Transduced PEP-1-FK506BP ameliorates corneal injury in Botulinum toxin A-induced dry eye mouse model
FK506 binding protein 12 (FK506BP) belongs to a family ofimmunophilins, and is involved in multiple biologicalprocesses. However, the function of FK506BP in cornealdisease remains unclear. In this study, we examined theprotective effects on dry eye disease in a Botulinum toxin A(BTX-A) induced mouse...
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| Format: | Article |
| Language: | English |
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Korean Society for Biochemistry and Molecular Biology
2013-02-01
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| Series: | BMB Reports |
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| Online Access: | http://bmbreports.org/jbmb/pdf.php?data=MTMwOTI2MTdAcGRmX3JhaW50cmFjZV9sZWV5c0AlNUI0Ni0yJTVEMTMwMjI2MTkyMF8lMjgxMjQtMTI5JTI5Qk1CXzEyLTI3Mi5wZGY= |
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| author | Dae Won Kim Sung Ho Lee Sae Kwang Ku Soo Hyun Cho Sung-Woo Cho Ga Hyeon Yoon Hyun Sook Hwang Jinseu Park Won Sik Eum Oh-Shin Kwon Soo Young Choi |
| author_facet | Dae Won Kim Sung Ho Lee Sae Kwang Ku Soo Hyun Cho Sung-Woo Cho Ga Hyeon Yoon Hyun Sook Hwang Jinseu Park Won Sik Eum Oh-Shin Kwon Soo Young Choi |
| author_sort | Dae Won Kim |
| collection | DOAJ |
| description | FK506 binding protein 12 (FK506BP) belongs to a family ofimmunophilins, and is involved in multiple biologicalprocesses. However, the function of FK506BP in cornealdisease remains unclear. In this study, we examined theprotective effects on dry eye disease in a Botulinum toxin A(BTX-A) induced mouse model, using a cell-permeablePEP-1-FK506BP protein. PEP-1-FK506BP efficiently transducedinto human corneal epithelial cells in a time- anddose-dependent manner, and remained stable in the cells for48 h. In addition, we demonstrated that topical application ofPEP-1-FK506BP was transduced into mouse cornea andconjunctiva by immunohistochemistry. Furthermore, topicalapplication of PEP-1-FK506BP to BTX-A-induced mouse modelmarkedly inhibited expression levels of pro-inflammatorycytokines such as interleukin-1β (IL-1β), tumor necrosisfactor-α (TNF-α) and macrophage inhibitory factor (MIF) incorneal and conjunctival epithelium. These results suggestPEP-1-FK506BP as a potential therapeutic agent for dry eyediseases. [BMB Reports 2013; 46(2): 124-129] |
| first_indexed | 2024-12-23T14:57:11Z |
| format | Article |
| id | doaj.art-d6a7de7e84a94045a515e823c0955a23 |
| institution | Directory Open Access Journal |
| issn | 1976-6696 1976-670X |
| language | English |
| last_indexed | 2024-12-23T14:57:11Z |
| publishDate | 2013-02-01 |
| publisher | Korean Society for Biochemistry and Molecular Biology |
| record_format | Article |
| series | BMB Reports |
| spelling | doaj.art-d6a7de7e84a94045a515e823c0955a232022-12-21T17:42:44ZengKorean Society for Biochemistry and Molecular BiologyBMB Reports1976-66961976-670X2013-02-01462124129http://dx.doi.org/10.5483/BMBRep.2013.46.2.272Transduced PEP-1-FK506BP ameliorates corneal injury in Botulinum toxin A-induced dry eye mouse modelDae Won KimSung Ho LeeSae Kwang KuSoo Hyun ChoSung-Woo ChoGa Hyeon YoonHyun Sook HwangJinseu ParkWon Sik EumOh-Shin KwonSoo Young ChoiFK506 binding protein 12 (FK506BP) belongs to a family ofimmunophilins, and is involved in multiple biologicalprocesses. However, the function of FK506BP in cornealdisease remains unclear. In this study, we examined theprotective effects on dry eye disease in a Botulinum toxin A(BTX-A) induced mouse model, using a cell-permeablePEP-1-FK506BP protein. PEP-1-FK506BP efficiently transducedinto human corneal epithelial cells in a time- anddose-dependent manner, and remained stable in the cells for48 h. In addition, we demonstrated that topical application ofPEP-1-FK506BP was transduced into mouse cornea andconjunctiva by immunohistochemistry. Furthermore, topicalapplication of PEP-1-FK506BP to BTX-A-induced mouse modelmarkedly inhibited expression levels of pro-inflammatorycytokines such as interleukin-1β (IL-1β), tumor necrosisfactor-α (TNF-α) and macrophage inhibitory factor (MIF) incorneal and conjunctival epithelium. These results suggestPEP-1-FK506BP as a potential therapeutic agent for dry eyediseases. [BMB Reports 2013; 46(2): 124-129]http://bmbreports.org/jbmb/pdf.php?data=MTMwOTI2MTdAcGRmX3JhaW50cmFjZV9sZWV5c0AlNUI0Ni0yJTVEMTMwMjI2MTkyMF8lMjgxMjQtMTI5JTI5Qk1CXzEyLTI3Mi5wZGY=CytokinesDry eye diseaseInflammationPEP-1-FK506BPProtein therapy |
| spellingShingle | Dae Won Kim Sung Ho Lee Sae Kwang Ku Soo Hyun Cho Sung-Woo Cho Ga Hyeon Yoon Hyun Sook Hwang Jinseu Park Won Sik Eum Oh-Shin Kwon Soo Young Choi Transduced PEP-1-FK506BP ameliorates corneal injury in Botulinum toxin A-induced dry eye mouse model BMB Reports Cytokines Dry eye disease Inflammation PEP-1-FK506BP Protein therapy |
| title | Transduced PEP-1-FK506BP ameliorates corneal injury in Botulinum toxin A-induced dry eye mouse model |
| title_full | Transduced PEP-1-FK506BP ameliorates corneal injury in Botulinum toxin A-induced dry eye mouse model |
| title_fullStr | Transduced PEP-1-FK506BP ameliorates corneal injury in Botulinum toxin A-induced dry eye mouse model |
| title_full_unstemmed | Transduced PEP-1-FK506BP ameliorates corneal injury in Botulinum toxin A-induced dry eye mouse model |
| title_short | Transduced PEP-1-FK506BP ameliorates corneal injury in Botulinum toxin A-induced dry eye mouse model |
| title_sort | transduced pep 1 fk506bp ameliorates corneal injury in botulinum toxin a induced dry eye mouse model |
| topic | Cytokines Dry eye disease Inflammation PEP-1-FK506BP Protein therapy |
| url | http://bmbreports.org/jbmb/pdf.php?data=MTMwOTI2MTdAcGRmX3JhaW50cmFjZV9sZWV5c0AlNUI0Ni0yJTVEMTMwMjI2MTkyMF8lMjgxMjQtMTI5JTI5Qk1CXzEyLTI3Mi5wZGY= |
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