Application of CRISPR-Based C-to-G Base editing in rice protoplasts
Abstract Recently, new types of base editors, C-to-G base editors (CGBEs), that enable cytosine transversions that are unachievable with cytosine base editors (CBEs) and adenosine base editors (ABEs), have been developed in human cells. However, despite their importance in crop genome editing, the e...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2023-03-01
|
| Series: | Applied Biological Chemistry |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13765-023-00775-5 |
| _version_ | 1797863833774063616 |
|---|---|
| author | Jimin Lee Nuri Oh Jae-Young Yun Hee Soon Choi Jang-Kyun Seo Jin-Ho Kang Choonkyun Jung |
| author_facet | Jimin Lee Nuri Oh Jae-Young Yun Hee Soon Choi Jang-Kyun Seo Jin-Ho Kang Choonkyun Jung |
| author_sort | Jimin Lee |
| collection | DOAJ |
| description | Abstract Recently, new types of base editors, C-to-G base editors (CGBEs), that enable cytosine transversions that are unachievable with cytosine base editors (CBEs) and adenosine base editors (ABEs), have been developed in human cells. However, despite their importance in crop genome editing, the efficacy of CGBEs has not yet been extensively evaluated. In our study, based on the previously reported plant-compatible CBE and human CGBE, we demonstrated that our monocot plant-compatible CGBEs (PcCGBEs) enable cytosine transversions (C-to-G) in rice protoplasts. For all targets tested, PcCGBEs (monocot plant-compatible CGBEs) appeared to have substantial levels of C-to-G editing activity. PcCGBE showed a much higher C-to-G base editing activity and C-to-G specificity among C-to-D conversions than the mini-version of PcCGBE. Our demonstration of PcCGBE could provide a platform for the further development of enhanced CGBEs for reliable application as a new crop breeding technology. |
| first_indexed | 2024-04-09T22:42:00Z |
| format | Article |
| id | doaj.art-d6ab95374721459cb389b6e9774de82c |
| institution | Directory Open Access Journal |
| issn | 2468-0842 |
| language | English |
| last_indexed | 2024-04-09T22:42:00Z |
| publishDate | 2023-03-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Applied Biological Chemistry |
| spelling | doaj.art-d6ab95374721459cb389b6e9774de82c2023-03-22T12:08:32ZengSpringerOpenApplied Biological Chemistry2468-08422023-03-016611510.1186/s13765-023-00775-5Application of CRISPR-Based C-to-G Base editing in rice protoplastsJimin Lee0Nuri Oh1Jae-Young Yun2Hee Soon Choi3Jang-Kyun Seo4Jin-Ho Kang5Choonkyun Jung6Department of International Agricultural Technology, Seoul National UniversityDepartment of Agriculture, Forestry, and Bioresources, College of Agriculture and Life Sciences, Seoul National UniversityCrop Biotechnology Institute, Institutes of Green Bio Science & Technology, Seoul National UniversityCrop Biotechnology Institute, Institutes of Green Bio Science & Technology, Seoul National UniversityDepartment of International Agricultural Technology, Seoul National UniversityDepartment of International Agricultural Technology, Seoul National UniversityDepartment of International Agricultural Technology, Seoul National UniversityAbstract Recently, new types of base editors, C-to-G base editors (CGBEs), that enable cytosine transversions that are unachievable with cytosine base editors (CBEs) and adenosine base editors (ABEs), have been developed in human cells. However, despite their importance in crop genome editing, the efficacy of CGBEs has not yet been extensively evaluated. In our study, based on the previously reported plant-compatible CBE and human CGBE, we demonstrated that our monocot plant-compatible CGBEs (PcCGBEs) enable cytosine transversions (C-to-G) in rice protoplasts. For all targets tested, PcCGBEs (monocot plant-compatible CGBEs) appeared to have substantial levels of C-to-G editing activity. PcCGBE showed a much higher C-to-G base editing activity and C-to-G specificity among C-to-D conversions than the mini-version of PcCGBE. Our demonstration of PcCGBE could provide a platform for the further development of enhanced CGBEs for reliable application as a new crop breeding technology.https://doi.org/10.1186/s13765-023-00775-5CRISPR/Cas9Cytosine deaminaseUracil glycosylaseBase editingC-to-GCGBE |
| spellingShingle | Jimin Lee Nuri Oh Jae-Young Yun Hee Soon Choi Jang-Kyun Seo Jin-Ho Kang Choonkyun Jung Application of CRISPR-Based C-to-G Base editing in rice protoplasts Applied Biological Chemistry CRISPR/Cas9 Cytosine deaminase Uracil glycosylase Base editing C-to-G CGBE |
| title | Application of CRISPR-Based C-to-G Base editing in rice protoplasts |
| title_full | Application of CRISPR-Based C-to-G Base editing in rice protoplasts |
| title_fullStr | Application of CRISPR-Based C-to-G Base editing in rice protoplasts |
| title_full_unstemmed | Application of CRISPR-Based C-to-G Base editing in rice protoplasts |
| title_short | Application of CRISPR-Based C-to-G Base editing in rice protoplasts |
| title_sort | application of crispr based c to g base editing in rice protoplasts |
| topic | CRISPR/Cas9 Cytosine deaminase Uracil glycosylase Base editing C-to-G CGBE |
| url | https://doi.org/10.1186/s13765-023-00775-5 |
| work_keys_str_mv | AT jiminlee applicationofcrisprbasedctogbaseeditinginriceprotoplasts AT nurioh applicationofcrisprbasedctogbaseeditinginriceprotoplasts AT jaeyoungyun applicationofcrisprbasedctogbaseeditinginriceprotoplasts AT heesoonchoi applicationofcrisprbasedctogbaseeditinginriceprotoplasts AT jangkyunseo applicationofcrisprbasedctogbaseeditinginriceprotoplasts AT jinhokang applicationofcrisprbasedctogbaseeditinginriceprotoplasts AT choonkyunjung applicationofcrisprbasedctogbaseeditinginriceprotoplasts |