment of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Orthopaedic Research Laboratory, Chongqing Medical University, 400016, Chongqing, ChinaDepartment of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Orthopaedic Research Laboratory, Chongqing Medical University, 400016, Chongqing, ChinaDepartment of Orthopaedics, Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases, Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Ruijin 2nd Road, Shanghai, 200025, PR ChinaDepartment of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Orthopaedic Research Laboratory, Chongqing Medical University, 400016, Chongqing, China; Corresponding author.Department of Orthopaedics, Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases, Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Ruijin 2nd Road, Shanghai, 200025, PR China; Corresponding author.Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Orthopaedic Research Laboratory, Chongqing Medical University, 400016, Chongqing, China; Corresponding author.Regulating macrophage activation precisely is crucial in treating chronic inflammation in osteoarthritis (OA). However, the stable pro-inflammatory state and deep distribution of macrophages in vivo pose a great challenge to treatment. In this study, inspired by the innate immune, immune cell mobilized hydrogel microspheres were constructed by microfluidic methods and load chemokines, macrophage antibodies and engineered cell membrane vesicles (sEVs) via covalent and non-covalent junctions. The immune cell mobilized hydrogel microspheres, based on a mixture of streptavidin grafted hyaluronic acid methacrylate (HAMA-SA) and Chondroitin sulfate methacrylate (ChSMA) microspheres (HCM), can recruit, capture and reprogram proinflammatory macrophages in the joint cavity to improve the joint inflammatory microenvironment. In vitro experiments demonstrated that immune cell mobilized hydrogel microspheres had excellent macrophage recruitment, capture, and reprogramming abilities. Pro-inflammatory macrophages can be transformed into anti-inflammatory macrophages with an efficiency of 88.5 %. Animal experiments also revealed significant reduction in synovial inflammation and cartilage matrix degradation of OA. Therefore, the immune cell mobilized hydrogel microspheres may be an effective treatment of OA inflammation for the future.http://www.sciencedirect.com/science/article/pii/S2452199X23002931Engineered cell membrane vesiclesHydrogel microspheresMacrophage reprogramOsteoarthritis |
| spellingShingle | Pengcheng Xiao Xiaoyu Han Yanran Huang Jianye Yang Li Chen Zhengwei Cai Ning Hu Wenguo Cui Wei Huang Reprogramming macrophages via immune cell mobilized hydrogel microspheres for osteoarthritis treatments Bioactive Materials Engineered cell membrane vesicles Hydrogel microspheres Macrophage reprogram Osteoarthritis |
| title | Reprogramming macrophages via immune cell mobilized hydrogel microspheres for osteoarthritis treatments |
| title_full | Reprogramming macrophages via immune cell mobilized hydrogel microspheres for osteoarthritis treatments |
| title_fullStr | Reprogramming macrophages via immune cell mobilized hydrogel microspheres for osteoarthritis treatments |
| title_full_unstemmed | Reprogramming macrophages via immune cell mobilized hydrogel microspheres for osteoarthritis treatments |
| title_short | Reprogramming macrophages via immune cell mobilized hydrogel microspheres for osteoarthritis treatments |
| title_sort | reprogramming macrophages via immune cell mobilized hydrogel microspheres for osteoarthritis treatments |
| topic | Engineered cell membrane vesicles Hydrogel microspheres Macrophage reprogram Osteoarthritis |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X23002931 |
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