Genetic and physiological analysis of iron biofortification in maize kernels.
<h4>Background</h4>Maize is a major cereal crop widely consumed in developing countries, which have a high prevalence of iron (Fe) deficiency anemia. The major cause of Fe deficiency in these countries is inadequate intake of bioavailable Fe, where poverty is a major factor. Therefore, b...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2011-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21687662/pdf/?tool=EBI |
| _version_ | 1818435949744357376 |
|---|---|
| author | Mercy G Lung'aho Angela M Mwaniki Stephen J Szalma Jonathan J Hart Michael A Rutzke Leon V Kochian Raymond P Glahn Owen A Hoekenga |
| author_facet | Mercy G Lung'aho Angela M Mwaniki Stephen J Szalma Jonathan J Hart Michael A Rutzke Leon V Kochian Raymond P Glahn Owen A Hoekenga |
| author_sort | Mercy G Lung'aho |
| collection | DOAJ |
| description | <h4>Background</h4>Maize is a major cereal crop widely consumed in developing countries, which have a high prevalence of iron (Fe) deficiency anemia. The major cause of Fe deficiency in these countries is inadequate intake of bioavailable Fe, where poverty is a major factor. Therefore, biofortification of maize by increasing Fe concentration and or bioavailability has great potential to alleviate this deficiency. Maize is also a model system for genomic research and thus allows the opportunity for gene discovery. Here we describe an integrated genetic and physiological analysis of Fe nutrition in maize kernels, to identify loci that influence grain Fe concentration and bioavailability.<h4>Methodology</h4>Quantitative trait locus (QTL) analysis was used to dissect grain Fe concentration (FeGC) and Fe bioavailability (FeGB) from the Intermated B73 × Mo17 (IBM) recombinant inbred (RI) population. FeGC was determined by ion coupled argon plasma emission spectroscopy (ICP). FeGB was determined by an in vitro digestion/Caco-2 cell line bioassay.<h4>Conclusions</h4>Three modest QTL for FeGC were detected, in spite of high heritability. This suggests that FeGC is controlled by many small QTL, which may make it a challenging trait to improve by marker assisted breeding. Ten QTL for FeGB were identified and explained 54% of the variance observed in samples from a single year/location. Three of the largest FeGB QTL were isolated in sister derived lines and their effect was observed in three subsequent seasons in New York. Single season evaluations were also made at six other sites around North America, suggesting the enhancement of FeGB was not specific to our farm site. FeGB was not correlated with FeGC or phytic acid, suggesting that novel regulators of Fe nutrition are responsible for the differences observed. Our results indicate that iron biofortification of maize grain is achievable using specialized phenotyping tools and conventional plant breeding techniques. |
| first_indexed | 2024-12-14T17:01:01Z |
| format | Article |
| id | doaj.art-d6b61dd2c0b846fbae709b9d9f3ccf65 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T17:01:01Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-d6b61dd2c0b846fbae709b9d9f3ccf652022-12-21T22:53:50ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0166e2042910.1371/journal.pone.0020429Genetic and physiological analysis of iron biofortification in maize kernels.Mercy G Lung'ahoAngela M MwanikiStephen J SzalmaJonathan J HartMichael A RutzkeLeon V KochianRaymond P GlahnOwen A Hoekenga<h4>Background</h4>Maize is a major cereal crop widely consumed in developing countries, which have a high prevalence of iron (Fe) deficiency anemia. The major cause of Fe deficiency in these countries is inadequate intake of bioavailable Fe, where poverty is a major factor. Therefore, biofortification of maize by increasing Fe concentration and or bioavailability has great potential to alleviate this deficiency. Maize is also a model system for genomic research and thus allows the opportunity for gene discovery. Here we describe an integrated genetic and physiological analysis of Fe nutrition in maize kernels, to identify loci that influence grain Fe concentration and bioavailability.<h4>Methodology</h4>Quantitative trait locus (QTL) analysis was used to dissect grain Fe concentration (FeGC) and Fe bioavailability (FeGB) from the Intermated B73 × Mo17 (IBM) recombinant inbred (RI) population. FeGC was determined by ion coupled argon plasma emission spectroscopy (ICP). FeGB was determined by an in vitro digestion/Caco-2 cell line bioassay.<h4>Conclusions</h4>Three modest QTL for FeGC were detected, in spite of high heritability. This suggests that FeGC is controlled by many small QTL, which may make it a challenging trait to improve by marker assisted breeding. Ten QTL for FeGB were identified and explained 54% of the variance observed in samples from a single year/location. Three of the largest FeGB QTL were isolated in sister derived lines and their effect was observed in three subsequent seasons in New York. Single season evaluations were also made at six other sites around North America, suggesting the enhancement of FeGB was not specific to our farm site. FeGB was not correlated with FeGC or phytic acid, suggesting that novel regulators of Fe nutrition are responsible for the differences observed. Our results indicate that iron biofortification of maize grain is achievable using specialized phenotyping tools and conventional plant breeding techniques.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21687662/pdf/?tool=EBI |
| spellingShingle | Mercy G Lung'aho Angela M Mwaniki Stephen J Szalma Jonathan J Hart Michael A Rutzke Leon V Kochian Raymond P Glahn Owen A Hoekenga Genetic and physiological analysis of iron biofortification in maize kernels. PLoS ONE |
| title | Genetic and physiological analysis of iron biofortification in maize kernels. |
| title_full | Genetic and physiological analysis of iron biofortification in maize kernels. |
| title_fullStr | Genetic and physiological analysis of iron biofortification in maize kernels. |
| title_full_unstemmed | Genetic and physiological analysis of iron biofortification in maize kernels. |
| title_short | Genetic and physiological analysis of iron biofortification in maize kernels. |
| title_sort | genetic and physiological analysis of iron biofortification in maize kernels |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21687662/pdf/?tool=EBI |
| work_keys_str_mv | AT mercyglungaho geneticandphysiologicalanalysisofironbiofortificationinmaizekernels AT angelammwaniki geneticandphysiologicalanalysisofironbiofortificationinmaizekernels AT stephenjszalma geneticandphysiologicalanalysisofironbiofortificationinmaizekernels AT jonathanjhart geneticandphysiologicalanalysisofironbiofortificationinmaizekernels AT michaelarutzke geneticandphysiologicalanalysisofironbiofortificationinmaizekernels AT leonvkochian geneticandphysiologicalanalysisofironbiofortificationinmaizekernels AT raymondpglahn geneticandphysiologicalanalysisofironbiofortificationinmaizekernels AT owenahoekenga geneticandphysiologicalanalysisofironbiofortificationinmaizekernels |