FEN1 inhibitor increases sensitivity of radiotherapy in cervical cancer cells
Abstract Background Cervical cancer is one of the most common causes of cancer‐associated mortality among affected women in the world. At present, treatment with weekly cisplatin plus ionizing radiation (IR) therapy is the standard regimen for cervical cancer, especially for locally advanced cervica...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-12-01
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| Series: | Cancer Medicine |
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| Online Access: | https://doi.org/10.1002/cam4.2615 |
| _version_ | 1830287642323845120 |
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| author | Jin‐Li Li Jian‐Ping Wang Hong Chang Sheng‐Ming Deng Jia‐Hui Du Xiao‐Xiao Wang He‐Juan Hu Dong‐Yin Li Xiang‐Bin Xu Wei‐Qiang Guo Yao‐Hua Song Zhigang Guo Min‐Xuan Sun Yi‐Wei Wu Song‐Bai Liu |
| author_facet | Jin‐Li Li Jian‐Ping Wang Hong Chang Sheng‐Ming Deng Jia‐Hui Du Xiao‐Xiao Wang He‐Juan Hu Dong‐Yin Li Xiang‐Bin Xu Wei‐Qiang Guo Yao‐Hua Song Zhigang Guo Min‐Xuan Sun Yi‐Wei Wu Song‐Bai Liu |
| author_sort | Jin‐Li Li |
| collection | DOAJ |
| description | Abstract Background Cervical cancer is one of the most common causes of cancer‐associated mortality among affected women in the world. At present, treatment with weekly cisplatin plus ionizing radiation (IR) therapy is the standard regimen for cervical cancer, especially for locally advanced cervical cancer. The purpose of this study is to determine whether FEN1 inhibitors could enhance the therapeutic effect of IR therapy. Methods Western blot was applied to determine the expression of FEN1‐ and apoptosis‐related proteins. Cell growth inhibition assay and colony formation assay were used to determine the effects of FEN1 inhibitor and IR exposure for Hela cells in vitro. CRISPR technology was used to knockdown FEN1 expression level of 293T cells, and tumor xenograft in nude mice was employed to determine the effects of FEN1 inhibitor and IR exposure on tumor growth in vivo. Results Our data revealed that FEN1 is overexpressed in HeLa cell and can be upregulated further by IR. We also demonstrated that FEN1 inhibitor enhances IR sensitivity of cervical cancer in vitro and in vivo. Conclusion FEN1 inhibitor SC13 could sensitize radiotherapy of cervical cancer cell. |
| first_indexed | 2024-12-19T04:20:19Z |
| format | Article |
| id | doaj.art-d6b59cec6b334666bb97c016552c2a22 |
| institution | Directory Open Access Journal |
| issn | 2045-7634 |
| language | English |
| last_indexed | 2024-12-19T04:20:19Z |
| publishDate | 2019-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cancer Medicine |
| spelling | doaj.art-d6b59cec6b334666bb97c016552c2a222022-12-21T20:36:10ZengWileyCancer Medicine2045-76342019-12-018187774778010.1002/cam4.2615FEN1 inhibitor increases sensitivity of radiotherapy in cervical cancer cellsJin‐Li Li0Jian‐Ping Wang1Hong Chang2Sheng‐Ming Deng3Jia‐Hui Du4Xiao‐Xiao Wang5He‐Juan Hu6Dong‐Yin Li7Xiang‐Bin Xu8Wei‐Qiang Guo9Yao‐Hua Song10Zhigang Guo11Min‐Xuan Sun12Yi‐Wei Wu13Song‐Bai Liu14Department of Radiation Oncology The Affiliated Hospital of Soochow University Suzhou ChinaDepartment of Radiation Oncology The Affiliated Hospital of Soochow University Suzhou ChinaSuzhou Key Laboratory for Medical Biotechnology Suzhou Vocational Health College Suzhou ChinaDepartment of Nuclear Medicine The Affiliated Hospital of Soochow University Suzhou ChinaSuzhou Key Laboratory for Medical Biotechnology Suzhou Vocational Health College Suzhou ChinaSuzhou Key Laboratory for Medical Biotechnology Suzhou Vocational Health College Suzhou ChinaSuzhou Key Laboratory for Medical Biotechnology Suzhou Vocational Health College Suzhou ChinaSuzhou Key Laboratory for Medical Biotechnology Suzhou Vocational Health College Suzhou ChinaCollege of Food Science and Technology Hainan University Haikou ChinaSchool of Chemistry, Biology and Materials Engineering Suzhou University of Science and Technology Suzhou ChinaCyrus Tang Hematology Center Collaborative Innovation Center of Hematology Soochow University Suzhou ChinaJiangsu Key Laboratory for Molecular and Medical Biotechnology College of Life Science Nanjing Normal University Nanjing ChinaJiangsu Key Laboratory of Medical Optics Suzhou Institute of Biomedical Engineering and Technology Chinese Academy of Sciences Suzhou ChinaDepartment of Nuclear Medicine The Affiliated Hospital of Soochow University Suzhou ChinaSuzhou Key Laboratory for Medical Biotechnology Suzhou Vocational Health College Suzhou ChinaAbstract Background Cervical cancer is one of the most common causes of cancer‐associated mortality among affected women in the world. At present, treatment with weekly cisplatin plus ionizing radiation (IR) therapy is the standard regimen for cervical cancer, especially for locally advanced cervical cancer. The purpose of this study is to determine whether FEN1 inhibitors could enhance the therapeutic effect of IR therapy. Methods Western blot was applied to determine the expression of FEN1‐ and apoptosis‐related proteins. Cell growth inhibition assay and colony formation assay were used to determine the effects of FEN1 inhibitor and IR exposure for Hela cells in vitro. CRISPR technology was used to knockdown FEN1 expression level of 293T cells, and tumor xenograft in nude mice was employed to determine the effects of FEN1 inhibitor and IR exposure on tumor growth in vivo. Results Our data revealed that FEN1 is overexpressed in HeLa cell and can be upregulated further by IR. We also demonstrated that FEN1 inhibitor enhances IR sensitivity of cervical cancer in vitro and in vivo. Conclusion FEN1 inhibitor SC13 could sensitize radiotherapy of cervical cancer cell.https://doi.org/10.1002/cam4.2615cervical cancerFEN1radiotherapytargeted therapy |
| spellingShingle | Jin‐Li Li Jian‐Ping Wang Hong Chang Sheng‐Ming Deng Jia‐Hui Du Xiao‐Xiao Wang He‐Juan Hu Dong‐Yin Li Xiang‐Bin Xu Wei‐Qiang Guo Yao‐Hua Song Zhigang Guo Min‐Xuan Sun Yi‐Wei Wu Song‐Bai Liu FEN1 inhibitor increases sensitivity of radiotherapy in cervical cancer cells Cancer Medicine cervical cancer FEN1 radiotherapy targeted therapy |
| title | FEN1 inhibitor increases sensitivity of radiotherapy in cervical cancer cells |
| title_full | FEN1 inhibitor increases sensitivity of radiotherapy in cervical cancer cells |
| title_fullStr | FEN1 inhibitor increases sensitivity of radiotherapy in cervical cancer cells |
| title_full_unstemmed | FEN1 inhibitor increases sensitivity of radiotherapy in cervical cancer cells |
| title_short | FEN1 inhibitor increases sensitivity of radiotherapy in cervical cancer cells |
| title_sort | fen1 inhibitor increases sensitivity of radiotherapy in cervical cancer cells |
| topic | cervical cancer FEN1 radiotherapy targeted therapy |
| url | https://doi.org/10.1002/cam4.2615 |
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