Municipal and neighbourhood level wastewater surveillance and subtyping of an influenza virus outbreak
Abstract Recurrent influenza epidemics and pandemic potential are significant risks to global health. Public health authorities use clinical surveillance to locate and monitor influenza and influenza-like cases and outbreaks to mitigate hospitalizations and deaths. Currently, global integration of c...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-09-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-20076-z |
| _version_ | 1798001431773446144 |
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| author | Elisabeth Mercier Patrick M. D’Aoust Ocean Thakali Nada Hegazy Jian-Jun Jia Zhihao Zhang Walaa Eid Julio Plaza-Diaz Md Pervez Kabir Wanting Fang Aaron Cowan Sean E. Stephenson Lakshmi Pisharody Alex E. MacKenzie Tyson E. Graber Shen Wan Robert Delatolla |
| author_facet | Elisabeth Mercier Patrick M. D’Aoust Ocean Thakali Nada Hegazy Jian-Jun Jia Zhihao Zhang Walaa Eid Julio Plaza-Diaz Md Pervez Kabir Wanting Fang Aaron Cowan Sean E. Stephenson Lakshmi Pisharody Alex E. MacKenzie Tyson E. Graber Shen Wan Robert Delatolla |
| author_sort | Elisabeth Mercier |
| collection | DOAJ |
| description | Abstract Recurrent influenza epidemics and pandemic potential are significant risks to global health. Public health authorities use clinical surveillance to locate and monitor influenza and influenza-like cases and outbreaks to mitigate hospitalizations and deaths. Currently, global integration of clinical surveillance is the only reliable method for reporting influenza types and subtypes to warn of emergent pandemic strains. The utility of wastewater surveillance (WWS) during the COVID-19 pandemic as a less resource intensive replacement or complement for clinical surveillance has been predicated on analyzing viral fragments in wastewater. We show here that influenza virus targets are stable in wastewater and partitions favorably to the solids fraction. By quantifying, typing, and subtyping the virus in municipal wastewater and primary sludge during a community outbreak, we forecasted a citywide flu outbreak with a 17-day lead time and provided population-level viral subtyping in near real-time to show the feasibility of influenza virus WWS at the municipal and neighbourhood levels in near real time using minimal resources and infrastructure. |
| first_indexed | 2024-04-11T11:36:08Z |
| format | Article |
| id | doaj.art-d6bcfdf0183d4a909dd029cb788eb227 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T11:36:08Z |
| publishDate | 2022-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-d6bcfdf0183d4a909dd029cb788eb2272022-12-22T04:25:58ZengNature PortfolioScientific Reports2045-23222022-09-0112111110.1038/s41598-022-20076-zMunicipal and neighbourhood level wastewater surveillance and subtyping of an influenza virus outbreakElisabeth Mercier0Patrick M. D’Aoust1Ocean Thakali2Nada Hegazy3Jian-Jun Jia4Zhihao Zhang5Walaa Eid6Julio Plaza-Diaz7Md Pervez Kabir8Wanting Fang9Aaron Cowan10Sean E. Stephenson11Lakshmi Pisharody12Alex E. MacKenzie13Tyson E. Graber14Shen Wan15Robert Delatolla16Department of Civil Engineering, University of OttawaDepartment of Civil Engineering, University of OttawaDepartment of Civil Engineering, University of OttawaDepartment of Civil Engineering, University of OttawaDepartment of Civil Engineering, University of OttawaDepartment of Civil Engineering, University of OttawaChildren’s Hospital of Eastern Ontario Research InstituteChildren’s Hospital of Eastern Ontario Research InstituteDepartment of Civil Engineering, University of OttawaDepartment of Civil Engineering, University of OttawaDepartment of Civil Engineering, University of OttawaChildren’s Hospital of Eastern Ontario Research InstituteDepartment of Civil Engineering, University of OttawaChildren’s Hospital of Eastern Ontario Research InstituteChildren’s Hospital of Eastern Ontario Research InstituteDepartment of Civil Engineering, University of OttawaDepartment of Civil Engineering, University of OttawaAbstract Recurrent influenza epidemics and pandemic potential are significant risks to global health. Public health authorities use clinical surveillance to locate and monitor influenza and influenza-like cases and outbreaks to mitigate hospitalizations and deaths. Currently, global integration of clinical surveillance is the only reliable method for reporting influenza types and subtypes to warn of emergent pandemic strains. The utility of wastewater surveillance (WWS) during the COVID-19 pandemic as a less resource intensive replacement or complement for clinical surveillance has been predicated on analyzing viral fragments in wastewater. We show here that influenza virus targets are stable in wastewater and partitions favorably to the solids fraction. By quantifying, typing, and subtyping the virus in municipal wastewater and primary sludge during a community outbreak, we forecasted a citywide flu outbreak with a 17-day lead time and provided population-level viral subtyping in near real-time to show the feasibility of influenza virus WWS at the municipal and neighbourhood levels in near real time using minimal resources and infrastructure.https://doi.org/10.1038/s41598-022-20076-z |
| spellingShingle | Elisabeth Mercier Patrick M. D’Aoust Ocean Thakali Nada Hegazy Jian-Jun Jia Zhihao Zhang Walaa Eid Julio Plaza-Diaz Md Pervez Kabir Wanting Fang Aaron Cowan Sean E. Stephenson Lakshmi Pisharody Alex E. MacKenzie Tyson E. Graber Shen Wan Robert Delatolla Municipal and neighbourhood level wastewater surveillance and subtyping of an influenza virus outbreak Scientific Reports |
| title | Municipal and neighbourhood level wastewater surveillance and subtyping of an influenza virus outbreak |
| title_full | Municipal and neighbourhood level wastewater surveillance and subtyping of an influenza virus outbreak |
| title_fullStr | Municipal and neighbourhood level wastewater surveillance and subtyping of an influenza virus outbreak |
| title_full_unstemmed | Municipal and neighbourhood level wastewater surveillance and subtyping of an influenza virus outbreak |
| title_short | Municipal and neighbourhood level wastewater surveillance and subtyping of an influenza virus outbreak |
| title_sort | municipal and neighbourhood level wastewater surveillance and subtyping of an influenza virus outbreak |
| url | https://doi.org/10.1038/s41598-022-20076-z |
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