miR-499 inhibits migration and promotes apoptosis of human osteosarcoma cell line Saos2 by targeting at TGF-α
Objective To explore the role of miR-499 targeting TGF-α mRNA protein in regulating the migration and apoptosis of human osteosarcoma cell line Saos2 and its pathogenesis. Methods The expression differences of miR-499 and TGF-α in osteosarcoma tissues were detected by RT-qPCR. Double luciferase repo...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2022-08-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2022-42-8-1230.pdf |
| _version_ | 1797365885382426624 |
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| author | WANG Lei, QIU Ming-xian, ZHANG Hui-rong, ZHANG Jin-ping, ZHAO Jing, KANG Xiao, ZHANG Qing-quan |
| author_facet | WANG Lei, QIU Ming-xian, ZHANG Hui-rong, ZHANG Jin-ping, ZHAO Jing, KANG Xiao, ZHANG Qing-quan |
| author_sort | WANG Lei, QIU Ming-xian, ZHANG Hui-rong, ZHANG Jin-ping, ZHAO Jing, KANG Xiao, ZHANG Qing-quan |
| collection | DOAJ |
| description | Objective To explore the role of miR-499 targeting TGF-α mRNA protein in regulating the migration and apoptosis of human osteosarcoma cell line Saos2 and its pathogenesis. Methods The expression differences of miR-499 and TGF-α in osteosarcoma tissues were detected by RT-qPCR. Double luciferase reporter gene assay was used to detect the targeting relationship between miR-499 and TGF-α. Transwell cell assay and TUNEL apoptosis assay were used to detect the migration and apoptosis of Saos2 cells. A nude mouse xenograft model of osteosarcoma was constructed to detect the regulatory mechanism of miR-499/TGF-α axis in vivo. Results In osteosarcoma, miR-499 expression was decreased (P＜0.01) but TGF-α mRNA expression was increased(P＜0.01). miR-499 negatively regulated TGF-α mRNA protein expression (P＜0.01). Transfection of miR-499 simulacrum inhibited the migration and promoted apoptosis of Saos2 cells (P＜0.01), while TGF-α promoted the migration and inhibited apoptosis of Saos2 cells (P＜0.01). miR-499 inhibited the carcinogenic effect of TGF-α. Conclusions miR-499 inhibits the biological function of osteosarcoma cell line Saos2 by inhibiting at TGF-α mRNA protein level which suggests that miR-499 may inhibit the development of osteosarcoma. |
| first_indexed | 2024-03-08T16:55:21Z |
| format | Article |
| id | doaj.art-d6b3797e92d1420f8e0939b061fc1c99 |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T16:55:21Z |
| publishDate | 2022-08-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-d6b3797e92d1420f8e0939b061fc1c992024-01-05T02:36:08ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252022-08-014281230123610.16352/j.issn.1001-6325.2022.08.1230miR-499 inhibits migration and promotes apoptosis of human osteosarcoma cell line Saos2 by targeting at TGF-αWANG Lei, QIU Ming-xian, ZHANG Hui-rong, ZHANG Jin-ping, ZHAO Jing, KANG Xiao, ZHANG Qing-quan01. the Fourth People's Hospital of Hengshui, Hengshui 053000; ;2. Hengshui People's Hospital of Hebei Province,Hengshui 053000, ChinaObjective To explore the role of miR-499 targeting TGF-α mRNA protein in regulating the migration and apoptosis of human osteosarcoma cell line Saos2 and its pathogenesis. Methods The expression differences of miR-499 and TGF-α in osteosarcoma tissues were detected by RT-qPCR. Double luciferase reporter gene assay was used to detect the targeting relationship between miR-499 and TGF-α. Transwell cell assay and TUNEL apoptosis assay were used to detect the migration and apoptosis of Saos2 cells. A nude mouse xenograft model of osteosarcoma was constructed to detect the regulatory mechanism of miR-499/TGF-α axis in vivo. Results In osteosarcoma, miR-499 expression was decreased (P＜0.01) but TGF-α mRNA expression was increased(P＜0.01). miR-499 negatively regulated TGF-α mRNA protein expression (P＜0.01). Transfection of miR-499 simulacrum inhibited the migration and promoted apoptosis of Saos2 cells (P＜0.01), while TGF-α promoted the migration and inhibited apoptosis of Saos2 cells (P＜0.01). miR-499 inhibited the carcinogenic effect of TGF-α. Conclusions miR-499 inhibits the biological function of osteosarcoma cell line Saos2 by inhibiting at TGF-α mRNA protein level which suggests that miR-499 may inhibit the development of osteosarcoma.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2022-42-8-1230.pdfosteosarcoma|mir-499|tgf-α|saos2 |
| spellingShingle | WANG Lei, QIU Ming-xian, ZHANG Hui-rong, ZHANG Jin-ping, ZHAO Jing, KANG Xiao, ZHANG Qing-quan miR-499 inhibits migration and promotes apoptosis of human osteosarcoma cell line Saos2 by targeting at TGF-α Jichu yixue yu linchuang osteosarcoma|mir-499|tgf-α|saos2 |
| title | miR-499 inhibits migration and promotes apoptosis of human osteosarcoma cell line Saos2 by targeting at TGF-α |
| title_full | miR-499 inhibits migration and promotes apoptosis of human osteosarcoma cell line Saos2 by targeting at TGF-α |
| title_fullStr | miR-499 inhibits migration and promotes apoptosis of human osteosarcoma cell line Saos2 by targeting at TGF-α |
| title_full_unstemmed | miR-499 inhibits migration and promotes apoptosis of human osteosarcoma cell line Saos2 by targeting at TGF-α |
| title_short | miR-499 inhibits migration and promotes apoptosis of human osteosarcoma cell line Saos2 by targeting at TGF-α |
| title_sort | mir 499 inhibits migration and promotes apoptosis of human osteosarcoma cell line saos2 by targeting at tgf α |
| topic | osteosarcoma|mir-499|tgf-α|saos2 |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2022-42-8-1230.pdf |
| work_keys_str_mv | AT wangleiqiumingxianzhanghuirongzhangjinpingzhaojingkangxiaozhangqingquan mir499inhibitsmigrationandpromotesapoptosisofhumanosteosarcomacelllinesaos2bytargetingattgfa |