Optimization of Preparation of Casein-derived Cholesterol-lowering Peptide by Enzymatic Hydrolysis
Casein was hydrolyzed by neutral protease, alkaline protease and trypsin to determine the best protease for preparation of cholesterol-lowering peptide. The effects of hydrolysis pH value, hydrolysis temperature, enzyme to substrate ratio, substrate concentration and hydrolysis time on casein hydrol...
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2022-10-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022010165 |
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| author | Xiaohui LIANG Xiaozhi WANG Jiayuan ZHAO Tingfei JIN Xu LI Mengfan LUO Donghu TAN Mingzhen LIU Haibo LUO Yuxing GUO |
| author_facet | Xiaohui LIANG Xiaozhi WANG Jiayuan ZHAO Tingfei JIN Xu LI Mengfan LUO Donghu TAN Mingzhen LIU Haibo LUO Yuxing GUO |
| author_sort | Xiaohui LIANG |
| collection | DOAJ |
| description | Casein was hydrolyzed by neutral protease, alkaline protease and trypsin to determine the best protease for preparation of cholesterol-lowering peptide. The effects of hydrolysis pH value, hydrolysis temperature, enzyme to substrate ratio, substrate concentration and hydrolysis time on casein hydrolysis degree and cholesterol micelle solubility inhibition rate were investigated by single factor and response surface experiments, and the optimal hydrolysis conditions were determined. Then the separation process of cholesterol-lowering peptide was determined by ultrafiltration and gel filtration chromatography. The results showed that the optimal enzyme was neutral protease, and the optimal hydrolysis conditions were as follows: Reaction temperature 51.3 ℃, enzyme to substrate concentration ratio 6.47%, pH6.34, substrate concentration 5 g/100 mL, reaction time 3.5 h , and cholesterol inhibition rate 58.25%±0.59%; The separation conditions of casein cholesterol-lowering peptide by Sephadex G-10 were as follows: Loading concentration 80 mg/mL, loading volume 2.5 mL, elution rate 3.5 mL/min; The inhibition rates of cholesterol solubility of peak 1 and peak 2 samples were 24.2%±0.24% and 4.3%±0.16% at 100 µg/mL after ultrafiltration and chromatography. |
| first_indexed | 2024-04-11T16:10:05Z |
| format | Article |
| id | doaj.art-d6ab914ff2d14edcb4045fe1493b0016 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-11T16:10:05Z |
| publishDate | 2022-10-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-d6ab914ff2d14edcb4045fe1493b00162022-12-22T04:14:43ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-10-01431928028710.13386/j.issn1002-0306.20220101652022010165-19Optimization of Preparation of Casein-derived Cholesterol-lowering Peptide by Enzymatic HydrolysisXiaohui LIANG0Xiaozhi WANG1Jiayuan ZHAO2Tingfei JIN3Xu LI4Mengfan LUO5Donghu TAN6Mingzhen LIU7Haibo LUO8Yuxing GUO9College of Food and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210000, ChinaCollege of Food and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210000, ChinaCollege of Food and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210000, ChinaGuangdong Yikewei Bio-Technology Co., Ltd., Guangzhou 510000, ChinaGuangdong Yikewei Bio-Technology Co., Ltd., Guangzhou 510000, ChinaCollege of Food and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210000, ChinaCollege of Food and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210000, ChinaCollege of Food and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210000, ChinaCollege of Food and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210000, ChinaCollege of Food and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210000, ChinaCasein was hydrolyzed by neutral protease, alkaline protease and trypsin to determine the best protease for preparation of cholesterol-lowering peptide. The effects of hydrolysis pH value, hydrolysis temperature, enzyme to substrate ratio, substrate concentration and hydrolysis time on casein hydrolysis degree and cholesterol micelle solubility inhibition rate were investigated by single factor and response surface experiments, and the optimal hydrolysis conditions were determined. Then the separation process of cholesterol-lowering peptide was determined by ultrafiltration and gel filtration chromatography. The results showed that the optimal enzyme was neutral protease, and the optimal hydrolysis conditions were as follows: Reaction temperature 51.3 ℃, enzyme to substrate concentration ratio 6.47%, pH6.34, substrate concentration 5 g/100 mL, reaction time 3.5 h , and cholesterol inhibition rate 58.25%±0.59%; The separation conditions of casein cholesterol-lowering peptide by Sephadex G-10 were as follows: Loading concentration 80 mg/mL, loading volume 2.5 mL, elution rate 3.5 mL/min; The inhibition rates of cholesterol solubility of peak 1 and peak 2 samples were 24.2%±0.24% and 4.3%±0.16% at 100 µg/mL after ultrafiltration and chromatography.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022010165caseinproteasecholesterol-lowering peptidesresponse face method |
| spellingShingle | Xiaohui LIANG Xiaozhi WANG Jiayuan ZHAO Tingfei JIN Xu LI Mengfan LUO Donghu TAN Mingzhen LIU Haibo LUO Yuxing GUO Optimization of Preparation of Casein-derived Cholesterol-lowering Peptide by Enzymatic Hydrolysis Shipin gongye ke-ji casein protease cholesterol-lowering peptides response face method |
| title | Optimization of Preparation of Casein-derived Cholesterol-lowering Peptide by Enzymatic Hydrolysis |
| title_full | Optimization of Preparation of Casein-derived Cholesterol-lowering Peptide by Enzymatic Hydrolysis |
| title_fullStr | Optimization of Preparation of Casein-derived Cholesterol-lowering Peptide by Enzymatic Hydrolysis |
| title_full_unstemmed | Optimization of Preparation of Casein-derived Cholesterol-lowering Peptide by Enzymatic Hydrolysis |
| title_short | Optimization of Preparation of Casein-derived Cholesterol-lowering Peptide by Enzymatic Hydrolysis |
| title_sort | optimization of preparation of casein derived cholesterol lowering peptide by enzymatic hydrolysis |
| topic | casein protease cholesterol-lowering peptides response face method |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022010165 |
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